DataVXJournal of Bioactive and Compatible PolymersPaper
journal article·2011-09

Preparation of hydrophilic poly(3-hydroxybutyrate) macroporous scaffolds through enzyme-mediated modifications

Daniel Guzmán, Harald Kirsebom, Carlos Solano, Jorge Quillaguamán, Rajni Hatti-Kaul

Journal of Bioactive and Compatible Polymers· Volume 26· Issue 5
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Source abstract

Abstract

Scaffolds made from poly(3-hydroxybutyrate) were prepared by thermally induced phase separation from solutions in dioxane at temperatures above (23°C) or below the freezing point of the solvent. At 23°C, gelation occured with nano-fibrous network formation. The scaffolds prepared at —12°C and —25°C exhibited a highly porous morphology, with pores in the range of 3—25 μm, caused by freezing of dioxane. The macroporous scaffolds, prepared at —12°C, were treated with lipase to generate functional groups to which gelatin and glucosamine, respectively, were chemically coupled. The modified scaffolds had lower molecular weight, higher water content, lower melting temperature, and enthalpy. Cultivation of human embryonic fibroblasts on the macroporous scaffolds confirmed that the cells proliferated and adhered to the materials.
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Research context

OpenAlex topics: biodegradable polymer synthesis and properties · Electrospun Nanofibers in Biomedical Applications · Microplastics and Plastic Pollution

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden, Centro de Biotecnología, Facultad de Ciencias y Tecnología, Universidad Mayor de San Simón, Cochabamba, Bolivia

Department of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden

Department of Organic Chemistry, Lund University, SE-221 00 Lund, Sweden

Centro de Biotecnología, Facultad de Ciencias y Tecnología, Universidad Mayor de San Simón, Cochabamba, Bolivia

Department of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden,

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