journal article·2010-01

Effects of the Surface Characteristics of Polyhydroxyalkanoates on the Metabolic Activities and Morphology of Human Mesenchymal Stem Cells

Bo-Yi Yu, Po-Ya Chen, Yi-Ming Sun, Yu-Tsang Lee, Tai-Horng Young

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Abstract

Polyhydroxyalkanoates (PHAs) are a newer family of biomaterials for tissue-engineering applications. The objective of this study is to investigate the behavior of human mesenchymal stem cells (hMSCs) grown on various PHA films. The surface characteristics of PHA co-polymer films were varied by the content of 3-hydroxyvalerate (HV) or 3-hydroxyhexanoate (HHx) and by the film preparation methods such as compression-molding and solvent-casting. Hyaluronic acid (HA) was further applied to modify the surface properties of PHA membranes. As HV content increased, the crystallinity and the hydrophobicity of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) membranes decreased and the metabolic activity of hMSCs raised, although the distribution and morphology of hMSCs did not show significant variation. Hyaluronic acid (HA) coating on PHA membranes could improve the metabolic activity and reduce the death rate of hMSCs. Aggregates and spheroidal clusters of hMSCs were found on the surface of cast poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) membranes. The growth of hMSCs was remarkably influenced by various surface characteristics of the PHA films.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: biodegradable polymer synthesis and properties · Electrospun Nanofibers in Biomedical Applications · Graphene and Nanomaterials Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

a Institute of Medical Engineering, National Taiwan University, Taipei, Taiwan 100, Republic of China; Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taiwan 320, Republic of China

b Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taiwan 320, Republic of China

c Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taiwan 320, Republic of China; Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Chung-Li, Taiwan 320, Republic of China; R&D Center for Membrane Technology, Chung Yuan University, Chung-Li, Taiwan 320, Republic of China

d Institute of Medical Engineering, National Taiwan University, Taipei, Taiwan 100, Republic of China; Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taiwan 320, Republic of China; Department of Orthopedic Surgery, Min-Sheng General Hospital, Taoyuan, Taiwan 330, Republic of China

e Institute of Medical Engineering, National Taiwan University, Taipei, Taiwan 100, Republic of China

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