Cytocompatibility and osteogenic differentiation of stem cells from human exfoliated deciduous teeth with cotton cellulose nanofibers for tissue engineering and regenerative medicine

Rafaella de S. S. Zanette; Leonara Fayer; Eduarda R. de Oliveira; Camila G. Almeida; Cauê R. Oliveira; Luiz F. C. de Oliveira; Carlos M. C. Maranduba; Érika C. Alvarenga; Humberto M. Brandão; Michele Munk

doi:10.1080/09205063.2021.2008787

journal article·2022-03-24

Cytocompatibility and osteogenic differentiation of stem cells from human exfoliated deciduous teeth with cotton cellulose nanofibers for tissue engineering and regenerative medicine

Rafaella de S. S. Zanette, Leonara Fayer, Eduarda R. de Oliveira, Camila G. Almeida, Cauê R. Oliveira, Luiz F. C. de Oliveira, Carlos M. C. Maranduba, Érika C. Alvarenga, Humberto M. Brandão, Michele Munk

Journal of Biomaterials Science, Polymer Edition· Volume 33· Issue 5

Publisher page

Source abstract

Abstract

Cellulose nanofibers (CNFs) are natural polymers with physical-chemical properties that make them very attractive for modulating stem cell differentiation, a crucial step in tissue engineering and regenerative medicine. Although cellulose is cytocompatible, when materials are in nanoscale, they become more reactive, needing to evaluate its potential toxic effect to ensure safe application. This study aimed to investigate the cytocompatibility of cotton CNF and its differentiation capacity induction on stem cells from human exfoliated deciduous teeth. First, the cotton CNF was characterized. Then, the cytocompatibility and the osteogenic differentiation induced by cotton CNF were examined. The results revealed that cotton CNFs have about 6-18 nm diameters, and the zeta potential was -10 mV. Despite gene expression alteration, the cotton CNF shows good cytocompatibility. The cotton CNF induced an increase in phosphatase alkaline activity and extracellular matrix mineralization. The results indicate that cotton CNF has good cytocompatibility and can promote cell differentiation without using chemical inducers, showing great potential as a new differentiation inductor for tissue engineering and regenerative medicine applications.

Source: openalex_inverted_index · Quality: baseline

Research context

Polymers Nanomaterials Biomaterials

OpenAlex topics: Electrospun Nanofibers in Biomedical Applications · Mesenchymal stem cell research · Tissue Engineering and Regenerative Medicine

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Laboratory of Nanobiotechnology and Nanotoxicology, Department of Biology, Federal University of Juiz de Fora, Juiz de Fora, Brazil

Laboratory of Applied Nanotechnology for Animal Production and Health, Brazilian Agricultural Research Corporation (EMBRAPA), Juiz de Fora, Brazil

National Laboratory of Nanotechnology for Agriculture, Embrapa Instrumentation, São Carlos, Brazil

Nucleus of Spectroscopy and Molecular Structure, Department of Chemistry, Federal University of Juiz de Fora, Juiz de Fora, Brazil

Laboratory of Human Genetics and Cell Therapy, Department of Biology, Federal University of Juiz de Fora, Juiz de Fora, Brazil

Department of Natural Sciences, Federal University of São João Del Rei, São João del Rei, Brazil

Funding

Fundação de Amparo à Pesquisa do Estado de Minas Gerais · Conselho Nacional de Desenvolvimento Científico e Tecnológico · Rede de Mineira de Pesquisa e Inovação para Bioengenharia de Nanossistemas · Rede de Nanotecnologia Aplicada ao Agronegócio · Coordenação de Aperfeiçoamento de Pessoal de Nível Superior · Pós-Graduação em Ciências Biológicas

References (84)

Show source-provided references

1. 10.1016/j.msec.2017.08.04310.1016/j.msec.2017.08.043 ↗

2. 10.1016/j.dental.2020.08.00610.1016/j.dental.2020.08.006 ↗

3. 10.1016/j.matlet.2018.11.01810.1016/j.matlet.2018.11.018 ↗

4. 10.1021/nn101373r10.1021/nn101373r ↗

5. 10.1039/C5TB02007G10.1039/C5TB02007G ↗

6. 10.1016/j.msec.2017.07.04210.1016/j.msec.2017.07.042 ↗

7. 10.1016/j.actbio.2019.07.00710.1016/j.actbio.2019.07.007 ↗

8. 10.3390/nano902016410.3390/nano9020164 ↗

9. 10.1016/j.chroma.2014.09.04310.1016/j.chroma.2014.09.043 ↗

10. 10.2147/IJN.S12431810.2147/IJN.S124318 ↗

11. 10.1016/j.ijbiomac.2016.12.09110.1016/j.ijbiomac.2016.12.091 ↗

12. 10.1016/j.colsurfb.2015.04.01410.1016/j.colsurfb.2015.04.014 ↗

13. 10.1016/j.colsurfb.2016.08.00810.1016/j.colsurfb.2016.08.008 ↗

14. 10.1080/09205063.2019.164662710.1080/09205063.2019.1646627 ↗

15. 10.1021/acs.biomac.8b0119410.1021/acs.biomac.8b01194 ↗

16. 10.1021/bm200865j10.1021/bm200865j ↗

17. 10.1088/0957-4484/24/7/07510310.1088/0957-4484/24/7/075103 ↗

18. 10.1016/j.nano.2016.01.00610.1016/j.nano.2016.01.006 ↗

19. 10.1007/s00204-018-2357-y10.1007/s00204-018-2357-y ↗

20. 10.1016/j.yrtph.2019.03.01610.1016/j.yrtph.2019.03.016 ↗

21. 10.1073/pnas.093763510010.1073/pnas.0937635100 ↗

22. 10.1111/j.1601-0825.2007.01396.x10.1111/j.1601-0825.2007.01396.x ↗

23. 10.1007/978-981-10-8471-3_6010.1007/978-981-10-8471-3_60 ↗

24. 10.1088/1748-605X/ab0a5210.1088/1748-605X/ab0a52 ↗

25. 10.1002/sctm.18-018210.1002/sctm.18-0182 ↗

26. 10.2147/CCIDE.S24567810.2147/CCIDE.S245678 ↗

27. 10.1155/2018/565378710.1155/2018/5653787 ↗

28. 10.1007/s10103-018-2637-z10.1007/s10103-018-2637-z ↗

29. 10.1016/S0022-2860(01)00706-210.1016/S0022-2860(01)00706-2 ↗

30. 10.1016/j.biomaterials.2017.01.02710.1016/j.biomaterials.2017.01.027 ↗

31. 10.1016/j.carbpol.2017.05.02710.1016/j.carbpol.2017.05.027 ↗

32. 10.1093/nar/30.9.e3610.1093/nar/30.9.e36 ↗

33. 10.1007/s10854-016-6106-910.1007/s10854-016-6106-9 ↗

34. 10.1007/s10570-014-0172-z10.1007/s10570-014-0172-z ↗

35. 10.1371/journal.pone.006575610.1371/journal.pone.0065756 ↗

36. 10.1016/S0378-5173(03)00364-810.1016/S0378-5173(03)00364-8 ↗

37. 10.3389/fchem.2018.0040910.3389/fchem.2018.00409 ↗

38. 10.1016/j.saa.2014.06.07110.1016/j.saa.2014.06.071 ↗

39. 10.1002/masy.20055050310.1002/masy.200550503 ↗

40. Ciolacu D · Cellul Chem Technol · 2011 · 45 · 13

41. 10.3390/ma709610510.3390/ma7096105 ↗

42. 10.1016/B978-0-08-100957-4.00001-210.1016/B978-0-08-100957-4.00001-2 ↗

43. 10.1371/journal.pone.006208710.1371/journal.pone.0062087 ↗

44. 10.1016/j.carbon.2015.12.02710.1016/j.carbon.2015.12.027 ↗

45. 10.1016/j.biomaterials.2014.05.02310.1016/j.biomaterials.2014.05.023 ↗

46. 10.1039/c3nr02148c10.1039/c3nr02148c ↗

47. 10.1002/jat.312810.1002/jat.3128 ↗

48. 10.4028/www.scientific.net/NHC.13.2610.4028/www.scientific.net/NHC.13.26 ↗

49. 10.3390/molecules2103036510.3390/molecules21030365 ↗

50. 10.1080/14686996.2016.123949910.1080/14686996.2016.1239499 ↗

51. 10.1007/s10495-012-0705-610.1007/s10495-012-0705-6 ↗

52. 10.1016/j.actbio.2011.06.05210.1016/j.actbio.2011.06.052 ↗

53. 10.1016/j.ijpharm.2016.06.11910.1016/j.ijpharm.2016.06.119 ↗

54. 10.1007/s00204-016-1701-310.1007/s00204-016-1701-3 ↗

55. 10.1016/j.tox.2018.05.00110.1016/j.tox.2018.05.001 ↗

56. 10.1007/s00204-012-0925-010.1007/s00204-012-0925-0 ↗

57. 10.2147/IJN.S14007110.2147/IJN.S140071 ↗

58. 10.1002/jat.138510.1002/jat.1385 ↗

59. 10.1007/s12274-014-0553-510.1007/s12274-014-0553-5 ↗

60. 10.1111/exd.1217810.1111/exd.12178 ↗

61. 10.3109/17435390.2015.107144310.3109/17435390.2015.1071443 ↗

62. 10.1016/j.msec.2016.05.10310.1016/j.msec.2016.05.103 ↗

63. 10.1007/s00204-013-1086-510.1007/s00204-013-1086-5 ↗

64. 10.1073/pnas.161640011310.1073/pnas.1616400113 ↗

65. 10.1007/s10565-014-9270-910.1007/s10565-014-9270-9 ↗

66. 10.2147/IJN.S13932610.2147/IJN.S139326 ↗

67. 10.1007/s00204-014-1448-710.1007/s00204-014-1448-7 ↗

68. 10.1016/j.tcb.2008.01.00710.1016/j.tcb.2008.01.007 ↗

69. 10.1016/j.colsurfb.2010.01.03310.1016/j.colsurfb.2010.01.033 ↗

70. 10.1038/emm.2003.5910.1038/emm.2003.59 ↗

71. 10.1083/jcb.20170205810.1083/jcb.201702058 ↗

72. 10.1016/j.jhazmat.2013.04.04610.1016/j.jhazmat.2013.04.046 ↗

73. 10.1080/01480545.2016.125595210.1080/01480545.2016.1255952 ↗

74. 10.1038/msb.2008.5910.1038/msb.2008.59 ↗

75. 10.1039/C7EN00704C10.1039/C7EN00704C ↗

76. Jafary F · Int J Organ Transplant Med · 2017 · 8 · 195

77. Hashemibeni B · Int J Prev Med · 2013 · 4 · 180

78. 10.1016/j.tiv.2013.12.00110.1016/j.tiv.2013.12.001 ↗

79. 10.1007/s10616-019-00353-y10.1007/s10616-019-00353-y ↗

80. 10.3390/ijms2020513510.3390/ijms20205135 ↗

81. 10.1016/j.carbpol.2017.08.00110.1016/j.carbpol.2017.08.001 ↗

82. 10.1002/adma.20170569410.1002/adma.201705694 ↗

83. 10.1155/2019/649523910.1155/2019/6495239 ↗

84. 10.1002/biot.20160045310.1002/biot.201600453 ↗