Pluronic F-127 as a Cell Carrier for Bone Tissue Engineering
Source abstract
Abstract
The objective of this study is to report the effect of Pluronic F-127 on osteoblast viability and phenotype maintenance in vitro. MG-63 cells are suspended in Pluronic F-127, and MTT assay, alkaline phosphatase activity, prostaglandin E 2 production, collagen-I, and cyclo-oxygenase-2 expression are assessed up to 6 days. Pluronic F-127 leads to a significant decrease in osteoblast viability throughout the 6-day experiment, without altering osteoblast phenotype. The addition of platelet-rich plasma to the polymer/cell construct leads to increased cell survival. When supplemented with bioactive factors, Pluronic F-127 could potentially be used as a cell carrier in bone tissue engineering.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Periodontal Regeneration and Treatments · Bone Tissue Engineering Materials · Electrospun Nanofibers in Biomedical Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Orthopaedic Research Laboratory, Department of Orthopaedics, Centre hospitalier Sacré-Coeur, Montréal, Québec, Canada
Orthopaedic Research Laboratory, Department of Orthopaedics, Centre hospitalier Sacré-Coeur, Montréal, Québec, Canada, Department of Orthopaedics, Centre hospitalier Maisonneuve-Rosemont, Montréal, Québec, Canada
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