PHEMA Hydrogels Modified through the Grafting of Phosphate Groups by ATRP Support the Attachment and Growth of Human Corneal Epithelial Cells
Source abstract
Abstract
Converting the surface of poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogel into a cell-adhesive surface has been successfully achieved through a method based on atom transfer radical polymerization (ATRP) grafting. Following activation of the surface hydroxyl groups of PHEMA by bromination, surface-initiated ATRP of mono(2-methacryloyloxyethyl) phosphate (MMEP) was conducted in a methanol—water system with Cu(I)Br as catalyst at room temperature. The conversion of PHEMA hydroxyl groups to brominated isobutyryl groups and the occurrence of grafting of PMMEP were confirmed by infrared and X-ray photoelectron spectroscopies. Cell attachment experiments were conducted by culturing human corneal limbal epithelial cells on the PMMEP-grafted PHEMA, and on unmodified PHEMA and tissue culture plastic for comparison. The results showed that the grafted PMMEP was homogeneously distributed, and the phosphate groups appeared to significantly promote the attachment, spreading and growth of cells, at a level comparable to the tissue culture plastic.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Electrospun Nanofibers in Biomedical Applications · Hydrogels: synthesis, properties, applications · Corneal Surgery and Treatments
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Queensland Eye Institute, 41 Annerley Road, South Brisbane Queensland 4101, Australia,
Queensland Eye Institute, 41 Annerley Road, South Brisbane Queensland 4101, Australia
Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, Brisbane, Queensland 4072, Australia
Queensland Eye Institute, 41 Annerley Road, South Brisbane Queensland 4101, Australia, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, Brisbane, Queensland 4072, Australia, School of Physical and Chemical Sciences, Queensland University of Technology, 2 George Street, Brisbane, Queensland 4001, Australia
Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane Queensland 4006, Australia
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