Nonradioisotope Assay to Examine Adaptability of Nonnatural Amino Acids to the Active Center of Ribosomal a Site

A puromycin analog was synthesized and used for assessing the adaptability of the nonnatural amino acid to the active center of reticulocytic ribosome by competitive protein synthesis inhibition using a nonradioisotope procedure. Adaptability of benzyl L-glutamate was assessed by the amino acid-coupled puromycin analog. The puromycin analog inhibited in vitro protein synthesis by the ribosome indicating that chemical modification of side groups by benzyl group did not significantly affect the adaptability of amino acids.