DataVXJournal of Biomaterials Science, Polymer EditionPaper
journal article·2018-03-24

Nuclear targeting peptide-modified, DOX-loaded, PHBV nanoparticles enhance drug efficacy by targeting to Saos-2 cell nuclear membranes

Ayla Şahin, Gozde Eke, Arda Buyuksungur, Nesrin Hasirci, Vasif Hasirci

Journal of Biomaterials Science, Polymer Edition· Volume 29· Issue 5
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Source abstract

Abstract

The aim of this study was to target nano sized (266 ± 25 nm diameter) poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) particles carrying Doxorubicin (DOX), an anticancer agent, to human osteosarcoma cells (Saos-2). A nuclear targeting molecule (Nuclear Localization Signal, NLS), a 17 a.a. peptide, was attached onto the doxorubicin loaded nanoparticles. NLS conjugated nanoparticles surrounded the cell nuclei, but did not penetrate them. Free doxorubicin and doxorubicin loaded nanoparticles entered the cytoplasm and were evenly distributed within the cytoplasm. The localization of the NLS-targeted particles around the nuclear membrane caused a significantly higher decrease in the cancer cell numbers due to apoptosis or necrosis than the untargeted and free doxorubicin formulations showing the importance of targeting the nanoparticles to the nuclear membrane in the treatment of cancer.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: RNA Interference and Gene Delivery · Nanoparticle-Based Drug Delivery · Graphene and Nanomaterials Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biotechnology, Middle East Technical University (METU), Ankara, Turkey

BIOMATEN, METU Center of Excellence in Biomaterials and Tissue Engineering, Ankara, Turkey

Faculty of Arts and Sciences, Department of Chemistry, Ahi Evran University, Kirsehir, Turkey

Department of Chemistry, Middle East Technical University (METU), Ankara, Turkey

Department of Biological Sciences, Middle East Technical University (METU), Ankara, Turkey

Funding

BIOMATEN, METU CoE in Biomaterials and Tissue Engineering · Middle East Technical University

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