journal article·2012-01-01Open access

Time Controlled Release of Arabinofuranosylcytosine (Ara-C) from Agarose Hydrogels using Layer-by-Layer Assembly: An In Vitro Study

Sumit Mehrotra, Daniel Lynam, Chun Liu, Dena Shahriari, Ilsoon Lee, Mark Tuszynski, Jeffrey Sakamoto, Christina Chan

Abstract

Experimentally induced axonal regeneration is compromised by glial scar formation arising from leptomeningeal fibroblasts cells in and around the hydrogel scaffold implanted for nerve repair. Strategies are needed to prevent such fibroblastic reactive cell layer formation for enhanced axonal regeneration. Here, we implement the technique of layer-by-layer assembled degradable, hydrogen bonded multilayers on agarose hydrogels to incorporate an anti-mitotic drug (1-β-D-arabinofuranosylcytosine (Ara-C)) within the agarose hydrogels. We show controlled release of Ara-C under physiological conditions over a period of days. The concentrations of Ara-C released from agarose at the different time points were sufficient to inhibit fibroblast growth in vitro, while not adversely affecting the viability of the neuronal cells.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Nerve injury and regeneration · Graphene and Nanomaterials Applications · Hydrogels: synthesis, properties, applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

a Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA

b Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA

c Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA

d Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA

e Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA

f Center for Neural Repair, Department of Neurosciences, University of California, San Diego, CA 92093, USA

g Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA

h Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA

References (69)

Show source-provided references
1. 10.1089/ten.2006.12.277710.1089/ten.2006.12.2777 ↗
2. 10.1016/j.biomaterials.2010.04.03510.1016/j.biomaterials.2010.04.035 ↗
3. Mehrotra S. · Adv. Funct. Mater. · 2010 · 20 · 24710.1002/adfm.200901172 ↗
4. Mehrotra S. · Langmuir · 2010 · 26 · 1279410.1021/la101689z ↗
5. Capizzi R. L. · Invest. New Drugs · 1996 · 14 · 24910.1007/BF00194527 ↗
6. 10.1002/adfm.20060123710.1002/adfm.200601237 ↗
7. Patil S. · Neurosci. Lett. · 2005 · 384 · 28810.1016/j.neulet.2005.05.003 ↗
8. Manakova S. · Toxicol. In Vitro · 2003 · 17 · 36710.1016/S0887-2333(03)00024-9 ↗
9. Calderon-Martinez D. · J. Neurosci. Methods · 2002 · 114 · 110.1016/S0165-0270(01)00493-9 ↗
10. Plagemann P. G. W. · Cancer Res. · 1978 · 38 · 978
11. 10.1172/JCI11047210.1172/JCI110472 ↗
12. Young J. D. · Biosci. Rep. · 1983 · 3 · 30910.1007/BF01122895 ↗
13. 10.1016/0006-2952(82)90266-010.1016/0006-2952(82)90266-0 ↗
14. Fram R. J. · Pharmacol. Ther. · 1985 · 31 · 16510.1016/0163-7258(85)90021-X ↗
15. Dessi F. · J. Neurochem. · 1995 · 64 · 198010.1046/j.1471-4159.1995.64051980.x ↗
16. Geller H. M. · J. Neurochem. · 2001 · 78 · 26510.1046/j.1471-4159.2001.00395.x ↗
17. Wallace T. L. · J. Neurosci. · 1989 · 9 · 11510.1523/JNEUROSCI.09-01-00115.1989 ↗
18. Tomkins C. E. · J. Cell Sci. · 1994 · 107 · 149910.1242/jcs.107.6.1499 ↗
19. Keime-Guibert F. · J. Neurol. · 1998 · 245 · 69510.1007/s004150050271 ↗
20. Blanco M. D. · Biomaterials · 1998 · 19 · 86110.1016/S0142-9612(97)00247-0 ↗
21. Kwong Y. L. · Ann. Hematol. · 2009 · 88 · 19310.1007/s00277-008-0645-y ↗
22. Glantz M. J. · J. Clin. Oncol. · 1999 · 17 · 311010.1200/JCO.1999.17.10.3110 ↗
23. 10.1016/S0939-6411(03)00154-110.1016/S0939-6411(03)00154-1 ↗
24. Esposito E. · Int. J. Pharm. · 1995 · 117 · 15110.1016/0378-5173(94)00313-T ↗
25. 10.1002/(SICI)1097-0126(199909)48:9<843::AID-PI225>3.0.CO;2-810.1002/(SICI)1097-0126(199909)48:9<843::AID-PI225>3.0.CO;2-8 ↗
26. Bouhadir K. H. · J. Pharm. Sci. · 2000 · 89 · 91010.1002/1520-6017(200007)89:7<910::AID-JPS8>3.0.CO;2-# ↗
27. 10.1016/j.polymer.2008.01.02710.1016/j.polymer.2008.01.027 ↗
28. 10.1002/jbm.a.3053710.1002/jbm.a.30537 ↗
29. 10.1038/nbt1101-102910.1038/nbt1101-1029 ↗
30. 10.1016/j.jconrel.2007.03.00310.1016/j.jconrel.2007.03.003 ↗
31. Fatin-Rouge N. · J. Phys. Chem. B · 2003 · 107 · 1212610.1021/jp0303164 ↗
32. Liang S. M. · J. Control. Rel. · 2006 · 115 · 18910.1016/j.jconrel.2006.08.006 ↗
33. Gutenwik J. · AIChE J. · 2004 · 50 · 300610.1002/aic.10231 ↗
34. Maaloum M. · Electrophoresis · 1998 · 19 · 160610.1002/elps.1150191015 ↗
35. Pernodet N. · Electrophoresis · 1997 · 18 · 5510.1002/elps.1150180111 ↗
36. Xiong J. Y. · J. Phys. Chem. B · 2005 · 109 · 563810.1021/jp044473u ↗
37. Lynch I. · J. Phys. Chem. B · 2005 · 109 · 625710.1021/jp0502149 ↗
38. 10.1163/156856204236809510.1163/1568562042368095 ↗
39. 10.1016/j.biomaterials.2005.05.08310.1016/j.biomaterials.2005.05.083 ↗
40. 10.1002/adma.20050161210.1002/adma.200501612 ↗
41. 10.1163/15685620432279353910.1163/156856204322793539 ↗
42. 10.1126/science.277.5330.123210.1126/science.277.5330.1232 ↗
43. 10.1002/adma.20070174810.1002/adma.200701748 ↗
44. 10.1002/adma.20060011310.1002/adma.200600113 ↗
45. 10.1039/B600460C10.1039/B600460C ↗
46. 10.1002/adma.20040195110.1002/adma.200401951 ↗
47. 10.1163/15685620778103416010.1163/156856207781034160 ↗
48. 10.1021/nn700408z10.1021/nn700408z ↗
49. 10.1002/adfm.20040058810.1002/adfm.200400588 ↗
50. 10.1002/anie.20039029310.1002/anie.200390293 ↗
51. 10.1021/la047648010.1021/la0476480 ↗
52. 10.1073/pnas.060288410310.1073/pnas.0602884103 ↗
53. 10.1021/ma970048610.1021/ma9700486 ↗
54. 10.1002/marc.1997.03018060910.1002/marc.1997.030180609 ↗
55. 10.1021/ja002410t10.1021/ja002410t ↗
56. 10.1021/ma011346c10.1021/ma011346c ↗
57. 10.1021/la049777m10.1021/la049777m ↗
58. Mehrotra S. · Acta Biomater. · 2009 · 5 · 147410.1016/j.actbio.2009.01.004 ↗
59. Ono S. S. · Nano Lett. · 2006 · 6 · 59210.1021/nl0515504 ↗
60. Chandler L. J. · J. Neurochem. · 1993 · 60 · 157810.1111/j.1471-4159.1993.tb03326.x ↗
61. 10.1016/j.ejmech.2003.10.00510.1016/j.ejmech.2003.10.005 ↗
62. 10.1351/pac19976904079110.1351/pac199769040791 ↗
63. 10.1016/j.biomaterials.2005.06.03910.1016/j.biomaterials.2005.06.039 ↗
64. 10.1021/la981574910.1021/la9815749 ↗
65. 10.1021/la010848g10.1021/la010848g ↗
66. 10.1073/pnas.20248609910.1073/pnas.202486099 ↗
67. Ren K. F. · Biomaterials · 2006 · 27 · 115210.1016/j.biomaterials.2005.07.021 ↗
68. Patil S. · BMC Res. Notes · 2008 · 1 · 2010.1186/1756-0500-1-20 ↗
69. 10.1021/ma970610s10.1021/ma970610s ↗