Influence of scaffold composition over in vitro osteogenic differentiation of hBMSCs and in vivo inflammatory response
Source abstract
Abstract
To understand the role of chitosan in chitosan-poly(butylene succinate) scaffolds (50% wt), 50%, 25%, and 0% of chitosan were used to produce different scaffolds. These scaffolds were in vitro seeded and cultured with human bone marrow stromal cells in osteogenic conditions, revealing that higher percentage of chitosan showed enhanced cell viability over time, adhesion, proliferation, and osteogenic differentiation. Scaffolds were also implanted in cranial defects and iliac submuscular region in Wistar rats, and the results evidenced that chitosan-containing scaffolds displayed mild inflammatory response and good integration with surrounding tissues, showed by connective tissue colonization and the presence of new blood vessels. Scaffolds without chitosan-evidenced necrotic tissue in scaffolds’ interior, proving that chitosan exerts a positive effect over cell behavior and displays a milder host inflammatory response in vivo.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Graphene and Nanomaterials Applications · 3D Printing in Biomedical Research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal
ICVS/3B’s – PT Government Associate Laboratory, Braga/Guimarães, Portugal
Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Kırıkkale University, Kırıkkale, Turkey
Department of Polymer Engineering, Yalova University, Yalova, Turkey
CMAT – Department of Mathematics and Applications, Mathematical Research Centre, University of Minho, Campus de Azurém, Guimarães, Portugal
Chemical Engineering Department and Bioengineering Division, Hacettepe University, Beytepe, Ankara, Turkey
References (51)
Show source-provided references
1. 10.1126/science.849352910.1126/science.8493529 ↗
2. 10.1146/annurev.bioeng.1.1.1910.1146/annurev.bioeng.1.1.19 ↗
3. 10.1038/7944910.1038/79449 ↗
4. 10.1089/ten.teb.2010.070410.1089/ten.teb.2010.0704 ↗
5. Tomihata K · Biomaterials · 1997 · 18
6. 10.1128/AAC.34.10.201910.1128/AAC.34.10.2019 ↗
7. 10.1016/0002-9610(70)90175-310.1016/0002-9610(70)90175-3 ↗
8. 10.1002/jbm.127010.1002/jbm.1270 ↗
9. 10.1016/j.biomaterials.2005.03.01610.1016/j.biomaterials.2005.03.016 ↗
10. 10.1016/S0092-8674(00)80429-810.1016/S0092-8674(00)80429-8 ↗
11. 10.1002/1097-4636(20000915)51:4<586::AID-JBM6>3.0.CO;2-S10.1002/1097-4636(20000915)51:4<586::AID-JBM6>3.0.CO;2-S ↗
12. 10.1016/S0142-9612(03)00209-610.1016/S0142-9612(03)00209-6 ↗
13. 10.1016/j.biomaterials.2003.08.06810.1016/j.biomaterials.2003.08.068 ↗
14. Jiang T · Biomaterials · 2006 · 27
15. 10.1021/bm900623j10.1021/bm900623j ↗
16. 10.1177/03913988070300030510.1177/039139880703000305 ↗
17. 10.1016/j.ijom.2007.11.01410.1016/j.ijom.2007.11.014 ↗
18. 10.1002/jbm.a.3237610.1002/jbm.a.32376 ↗
19. 10.1016/j.biomaterials.2010.01.13110.1016/j.biomaterials.2010.01.131 ↗
20. 10.1007/s10856-005-4709-410.1007/s10856-005-4709-4 ↗
21. 10.1021/bm701268s10.1021/bm701268s ↗
22. 10.1016/j.msea.2005.04.05510.1016/j.msea.2005.04.055 ↗
23. 10.1002/jbm.a.3222110.1002/jbm.a.32221 ↗
24. 10.1089/ten.tea.2007.015310.1089/ten.tea.2007.0153 ↗
25. 10.1021/bm900010210.1021/bm9000102 ↗
26. 10.1002/term.39110.1002/term.391 ↗
27. 10.1016/S0142-9612(00)00102-210.1016/S0142-9612(00)00102-2 ↗
28. 10.1146/annurev.bioeng.3.1.30710.1146/annurev.bioeng.3.1.307 ↗
29. 10.1016/j.biomaterials.2005.10.02010.1016/j.biomaterials.2005.10.020 ↗
30. 10.1016/0009-8981(91)90021-410.1016/0009-8981(91)90021-4 ↗
31. Lian J · J Oral Implantol · 1993 · 19 · 95
32. 10.1034/j.1600-0501.2001.012002121.x10.1034/j.1600-0501.2001.012002121.x ↗
33. Anderson HC · Clin Orthop Relat Res · 1995 · 314 · 266
34. 10.1002/jbm.a.3214410.1002/jbm.a.32144 ↗
35. 10.1016/j.biomaterials.2009.12.04910.1016/j.biomaterials.2009.12.049 ↗
36. 10.1177/1045441192003003050110.1177/10454411920030030501 ↗
37. 10.1634/stemcells.20-3-23010.1634/stemcells.20-3-230 ↗
38. 10.1359/JBMR.04111810.1359/JBMR.041118 ↗
39. 10.1016/j.bbrc.2006.01.09210.1016/j.bbrc.2006.01.092 ↗
40. 10.2174/15748880778169626710.2174/157488807781696267 ↗
41. 10.1002/(SICI)1097-4644(1998)72:30/31+<92::AID-JCB13>3.0.CO;2-A10.1002/(SICI)1097-4644(1998)72:30/31+<92::AID-JCB13>3.0.CO;2-A ↗
42. 10.1002/(SICI)1097-4636(199901)44:1<98::AID-JBM11>3.0.CO;2-P10.1002/(SICI)1097-4636(199901)44:1<98::AID-JBM11>3.0.CO;2-P ↗
43. 10.1101/gad.127620510.1101/gad.1276205 ↗
44. 10.1172/JCI1041310.1172/JCI10413 ↗
45. 10.1016/1054-8807(93)90045-410.1016/1054-8807(93)90045-4 ↗
46. 10.1146/annurev.matsci.31.1.8110.1146/annurev.matsci.31.1.81 ↗
47. 10.1016/S0169-409X(98)00023-410.1016/S0169-409X(98)00023-4 ↗
48. 10.1097/00002480-198804000-0000510.1097/00002480-198804000-00005 ↗
49. Kumar V · 2010
50. 10.1016/S0144-8617(00)00326-X10.1016/S0144-8617(00)00326-X ↗
51. 10.1292/jvms.56.121510.1292/jvms.56.1215 ↗