Tracheal reconstruction with pedicled tandem grafts engineered by a radial stretch bioreactor
Source abstract
Abstract
The engineering of tracheal substitutes is pivotal in improving tracheal reconstruction. In this study, we aimed to investigate the effects of biomechanical stimulation on tissue engineering tracheal cartilage by mimicking the trachea motion through a novel radial stretching bioreactor, which enables to dynamically change the diameter of the hollow cylindrical implants. Applying our bioreactor, we demonstrated that chondrocytes seeded on the surface of Poly (ε-caprolactone) scaffold respond to mechanical stimulation by improvement of infiltration into implants and upregulation of cartilage-specific genes. Further, the mechanical stimulation enhanced the accumulation of cartilage neo-tissues and cartilage-specific extracellular macromolecules in the muscle flap-remodeled implants and reconstructed trachea. Nevertheless, the invasion of fibrous tissues in the reconstructed trachea was suppressed upon mechanical loading.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Tracheal and airway disorders · Tissue Engineering and Regenerative Medicine · Reconstructive Surgery and Microvascular Techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Division of Reconstructive Microsurgery, Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital Linkou Main Branch, Taoyuan, Taiwan
Center for Tissue Engineering, Chang Gung Memorial Hospital Linkou Main Branch, Taoyuan, Taiwan
Division of Thoracic Surgery, Chang Gung Memorial Hospital Linkou Main Branch, Taoyuan, Taiwan
Division of Pediatric Neurosurgery, Chang Gung Children's Hospital, Chang Gung Memorial Hospital Linkou Main Branch, Taoyuan, Taiwan
Center of Lymphedema Microsurgery, Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital Linkou Main Branch, Taoyuan, Taiwan
Center for Biomedical Engineering, College of Engineering, Chang Gung University, Taoyuan, Taiwan
Funding
Chang Gung Memorial Hospital, Linkou
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