DataVXJournal of Biomedical Materials Research Part APaper
journal article·2017-05

Evaluation of PVA biodegradable electric conductive membranes for nerve regeneration in axonotmesis injuries: the rat sciatic nerve animal model

Jorge Ribeiro, Ana Rita Caseiro, Tiago Pereira, Paulo Alexandre Armada‐da‐Silva, Isabel Pires, Justina Prada, Irina Amorim, Inês Leal Reis, Sandra Amado, José Domingos Santos, Simone Bompasso, Stefania Raimondo, Artur Severo Proença Varejão, Stefano Geuna, Ana Lúcia Luís, Ana Colette Maurício

Journal of Biomedical Materials Research Part A· Volume 105· Issue 5
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Source abstract

Abstract

Abstract The therapeutic effect of three polyvinyl alcohol (PVA) membranes loaded with electrically conductive materials ‐ carbon nanotubes (PVA‐CNTs) and polypyrrole (PVA‐PPy) ‐ were tested in vivo for neuro‐muscular regeneration after an axonotmesis injury in the rat sciatic nerve. The membranes electrical conductivity measured was 1.5 ± 0.5 × 10−6 S/m, 579 ± 0.6 × 10−6 S/m, and 1837.5 ± 0.7 × 10−6 S/m, respectively. At week‐12, a residual motor and nociceptive deficit were present in all treated groups, but at week‐12, a better recovery to normal gait pattern of the PVA‐CNTs and PVA‐PPy treated groups was observed. Morphometrical analysis demonstrated that PVA‐CNTs group presented higher myelin thickness and lower g‐ratio. The tibialis anterior muscle, in the PVA‐PPy and PVA‐CNTs groups showed a 9% and 19% increase of average fiber size area and a 5% and 10% increase of the “minimal Feret's diameter,” respectively. No inflammation, degeneration, fibrosis or necrosis were detected in lung, liver, kidneys, spleen, and regional lymph nodes and absence of carbon deposits was confirmed with Von Kossa and Masson‐Fontana stains. In conclusion, the membranes of PVA‐CNTs and PVA‐PPy are biocompatible and have electrical conductivity. The higher electrical conductivity measured in PVA‐CNTs membrane might be responsible for the positive results on maturation of myelinated fibers. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1267–1280, 2017.
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Research context

NanomaterialsBiomaterials

OpenAlex topics: Nerve injury and regeneration · Electrospun Nanofibers in Biomedical Applications · Cerebral Palsy and Movement Disorders

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Departmento de Clínicas Veterinárias Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP) Rua de Jorge Viterbo Ferreira, nº 228 Porto 4050‐313 Portugal

Sub‐inidade de Cirurgia Experimental e Medicina Regenerativa Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências, Tecnologias e Agroambiente da Universidade do Porto (ICETA) Rua D. Manuel II, Apartado 55142 Porto 4051‐401 Portugal

UPVET, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS) Universidade do Porto (UP) Rua de Jorge Viterbo Ferreira, nº 228 Porto 4050‐313 Portugal

CEMUC, Departamento de Engenharia Metalúrgica e Materiais Faculdade de Engenharia, Universidade do Porto Rua Dr. Roberto Frias Porto 4200‐465 Portugal

Faculdade de Motricidade Humana (FMH), Universidade de Lisboa (ULisboa) Estrada da Costa, 1499‐002 Dafundo Cruz Quebrada Portugal

CIPER‐FMH: Centro Interdisciplinar de Estudo de Performance Humana, Faculdade de Motricidade Humana (FMH), Universidade de Lisboa (ULisboa) Estrada da Costa 1499‐002 Cruz Quebrada – Dafundo Portugal

Departamento de Ciências Veterinárias, Universidade de Trás‐os‐Montes e Alto Douro (UTAD), Quinta de Prados 5000‐801 Vila Real Portugal

CECAV, Centro de Ciência Animal e Veterinária Universidade de Trás‐os‐Montes e Alto Douro (UTAD) Quinta de Prados Vila Real 5000‐801 Portugal

Departmento de Patologia e de Imunologia Molecular Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP) Rua de Jorge Viterbo Ferreira, nº 228 Porto 4050‐313 Portugal

Instituto de Patologia e Imunologia Molecular da Universidade do Porto (IPATIMUP) Rua Dr. Roberto Frias s/n 4200‐465, Porto Portugal

Instituto de Investigação e Inovação em Saúde (i3S), Universidade do Porto (UP) Rua Alfredo Allen Porto 4200‐135 Portugal

Instituto Politécnico de Leiria UIS‐IPL: Unidade de Investigação em Saúde da Escola Superior de Saúde de Leiria Portugal

CDrsp ‐ Centre for Rapid and Sustainable Product Development Rua de Portugal 2430‐028 Marinha Grande Portugal

Department of Clinical and Biological Sciences University of Turin Turin 10126 Italy

Neuroscience Institute of the Cavalieri Ottolenghi Foundation (NICO), Azienda Ospedaliero‐Universitaria San Luigi Gonzaga Regione Gonzole 10 Orbassano 10043 Turin Italy

Funding

Programa Operacional Regional do Norte · QREN · “iBone Therapies: Terapias inovadoras para a regeneração óssea” · FCT · FEDER

References (51)

Show source-provided references
1. 10.5772/5333510.5772/53335 ↗
2. 10.1155/2014/30265910.1155/2014/302659 ↗
3. Spinal nerve root repair after brachial plexus injury
4. Use of hybrid chitosan membranes and human mesenchymal stem cells from the Wharton jelly of umbilical cord for promoting nerve regeneration in an axonotmesis rat model
5. 10.5772/5333610.5772/53336 ↗
6. 10.1155/2014/37691810.1155/2014/376918 ↗
7. Effects of umbilical cord tissue mesenchymal stem cells (UCX(R)) on rat sciatic nerve regeneration after neurotmesis injuries10.46582/jsrm.1001004 ↗
8. 10.1016/j.diff.2012.10.00110.1016/j.diff.2012.10.001 ↗
9. 10.1016/B978-0-12-410499-0.00004-610.1016/B978-0-12-410499-0.00004-6 ↗
10. 10.1002/micr.2031710.1002/micr.20317 ↗
11. 10.1089/ten.tea.2007.027310.1089/ten.tea.2007.0273 ↗
12. Evaluation of two biodegradable nerve guides for the reconstruction of the rat sciatic nerve
13. 10.4252/wjsc.v7.i6.95610.4252/wjsc.v7.i6.956 ↗
14. 10.1002/jbm.a.3222610.1002/jbm.a.32226 ↗
15. 10.1002/jbm.b.3269410.1002/jbm.b.32694 ↗
16. Dielectric properties of poly (vinyl alcohol)/polypyrrole composite polymer films
17. 10.1002/term.38310.1002/term.383 ↗
18. MartiniF BartholomewE.Essentials of Anatomy and Physiology 5th ed. New York: Benjamin Cummings;2009.
19. 10.1006/exnr.1994.109310.1006/exnr.1994.1093 ↗
20. 10.1371/journal.pone.011376910.1371/journal.pone.0113769 ↗
21. 10.1186/1743-0003-7-710.1186/1743-0003-7-7 ↗
22. 10.1016/j.biomaterials.2008.07.04310.1016/j.biomaterials.2008.07.043 ↗
23. 10.7150/ijms.913910.7150/ijms.9139 ↗
24. 10.1002/jbm.a.3573010.1002/jbm.a.35730 ↗
25. 10.1089/neu.2004.21.165210.1089/neu.2004.21.1652 ↗
26. 10.1016/j.jneumeth.2007.02.01710.1016/j.jneumeth.2007.02.017 ↗
27. 10.1055/s-2001-1775510.1055/s-2001-17755 ↗
28. 10.1097/00000542-199308000-0002010.1097/00000542-199308000-00020 ↗
29. LuísAL.Reparação de lesões do nervo periférico num modelo animal in Veterinary Clinics. Universidade do Porto;2008. Portugal p269.
30. Maurício AC · 2011 · 978
31. 10.1016/S0304-3959(00)00388-210.1016/S0304-3959(00)00388-2 ↗
32. 10.1179/01616410422501383310.1179/016164104225013833 ↗
33. 10.1097/00000542-199504000-0002610.1097/00000542-199504000-00026 ↗
34. 10.1006/exnr.2000.760010.1006/exnr.2000.7600 ↗
35. 10.1097/00006534-198901000-0002410.1097/00006534-198901000-00024 ↗
36. 10.1016/S0165-0270(00)00281-810.1016/S0165-0270(00)00281-8 ↗
37. 10.1002/mus.1037410.1002/mus.10374 ↗
38. 10.1016/S0014-4886(03)00208-510.1016/S0014-4886(03)00208-5 ↗
39. 10.1002/micr.1019910.1002/micr.10199 ↗
40. 10.1016/S0940-9602(00)80117-X10.1016/S0940-9602(00)80117-X ↗
41. 10.2217/17435889.3.2.14910.2217/17435889.3.2.149 ↗
42. 10.1016/S0379-6779(03)00360-610.1016/S0379-6779(03)00360-6 ↗
43. 10.1097/00000637-198909000-0000210.1097/00000637-198909000-00002 ↗
44. 10.1002/micr.1014710.1002/micr.10147 ↗
45. 10.1002/jbm.a.3509810.1002/jbm.a.35098 ↗
46. 10.1002/hep.2575310.1002/hep.25753 ↗
47. 10.1007/BF0033567810.1007/BF00335678 ↗
48. 10.1016/S0074-7742(09)87007-410.1016/S0074-7742(09)87007-4 ↗
49. 10.1016/0014-4886(65)90022-110.1016/0014-4886(65)90022-1 ↗
50. 10.1089/biores.2013.001210.1089/biores.2013.0012 ↗
51. 10.1016/j.neulet.2014.04.02910.1016/j.neulet.2014.04.029 ↗