Surface Treatment of Pure and PEG-4000 Blended Fibroin Films and their Characterizations as Matrices for in vitro Fibroblast Culture
Source abstract
Abstract
This study reports the effects of treatment with various concentrations of organic solvents for varying time points on matrices of fibroin, a silk protein isolated from the mulberry silkworm, Bombyx mori, which in native form has been extensively used in tissue engineering. Treatment of pure fibroin as well as polyethylene glycol- blended films with 90% organic solvent for 60 min induces optimal surface hydrophobicity and maximum conversion of the secondary structure from random coil to β sheet. Long-term cell viability studies reveal that methanol and isopropanol-treated pure and blended films support cell adhesion, proliferation, and viability.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Silk-based biomaterials and applications · Electrospun Nanofibers in Biomedical Applications · Polymer Surface Interaction Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Biotechnology, Indian Institute of Technology Kharagpur-721302, India
Department of Biotechnology, Indian Institute of Technology Kharagpur-721302, India,
References (43)
Show source-provided references
1. Lee, Y. · Bulletin · 1999
2. 10.1271/bbb.66.271510.1271/bbb.66.2715 ↗
3. 10.1016/0020-1790(77)90026-910.1016/0020-1790(77)90026-9 ↗
4. Tamura, T. and Kubota, T. (1988). A determination of molecular weight of fibroin polypeptides in the saturniid silkworms, Antheraea yamamai, Antheraea pernyi and Philosamia ricini by SDS-PAGE. In: Akai, H. (ed.), Wild Silkmoth '88. National Institute of Sericulture and Entomological Science, Tsukuba , Japan, pp. 67-72.
5. 10.1016/0020-1790(88)90021-210.1016/0020-1790(88)90021-2 ↗
6. 10.1016/0142-9612(90)90100-510.1016/0142-9612(90)90100-5 ↗
7. 10.1016/S0142-9612(03)00633-110.1016/S0142-9612(03)00633-1 ↗
8. 10.1016/j.biomaterials.2003.08.04510.1016/j.biomaterials.2003.08.045 ↗
9. 10.1016/S0142-9612(03)00619-710.1016/S0142-9612(03)00619-7 ↗
10. 10.1016/S0142-9612(02)00417-910.1016/S0142-9612(02)00417-9 ↗
11. 10.1021/ma00203a01710.1021/ma00203a017 ↗
12. 10.1007/s00339010098410.1007/s003390100984 ↗
13. 10.1002/polb.1995.09033140210.1002/polb.1995.090331402 ↗
14. 10.1016/S0040-6090(03)00460-710.1016/S0040-6090(03)00460-7 ↗
15. 10.1016/j.apsusc.2004.09.16710.1016/j.apsusc.2004.09.167 ↗
16. 10.1016/S0142-9612(01)00287-310.1016/S0142-9612(01)00287-3 ↗
17. 10.1016/j.biomaterials.2003.10.06210.1016/j.biomaterials.2003.10.062 ↗
18. 10.1016/S0144-8617(99)00088-010.1016/S0144-8617(99)00088-0 ↗
19. 10.1016/S0032-3861(96)00665-910.1016/S0032-3861(96)00665-9 ↗
20. 10.1016/S0142-9612(96)00137-810.1016/S0142-9612(96)00137-8 ↗
21. 10.1016/S0168-3659(02)00181-510.1016/S0168-3659(02)00181-5 ↗
22. 10.1002/1097-4636(200101)54:1<139::AID-JBM17>3.0.CO;2-710.1002/1097-4636(200101)54:1<139::AID-JBM17>3.0.CO;2-7 ↗
23. 10.1016/S0032-3861(01)00104-510.1016/S0032-3861(01)00104-5 ↗
24. 10.1016/j.polymdegradstab.2006.02.00810.1016/j.polymdegradstab.2006.02.008 ↗
25. Callister Jr, W.D. (1997). In Materials Science and Engineering: An Introduction , 4th edn, pp. 466-472, John Wiley & Sons Inc., New York.
26. 10.1002/bip.1042310.1002/bip.10423 ↗
27. 10.1002/1097-0282(200012)54:7<578::AID-BIP100>3.0.CO;2-210.1002/1097-0282(200012)54:7<578::AID-BIP100>3.0.CO;2-2 ↗
28. 10.1128/AEM.69.11.6777-6784.200310.1128/AEM.69.11.6777-6784.2003 ↗
29. 10.1016/S0167-4838(01)00242-410.1016/S0167-4838(01)00242-4 ↗
30. 10.1016/S0006-3495(95)79971-410.1016/S0006-3495(95)79971-4 ↗
31. 10.1016/S0301-4622(00)00213-110.1016/S0301-4622(00)00213-1 ↗
32. 10.1002/prot.2141410.1002/prot.21414 ↗
33. 10.1016/j.msec.2006.05.01710.1016/j.msec.2006.05.017 ↗
34. 10.1021/bm034384810.1021/bm0343848 ↗
35. 10.1016/S0049-3848(01)00254-710.1016/S0049-3848(01)00254-7 ↗
36. 10.1016/j.cca.2006.06.00310.1016/j.cca.2006.06.003 ↗
37. 10.1016/0142-9612(96)85758-910.1016/0142-9612(96)85758-9 ↗
38. 10.1016/S0142-9612(96)00137-810.1016/S0142-9612(96)00137-8 ↗
39. 10.1002/(SICI)1097-4636(19980305)39:3<351::AID-JBM2>3.0.CO;2-I10.1002/(SICI)1097-4636(19980305)39:3<351::AID-JBM2>3.0.CO;2-I ↗
40. 10.1002/jbm.82029100810.1002/jbm.820291008 ↗
41. 10.1002/(SICI)1097-4636(19990905)46:3<382::AID-JBM11>3.0.CO;2-R10.1002/(SICI)1097-4636(19990905)46:3<382::AID-JBM11>3.0.CO;2-R ↗
42. 10.1016/0003-9861(89)90401-310.1016/0003-9861(89)90401-3 ↗
43. 10.1016/0041-008X(85)90281-910.1016/0041-008X(85)90281-9 ↗