Immunogenicity of Protein-N-(2-Hydroxypropyl)methacrylamide Copolymer Conjugates in A/J and B10 Mice
Source abstract
Abstract
Two proteins (model targeting residues) human immunoglobulin fraction (IgG) and human transferrin have been conjugated to N-(2-hydroxy propyl)methacrylamide (HPMA) copolymer and the antibody titer elicited, after subcutaneous or intraperitoneal administration to A/J and B10 mice of free and conjugated protein, was measured using the ELISA technique. The measured IgG titer against protein-HPMA copolymer conjugates was always higher than the IgM titer. Also, the titer (IgG) measured against native protein was up to 250-fold greater than that raised against protein-HPMA copolymer conjugates. This reduction in antibody titer against conjugate had a limited de pendence on its molecular weight.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Protein purification and stability · Molecular Biology Techniques and Applications · Immunotoxicology and immune responses
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Cancer Research Campaign Laboratory Department of Biological Sciences University of Keele Keele, Staffs. ST5 5BG, England
Institute of Microbiology Czechoslovak Academy of Sciences Videnska 1083, 142 20 Prague 4
Institute of Macromolecular Chemistry Czechoslovak Academy of Sciences 162 06 Prague 6, Czechoslovakia
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