Dual-crosslinked in-situ forming alginate/silk fibroin hydrogel with potential for bone tissue engineering
Source abstract
Abstract
This study aimed to improve the mechanical and biological properties of alginate-based hydrogels. For this purpose, in-situ forming hydrogels were prepared by dual crosslinking of Alginate (Alg)/Oxidized Alginate (OAlg)/Silk Fibroin (SF) through simultaneous ionic gelation using CaCO3-GDL and Schiff-base reaction. The resulting hydrogels were characterized by FTIR, SEM, compressive modulus, and rheological tests. Compared to the physically-crosslinked alginate hydrogel, the compressive modulus of dual-crosslinked Alg/OAlg/SF hydrogel increased from 28 to 67 kPa, due to the covalent imine bond formation. Then, MTT and DAPI staining assays were performed to demonstrate the biocompatibility of hydrogel. Furthermore, the differentiation potential of bone marrow mesenchymal stem cells encapsulated in hydrogel scaffolds to bone tissue was tested by ALP activity, Alizarin Red staining, and real-time PCR. The overall results showed the potential of Alginate/Oxidized Alginate/Silk Fibroin hydrogel scaffold for bone tissue engineering applications.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Silk-based biomaterials and applications · Bone Tissue Engineering Materials · Hydrogels: synthesis, properties, applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (46)
Show source-provided references
1. Biomaterials for bone tissue engineering scaffolds: a review10.1039/C9RA05214C ↗
2. Tissue engineering strategies to increase osteochondral regeneration of stem cells; a close look at different modalities10.1007/s12015-021-10130-0 ↗
3. Hydrogel as a biomaterial for bone tissue engineering: a review10.3390/nano10081511 ↗
4. In-situ forming, cytocompatible, and self-recoverable tough hydrogels based on dual ionic and click crosslinked alginate10.1021/acs.biomac.8b00140 ↗
5. Alginate membrane dressing toughened by chitosan floccule to load antibacterial drugs for wound healing10.1016/j.polymertesting.2019.106039 ↗
6. Hydrogels as intelligent materials: a brief review of synthesis, properties and applications10.1016/j.mtchem.2018.02.004 ↗
7. A review on the construction of hydrogel scaffolds by various chemically techniques for tissue engineering10.1016/j.eurpolymj.2019.05.004 ↗
8. Hydrogels based on schiff base linkages for biomedical applications10.3390/molecules24163005 ↗
9. Towards non-stick silk: tuning the hydrophobicity of silk fibroin protein
10. Silk fibroin as biomaterial for bone tissue engineering10.1016/j.actbio.2015.09.005 ↗
11. Application of injectable silk fibroin/graphene oxide hydrogel combined with bone marrow mesenchymal stem cells in bone tissue engineering10.1016/j.colsurfa.2020.125318 ↗
12. Mineralized alginate hydrogels using marine carbonates for bone tissue engineering applications10.1016/j.carbpol.2018.04.101 ↗
13. Fabrication of oxidized alginate-gelatin-BCP hydrogels and evaluation of the microstructure, material properties and biocompatibility for bone tissue regeneration10.1177/0885328211404265 ↗
14. Injectable polysaccharide hydrogel embedded with hydroxyapatite and calcium carbonate for drug delivery and bone tissue engineering10.1016/j.ijbiomac.2018.06.200 ↗
15. Alginate hydrogels for bone tissue engineering, from injectables to bioprinting: a review10.1016/j.carbpol.2019.115514 ↗
16. Applications of oxidized alginate in regenerative medicine10.1039/D0TB02691C ↗
17. Oxidized alginate-based hydrogels for tissue engineering applications: a review10.1021/acs.biomac.7b01331 ↗
18. Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: a novel scaffold with biological and antibacterial activity and improved mechanical properties10.1016/j.ijbiomac.2020.08.090 ↗
19. Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties10.1016/j.carbpol.2020.116802 ↗
20. Silk fibroin-alginate-hydroxyapatite composite particles in bone tissue engineering applications in vivo10.3390/ijms18040858 ↗
21. Alginate-poloxamer/silk fibroin hydrogels with covalently and physically crosslinked networks for cartilage tissue engineering10.1016/j.carbpol.2020.116593 ↗
22. Biocompatible silk/calcium silicate/sodium alginate composite scaffolds for bone tissue engineering10.1016/j.carbpol.2018.06.093 ↗
23. Cytoprotection of human progenitor and stem cells through encapsulation in alginate templated, dual crosslinked silk and silk–gelatin composite hydrogel microbeads10.1002/adhm.202200293 ↗
24. Oxidized sodium alginate crosslinked silk fibroin composite scaffold for skin tissue engineering10.1002/jbm.b.35119 ↗
25. The effect of sterilization on silk fibroin biomaterial properties10.1002/mabi.201500013 ↗
26. Fabrication of functionalized citrus pectin/silk fibroin scaffolds for skin tissue engineering10.1002/jbm.b.34079 ↗
27. Self-crosslinking effect of chitosan and gelatin on alginate based hydrogels: injectable in situ forming scaffolds10.1016/j.msec.2018.04.018 ↗
28. Controlling alginate oxidation conditions for making alginate-gelatin hydrogels10.1016/j.carbpol.2018.06.080 ↗
29. Characterization of the microstructure of hydrazone crosslinked polysaccharide-based hydrogels through rheological and diffusion studies
30. pH-responsive, dynamically restructuring hydrogels formed by reversible crosslinking of PVA with phenylboronic acid functionalised PPO–PEO–PPO spacers10.1039/c1sm06230a ↗
31. Modified hydroxyapatite nanoparticles reinforced nanocomposite hydrogels based on gelatin/oxidized alginate via Schiff base reaction
32. A soft tissue adhesive based on aldehyde-sodium alginate and amino-carboxymethyl chitosan preparation through the Schiff reaction10.1007/s11706-017-0392-x ↗
33. High-strength functionalized pectin/fibroin hydrogel with tunable properties: a structure–property relationship study10.1002/app.48859 ↗
34. A biomimetic silk fibroin/sodium alginate composite scaffold for soft tissue engineering10.1038/srep39477 ↗
35. Controlled gelation and degradation rates of injectable hyaluronic acid-based hydrogels through a double crosslinking strategy10.1002/term.378 ↗
36. A hydrogel/fiber scaffold based on silk fibroin/oxidized pectin with sustainable release of vancomycin hydrochloride10.1016/j.eurpolymj.2019.06.001 ↗
37. Salt-assisted toughening of protein hydrogel with controlled degradation for bone regeneration10.1002/adfm.201901314 ↗
38. Scaffolds for bone healing: concepts, materials and evidence10.1016/j.injury.2011.03.033 ↗
39. Bioactive hydrogels for bone regeneration10.1016/j.bioactmat.2018.05.006 ↗
40. Preparation of covalently bonded silica-alginate hybrid hydrogels by SCHIFF base and sol-gel reactions10.1016/j.carbpol.2021.118186 ↗
41. Gellan gum/alginate-based Ca-enriched acellular bilayer hydrogel with robust interface bonding for effective osteochondral repair10.1016/j.carbpol.2021.118382 ↗
42. Injectable chitosan hydrogel embedding modified halloysite nanotubes for bone tissue engineering10.1016/j.carbpol.2021.118311 ↗
43. An injectable, electroconductive hydrogel/scaffold for neural repair and motion sensing10.1021/acs.chemmater.0c02906 ↗
44. Alendronate has an anabolic effect on bone through the differentiation of mesenchymal stem cells10.1359/jbmr.070701 ↗
45. Microfluidic fabrication of alendronate-loaded chitosan nanoparticles for enhanced osteogenic differentiation of stem cells10.1016/j.lfs.2020.117768 ↗
46. Co-electrospun silk fibroin and gelatin methacryloyl sheet seeded with mesenchymal stem cells for tendon regeneration10.1002/smll.202107714 ↗