DataVXJournal of Biomaterials Science, Polymer EditionPaper
journal article·2011-01

An Injectable Oxidated Hyaluronic Acid/Adipic Acid Dihydrazide Hydrogel as a Vitreous Substitute

Wen-Yu Su, Ko-Hua Chen, Yu-Chun Chen, Yen-Hsien Lee, Ching-Li Tseng, Feng-Huei Lin

Journal of Biomaterials Science, Polymer Edition· Volume 22· Issue 13
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Source abstract

Abstract

Vitrectomy is a common procedure for treating ocular-related diseases. The surgery involves removing the vitreous humor from the center of the eye, and vitreous substitutes are needed to replace the vitreous humor after vitrectomy. In the present study, we developed a colorless, transparent and injectable hydrogel with appropriate refractive index as a vitreous substitute. The hydrogel is formed by oxidated hyaluronic acid (oxi-HA) cross-linked with adipic acid dihydrazide (ADH). Hyaluronic acid (HA) was oxidized by sodium periodate to create aldehyde functional groups, which could be cross-linked by ADH. The refractive index of this hydrogel ranged between 1.3420 and 1.3442, which is quite similar to human vitreous humor (1.3345). The degradation tests demonstrated that the hydrogel could maintain the gel matrix over 35 days, depending on the ADH concentration. In addition, the cytotoxicity was evaluated on retina pigmented epithelium (RPE) cells cultivated following the ISO standard (tests for in vitro cytotoxicity), and the hydrogel was found to be non-toxic. In a preliminary animal study, the oxi-HA/ADH hydrogel was injected into the vitreous cavity of rabbit eyes. The evaluations of slit-lamp observation, intraocular pressure, cornea thickness and histological examination showed no significant abnormal biological reactions for 3 weeks. This study suggests that the injectable oxi-HA/ADH hydrogel should be a potential vitreous substitute.
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Research context

Polymers

OpenAlex topics: Retinal and Macular Surgery · Glaucoma and retinal disorders · Intraocular Surgery and Lenses

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

a Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, R.O.C.; Division of Medical Engineering Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan, R.O.C.

b Division of Medical Engineering Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan, R.O.C.; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan, R.O.C.; National Yang-Ming University, Taipei, Taiwan, R.O.C.

c Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, R.O.C.; Division of Medical Engineering Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan, R.O.C.

d Division of Medical Engineering Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan, R.O.C.

e Division of Medical Engineering Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan, R.O.C.; National Science Council, Taipei, Taiwan, R.O.C.

f Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, R.O.C.; Division of Medical Engineering Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan, R.O.C. double@nhri.org.tw

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