A Covalent Method of Gentamicin Bonding to Silica Supports
Source abstract
Abstract
Results of a novel method of covalent bonding of an antibiotic (gentamicin) to silica bead supports are shown. Gentamicin was immobilized to four types of matrix: silica gel and porous glass beads activated by either silanization (APTES) or by adhesively bound keratin (with immobilization yield ranging from 36.5 to 91%). Gentamicin was immobilized to the supports after opening its carbohydrate ring in the molecule. This method of gentamicin activation before the immobilization process did not inhibit its antibiotic activity. The four gentamicin-containing immobilized preparations were stable, meaning that they did not release the antibiotic into the solution during the 30 days of incubation, not even during shaking experiments.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Orthopedic Infections and Treatments · Antimicrobial agents and applications · Protein purification and stability
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Biochemistry Maria Curie-Sk&unknown;odowska University 20-031 Lublin, Poland
Division of General and Vascular Surgery Municipal Hospital 35-241 Rzeszów, Poland
References (23)
Show source-provided references
1. 10.1016/S0142-9612(00)00313-610.1016/S0142-9612(00)00313-6 ↗
2. 2. Gelabert H.A. and Colburn M.D. (1992). Development and Results of Antibiotic Impregnated Vascular Grafts In: Moore W.S. and Gelabert H.A. (eds) Antibiotic Impregnated Vascular Grafts pp. 88–100 Medical Intelligence Unit Austin .
3. 10.1016/S0142-9612(98)00164-110.1016/S0142-9612(98)00164-1 ↗
4. 10.1111/j.1445-2197.1991.tb00258.x10.1111/j.1445-2197.1991.tb00258.x ↗
5. 5. Ashton T.R. Cunningham J.D. Paton D. and Maini R. (1990). Antibiotic Loading of Vascular Grafts In: Proc. 16th Annual Meeting of the Society for Biomaterials Charleston South Carolina 20–23 May 13: 235–239 .
6. 6. Haverich, A., Hirt, S., Karak, M., Sialari, F. and Wahling, H. (1998). Prevention of Graft Infection by Bonding Gentamicin to Dacron Prostheses , J. Vasc. Surg., 15: 187–193 .
7. 10.1016/0022-4804(91)90020-M10.1016/0022-4804(91)90020-M ↗
8. 10.1016/S0950-821X(05)80896-510.1016/S0950-821X(05)80896-5 ↗
9. 10.1177/09672109980060030910.1177/096721099800600309 ↗
10. 10. British Pharmacopoeia (1998). HMSO London 1: 302.
11. 10.1016/S0076-6879(76)44012-010.1016/S0076-6879(76)44012-0 ↗
12. 10.1016/0006-291X(84)90710-110.1016/0006-291X(84)90710-1 ↗
13. 10.1016/S0378-5173(00)00520-210.1016/S0378-5173(00)00520-2 ↗
14. 14. European Pharmacopoeia (1997). 3rd edn 2.7.2. Microbiological Assay of Antibiotics European Department for the Quality of Medicines Strasbourg .
15. 15. Ginalska G. &unknown;obarzewski J. Cho N.S. Park J. Jaszek M. and Leonowicz A. (1999b). Keratin as a Matrix for Laccase Immobilization In: Cho N.S. (ed.) Proc. of ’99 International Conference on New Horizon of Bioscience in Forest Products Field pp. 164–174 Chungbuk National University Cheongju Korea.
16. 10.1006/jsre.1995.101710.1006/jsre.1995.1017 ↗
17. 10.1016/S0142-9612(02)00254-510.1016/S0142-9612(02)00254-5 ↗
18. 10.1016/S0142-9612(02)00614-210.1016/S0142-9612(02)00614-2 ↗
19. 10.1016/S0142-9612(97)00237-810.1016/S0142-9612(97)00237-8 ↗
20. 10.1016/S0142-9612(02)00066-210.1016/S0142-9612(02)00066-2 ↗
21. 10.1016/S0960-894X(97)10175-510.1016/S0960-894X(97)10175-5 ↗
22. 10.1016/0142-9612(96)87654-X10.1016/0142-9612(96)87654-X ↗
23. 10.1016/0142-9612(95)99636-Z10.1016/0142-9612(95)99636-Z ↗