Analysis of osteoblast mineral deposits on orthopaedic/dental implant metals
Source abstract
Abstract
Neonatal rat calvarial osteoblasts were cultured on Ti-6Al-4V, Co-Cr-Mo alloy, 316L stainless steel and polystyrene (reference substrate) in the presence of ascorbic acid and 10 mM beta-glycerophosphate for 16, 17, 18, 19, 20, 21, 24 and 28 d. Scanning electron microscopy examination revealed that osteoblasts cultured on these orthopaedic/dental implant metals synthesized and deposited an extracellular matrix containing collagenous and non-collagenous components, as well as mineral nodules of various morphologies. Energy dispersive spectrometry revealed that the mineral deposits consisted of three distinct chemical compositions: calcium phosphate, calcium-sulphur-phosphorus, and calcium only. Backscattered electron imaging demonstrated that both the calcium phosphate and calcium-only deposits were electron dense, while the calcium-sulphur-phosphorus deposits were electron translucent. X-ray diffraction analysis indicated that the bulk of the osteoblast mineral deposits was amorphous hydroxyapatite; in addition, electron diffraction analysis revealed small regions of crystalline hydroxyapatite.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Orthopaedic implants and arthroplasty · Titanium Alloys Microstructure and Properties
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (24)
Show source-provided references
1. Osteoblast responses to orthopedic implant materials in vitro10.1002/jbm.820250603 ↗
2. Osteoblasts on hydroxyapatite, alumina and bone surfaces in vitro: morphology during the first 2 h of attachment10.1016/0142-9612(92)90008-C ↗
3. Examination of osteoblast-orthopaedic biomaterial interactions using molecular techniques10.1016/0142-9612(93)90220-V ↗
4. Transmission electron microscopy examination of the interface between osteoblasts and various orthopedic biomaterials
5. A method for transmission electron microscopy investigation of the osteoblast/ hydroxyapatite interface10.1002/jab.770050106 ↗
6. A transmission electron microscopy examination of the interface between osteoblasts and metal biomaterials10.1002/jbm.820290810 ↗
7. Analysis of osteoblast mineral deposits on orthopaedic/dental implant metals
8. Physiological and pharmacological regulation of biological calcification10.1016/S0074-7696(08)60685-3 ↗
9. Noncollagenous proteins and their role in substrate induced bioactivity
10. Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix10.1002/jcp.1041430304 ↗
11. Relationship of cell growth to the regulation of tissue-specific gene expression during osteoblast differentiation10.1096/fasebj.4.13.2210157 ↗
12. Collagen expression, ultrastructural assembly, and mineralization in cultures of chicken embryo osteoblasts10.1083/jcb.106.3.979 ↗
13. Mineralization in osteoblast cultures: a light and electron microscopic study10.1016/8756-3282(88)90004-X ↗
14. Formation of bone tissue in culture from isolated bone cells10.1083/jcb.61.2.427 ↗
15. Three-dimensional spatial relationship between the collagen fibrils and inorganic calcium-phosphate crystals of pickerel and herring bone10.1016/0022-2836(91)90752-R ↗
16. The role of implant surface geometry on mineralization in vivo and in vitro; a transmission and scanning electron microscopic study
17. Mineralized matrix production by osteoblasts on solid titanium in vitro
18. Bone-forming cells in clinical conditions
19. Calcium-phosphate biomaterials and bone mineral. Differences in composition, structure, and properties
20. Bone mineral and the mineralization process
21. Mechanical stimulation by intermittent compression stimulates sulfate incorporation and matrix mineralization in fetal mouse long-bone rudiments under serum-free conditions
22. An electron microscopical study on the presence of proteoglycans in the calcified bone matrix by use of cuprolinic blue10.1016/8756-3282(89)90066-5 ↗
23. Acidic phosphoproteins from bone matrix: a structural rationalization of their role in biomineralization10.1007/BF00296767 ↗
24. Mineral-matrix interaction in bone and cartilage10.1097/00003086-199208000-00041 ↗