journal article·2010-01

Measurements of Movement and Diffusion Coefficients of Single Cells on Polymeric Surface from Image Analysis

Akon Higuchi, Ho Ling-Yi, Li Ya Huang, Hui Chen, Yi-Jen Chen, Qing-Dong Ling

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Abstract

Time-lapse digital images were taken every 30 s of PC12 cells cultured on polystyrene dishes, collagen-coated dishes and poly(L-lysine)-coated dishes in high-serum medium, low-serum medium and neurite outgrowth factor (NGF)-containing medium to investigate their diffusion coefficients (i.e., self-diffusion coefficients), D and specific movement (i.e., specific frequent movement) using image analysis and Fast Fourier Transform (FFT) analysis. D for these cells was found to fluctuate as a function of time, D varying between 0 and 0.08 microm(2)/s. The trend observed upon examination of average D values was: D in high-serum medium > or = D in low-serum medium > or = D in NGF-containing medium. Image analysis and FFT analysis of single cells cultured on polymeric dishes in these three media did not have any specific frequency of cell movement between 0 and 0.0167 Hz. The high diffusion coefficient and high amplitude of power spectra of PC12 cells in high-serum medium might be attributed to the high energy necessary for their continual suppression of the mitogen-activated protein kinase (MAPK) cascade and for them to maintain their undifferentiated state.
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Research context

OpenAlex topics: 3D Printing in Biomedical Research · Cellular Mechanics and Interactions · Microfluidic and Bio-sensing Technologies

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Affiliations

a Department of Chemical and Materials Engineering, National Central University, 300 Jhongda Road, Jhongli, Taoyuan 32001, Taiwan; Department of Reproduction, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan

b Department of Chemical and Materials Engineering, National Central University, 300 Jhongda Road, Jhongli, Taoyuan 32001, Taiwan

c Department of Chemical and Materials Engineering, National Central University, 300 Jhongda Road, Jhongli, Taoyuan 32001, Taiwan

d Department of Chemical and Materials Engineering, National Central University, 300 Jhongda Road, Jhongli, Taoyuan 32001, Taiwan

e National Yang-Ming University, 155, Sec. 2, Linong Street, Taipei 112, Taiwan; Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, 201 Section 2, Shih-Pai Road, Taipei 11217, Taiwan

f Cell Biology and Anatomy Laboratory, Cathy Medical Research Institute, Cathy General Hospital, 32 Ln 160, Jian-Cheng Road, Hsi-Chi City, Taipei 221, Taiwan; Institute of Systems Biology and Bioinformatics, National Central University, 300 Jhongda Road, Jhongli, Taoyuan 32001, Taiwan

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