Robust and customizable spheroid culture system for regenerative medicine
Source abstract
Abstract
Abstract Three-dimensional cell spheroids show promise for the reconstruction of native tissues. Herein, we report a sophisticated, uniform, and highly reproducible spheroid culture system for tissue reconstruction. A mesh-integrated culture system was designed to precisely control the uniformity and reproducibility of spheroid formation. Furthermore, we synthesized hexanoyl glycol chitosan, a material with ultralow cell adhesion properties, to further improve spheroid formation efficiency and biological function. Our results demonstrate improved biological function in various types of cells and ability to generate spheroids with complex structures composed of multiple cell types. In conclusion, our spheroid culture system offers a highly effective and widely applicable approach to generating customized spheroids with desired structural and biological features for a variety of biomedical applications.
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Research context
OpenAlex topics: 3D Printing in Biomedical Research · Pluripotent Stem Cells Research · Additive Manufacturing and 3D Printing Technologies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (41)
Show source-provided references
1. Stem cells in gastroenterology and hepatology10.1038/nrgastro.2009.195 ↗
2. Fabrication and characterization techniques of in vitro 3D tissue models10.3390/ijms24031912 ↗
3. Three-dimensional spheroids as in vitro preclinical models for cancer research10.3390/pharmaceutics12121186 ↗
4. Engineering organoids10.1038/s41578-021-00279-y ↗
5. Cell aggregate assembly through microengineering for functional tissue emergence10.3390/cells11091394 ↗
6. Spheroid culture system methods and applications for mesenchymal stem cells10.3390/cells8121620 ↗
7. 3D tumor spheroid models for in vitro therapeutic screening: a systematic approach to enhance the biological relevance of data obtained10.1038/srep19103 ↗
8. Hydrogels with an embossed surface: an all-in-one platform for mass production and culture of human adipose-derived stem cell spheroids10.1016/j.biomaterials.2018.10.025 ↗
9. Taking the study of cancer cell survival to a new dimension10.1016/S0092-8674(02)01229-1 ↗
10. The microwell-mesh: a high-throughput 3D prostate cancer spheroid and drug-testing platform10.1038/s41598-017-18050-1 ↗
11. Bioinspired tuning of glycol chitosan for 3D cell culture10.1038/am.2016.130 ↗
12. Non-cell adhesive hexanoyl glycol chitosan hydrogels for stable and efficient formation of 3D cell spheroids with tunable size and density10.1016/j.ijbiomac.2021.07.185 ↗
13. Application of hexanoyl glycol chitosan as a non-cell adhesive polymer in three-dimensional cell culture10.1021/acsomega.2c00890 ↗
14. Synthesis and characterization of a new photo-crosslinkable glycol chitosan thermogel for biomedical applications10.1016/j.carbpol.2016.02.029 ↗
15. Thermo-sensitive injectable glycol chitosan-based hydrogel for treatment of degenerative disc disease10.1016/j.carbpol.2018.01.006 ↗
16. The effect of hexanoyl glycol chitosan on the proliferation of human mesenchymal stem cells10.3390/polym10080839 ↗
17. Facile tumor spheroids formation in large quantity with controllable size and high uniformity10.1038/s41598-018-25203-3 ↗
18. Engineered biomaterials to guide spheroid formation, function, and fabrication into 3D tissue constructs10.1016/j.actbio.2022.09.052 ↗
19. Optimizing human embryonic stem cells differentiation efficiency by screening size-tunable homogenous embryoid bodies10.1016/j.biomaterials.2014.04.001 ↗
20. Generation of multicellular tumor spheroids with microwell-based agarose scaffolds for drug testing10.1371/journal.pone.0130348 ↗
21. Uniform tumor spheroids on surface-optimized microfluidic biochips for reproducible drug screening and personalized medicine10.3390/mi13040587 ↗
22. Investigating PEGDA and GelMA microgel models for sustained 3D heterotypic dermal papilla and keratinocyte co-cultures10.3390/ijms22042143 ↗
23. Microfluidic device for enhancement and analysis of osteoblast differentiation in three-dimensional cell cultures10.1186/s13036-023-00395-z ↗
24. 3D cultures of prostate cancer cells cultured in a novel high-throughput culture platform are more resistant to chemotherapeutics compared to cells cultured in monolayer10.1371/journal.pone.0111029 ↗
25. Applications of biomaterials in 3D cell culture and contributions of 3D cell culture to drug development and basic biomedical research10.3390/ijms22052491 ↗
26. Challenges of applying multicellular tumor spheroids in preclinical phase10.1186/s12935-021-01853-8 ↗
27. Critical role of three-dimensional tumorsphere size on experimental outcome10.2144/btn-2020-0081 ↗
28. Advances in the formation, use and understanding of multi-cellular spheroids10.1517/14712598.2012.707181 ↗
29. Engineering multi-cellular spheroids for tissue engineering and regenerative medicine10.1002/adhm.202000608 ↗
30. Modelling the complex nature of the tumor microenvironment: 3D tumor spheroids as an evolving tool10.1186/s12929-024-00997-9 ↗
31. Cardiac spheroids as in vitro bioengineered heart tissues to study human heart pathophysiology10.3791/61962 ↗
32. Scaffold-free cell-based tissue engineering therapies: advances, shortfalls and forecast10.1038/s41536-021-00133-3 ↗
33. Dynamic conversion of cell sorting patterns in aggregates of embryonic stem cells with differential adhesive affinity10.1186/s12861-020-00234-0 ↗
34. An overview of substrate stiffness guided cellular response and its applications in tissue regeneration10.1016/j.bioactmat.2021.12.005 ↗
35. Expression of adhesion and extracellular matrix genes in human blastocysts upon attachment in a 2D co-culture system10.1093/molehr/gay024 ↗
36. Cooperative coupling of cell-matrix and cell–cell adhesions in cardiac muscle10.1073/pnas.1203007109 ↗
37. Low-adhesion culture selection for human iPS cell-derived cardiomyocytes10.1038/s41598-024-60765-5 ↗
38. Structural and functional analysis of cell adhesion and nuclear envelope nano-topography in cell death10.1038/srep15623 ↗
39. Potential effects of nonadherent on adherent human umbilical venous endothelial cells in cell culture10.3390/ijms22031493 ↗
40. Loss of matrix adhesion triggers rapid transformation-selective apoptosis in fibroblasts10.1083/jcb.138.4.901 ↗
41. Cardiomyocytes in vitro adhesion is actively influenced by biomimetic synthetic peptides for cardiac tissue engineering10.1089/ten.tea.2011.0254 ↗