Biocompatibility of surfactant‐templated polyurea–nanoencapsulated macroporous silica aerogels with plasma platelets and endothelial cells

Abstract The recently synthesized polyurea‐nanoencapsulated surfactant‐templated aerogels (X‐aerogels) are porous materials with significantly improved mechanical strengths. Surface‐wise they resemble polyurethane, a common biocompatible material, but their biocompatibility has never been investigated. As lightweight and strong materials, if X‐aerogels also have acceptable biocompatibility, they may be used in many implantable devices. The goal of this study was to investigate their biocompatibility toward platelets, blood plasma, and vascular endothelial cells, in terms of cell activation and inflammatory responses. Platelets were incubated with X‐aerogel and platelet activation was measured through CD62P and phosphatidylserine expression. Platelet aggregation was also measured. Contact with X‐aerogel did not induce platelet activation or impair aggregation. To determine X‐aerogel‐induced inflammation, plasma anaphylatoxin C3a level was measured after incubation with X‐aerogel. Results showed that X‐aerogel induced no changes in plasma C3a levels. SEM and SDS–PAGE were used to examine cellular/protein deposition on X‐aerogel samples after plasma incubation. No structural change or organic deposition was detected. Furthermore, X‐aerogel samples did not induce any significant changes in vascular endothelial cell culture parameters after 5 days of incubation. These observations suggest that X‐aerogels have a suitable biocompatibility toward platelets, plasma, and vascular endothelial cells, and they have potential for use in blood implantable devices. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010