SERS microscopic imaging as novel tool for assessing viability and enumerating yeast cells at various stages of cell cycle in lag, log, exponential and stationary phases of growth in culture
Source abstract
Abstract
Surface enhanced Raman scattering (SERS) microscopic imaging was employed to enumerate the yeast cells in culture. We found this imaging method as an efficient tool for easily differentiating and quantitatively enumerating yeast cell at different stages of cell-division cycle (G1, S, G2 and M phase) at various stages of growth phases namely lag, log, exponential and stationary phases in culture. Apart from enumerating the cells at different stages of cell cycle under lag, log, exponential and stationary phases, it was possible using SERS microscopy to differentiate the live cells from dead ones. The dead cells were SERS inactive and gave enhanced autofluorescence compared with the live cells, which were SERS active. The results from the present investigation suggest that SERS microscopic imaging, using silver nanoparticles (AgNPs) as a sensitive tool to enumerate the yeast cells in culture.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Spectroscopy Techniques in Biomedical and Chemical Research · Gold and Silver Nanoparticles Synthesis and Applications · Listeria monocytogenes in Food Safety
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
CSIR-CLRI
Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
References (27)
Show source-provided references
1. 10.1016/j.talanta.2008.02.04210.1016/j.talanta.2008.02.042 ↗
2. 10.2174/13892011079224648310.2174/138920110792246483 ↗
3. 10.1002/cphc.20090011910.1002/cphc.200900119 ↗
4. 10.1016/S0378-5173(97)00120-810.1016/S0378-5173(97)00120-8 ↗
5. 10.1021/nl061517x10.1021/nl061517x ↗
6. 10.1021/ac902364h10.1021/ac902364h ↗
7. 10.1007/s00216-009-2682-310.1007/s00216-009-2682-3 ↗
8. 10.1016/j.nano.2009.07.00910.1016/j.nano.2009.07.009 ↗
9. 10.1007/3-540-33567-610.1007/3-540-33567-6 ↗
10. 10.1023/A:101601312309410.1023/A:1016013123094 ↗
11. Ma , D . 2001 . Applications of yeast in drug discovery . Prog. Drug Res , 57 : 117 – 162 .
12. 10.4161/bbug.1968710.4161/bbug.19687 ↗
13. 10.1094/ASBCJ-57-000110.1094/ASBCJ-57-0001 ↗
14. 10.1099/00221287-139-4-83510.1099/00221287-139-4-835 ↗
15. 10.1128/JCM.39.2.478-484.200110.1128/JCM.39.2.478-484.2001 ↗
16. Smart , K A , Chambers , K M , Lambert , I , Jenkins , C and Smart , C A . 1999 . Use of methylene violet staining procedures to determine yeast viability and vitality . J. Am , 57 : 18 – 23 .
17. 10.1146/annurev.food.080708.10073010.1146/annurev.food.080708.100730 ↗
18. O’ Connor-Cox , E , Mochaba , F M , Lodolo , E J , Majara , M and Axcell , B . 1997 . Methylene blue staining: use at your own risk . Tech. Q Master , 34 : 306 – 312 .
19. 10.1021/la300424510.1021/la3004245 ↗
20. 10.1007/s00216-009-2883-910.1007/s00216-009-2883-9 ↗
21. Tkacz J S Cybulska E B and Lampen J O . 1971 . Specific staining of wall mannan in yeast cells with fluorescein-conjugated concanavalin A . 105 : 1 – 5 .10.1128/jb.105.1.1-5.1971 ↗
22. 10.1016/0922-338X(94)90292-510.1016/0922-338X(94)90292-5 ↗
23. Wei , W , Nurse , P and Broek , D . 1993 . Yeast cells can enter a quiescent state through G1, S, G2 or M phase of the cell cycle . Cancer Res , 53 : 1867 – 1870 .
24. 10.1016/S1369-5274(02)00369-710.1016/S1369-5274(02)00369-7 ↗
25. 10.1016/j.cell.2011.11.00210.1016/j.cell.2011.11.002 ↗
26. 10.1016/j.saa.2006.07.03510.1016/j.saa.2006.07.035 ↗
27. 10.1016/j.mimet.2009.01.01110.1016/j.mimet.2009.01.011 ↗