DataVXAdvanced BiologyPaper
journal article·2024-12

Electroacupuncture Slows Experimental Myopia Progression by Improving Retinal Mitochondrial Function: A Study Based on Single‐Cell RNA Sequencing

Jiawen Hao, Yunxiao Xie, Huixia Wei, Zhaohui Yang, Ruixue Zhang, Zhongyu Ma, Miao Zhang, Xiaoshi Du, Xuewei Yin, Jinpeng Liu, Bo Bao, Hongsheng Bi, Dadong Guo

Advanced Biology· Volume 8· Issue 12
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Source abstract

Abstract

Abstract This study aimed to establish a complete atlas of retinal cells in lens‐induced myopia (LIM) and electroacupuncture (EA) intervention by single‐cell RNA sequencing (scRNA‐seq) and to explore the potential mechanism of EA in improving experimental myopia progression in guinea pigs. scRNA‐seq is used to assess changes in individual cellular gene levels in the retina of LIM‐ and EA‐treated guinea pigs. In addition, the role of EA in slowing myopia progression by improving retinal mitochondrial function is further investigated. scRNA‐seq identified ten cell clusters in the retina of LIM and EA guinea pigs and mitochondrial respiratory chain‐related genes in Cones and Muller‐glia cells—Cytochrome oxidase subunit III (COX3), NADH dehydrogenase subunit 4 (ND4), and NADH dehydrogenase subunit 2 (ND2) are closely related to lens‐induced myopia. A comprehensive atlas in the retina of LIM and EA guinea pigs at a single‐cell level is established, and the positive role of EA in improving retinal mitochondrial function to slow the experimental myopia progression in guinea pigs is revealed.
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Research context

OpenAlex topics: Ophthalmology and Visual Impairment Studies · Corneal surgery and disorders · Retinal Diseases and Treatments

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Shandong University of Traditional Chinese Medicine Jinan 250002 China

Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases Shandong Academy of Eye Disease Prevention and Therapy Shandong Provincial Clinical Research Center of Ophthalmology and Children Visual Impairment Prevention and Control Shandong Engineering Technology Research Center of Visual Intelligence Shandong Academy of Health and Myopia Prevention and Control of Children and Adolescents Jinan 250002 China

Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine Jinan 250002 China

Medical College of Optometry and Ophthalmology Shandong University of Traditional Chinese Medicine Jinan 250002 China

Funding

National Key Research and Development Program of China

References (41)

Show source-provided references
1. 10.1016/j.ophtha.2016.01.00610.1016/j.ophtha.2016.01.006 ↗
2. 10.1111/opo.1320610.1111/opo.13206 ↗
3. 10.1371/journal.pone.017592110.1371/journal.pone.0175921 ↗
4. 10.3390/ijerph2001087510.3390/ijerph20010875 ↗
5. 10.1016/j.exer.2019.10777810.1016/j.exer.2019.107778 ↗
6. 10.1016/j.preteyeres.2024.10124610.1016/j.preteyeres.2024.101246 ↗
7. 10.1097/IAE.000000000000148910.1097/IAE.0000000000001489 ↗
8. YZH H. J. W. · Recent Adv. Ophthalmol. · 2023 · 43 · 940
9. 10.1186/s12974-022-02621-910.1186/s12974-022-02621-9 ↗
10. 10.2337/db20-083910.2337/db20-0839 ↗
11. 10.1167/iovs.64.11.210.1167/iovs.64.11.2 ↗
12. 10.1093/hmg/ddad00710.1093/hmg/ddad007 ↗
13. 10.1016/j.celrep.2020.01.00710.1016/j.celrep.2020.01.007 ↗
14. 10.7554/eLife.8650710.7554/eLife.86507 ↗
15. 10.1001/jamaoncol.2021.031010.1001/jamaoncol.2021.0310 ↗
16. 10.1038/d41586-021-02714-010.1038/d41586-021-02714-0 ↗
17. 10.3389/fninf.2020.57976910.3389/fninf.2020.579769 ↗
18. 10.1155/2022/328658310.1155/2022/3286583 ↗
19. 10.18240/ijo.2023.12.0510.18240/ijo.2023.12.05 ↗
20. 10.1111/j.1444-0938.2001.tb05037.x10.1111/j.1444-0938.2001.tb05037.x ↗
21. 10.1038/s41592-019-0619-010.1038/s41592-019-0619-0 ↗
22. 10.1016/j.neuroscience.2014.12.02210.1016/j.neuroscience.2014.12.022 ↗
23. 10.3748/wjg.v13.i36.487310.3748/wjg.v13.i36.4873 ↗
24. 10.1167/iovs.63.11.510.1167/iovs.63.11.5 ↗
25. 10.1016/j.visres.2006.01.02710.1016/j.visres.2006.01.027 ↗
26. 10.1016/j.exer.2020.10833910.1016/j.exer.2020.108339 ↗
27. 10.1006/meth.2001.126210.1006/meth.2001.1262 ↗
28. 10.1073/pnas.172144311510.1073/pnas.1721443115 ↗
29. 10.1007/s00438-023-02036-y10.1007/s00438-023-02036-y ↗
30. M. Y. Z. H. Y. M. J. IJO 2023 23 1449[Chinese].
31. 10.1042/bse047000110.1042/bse0470001 ↗
32. 10.1007/s10545-006-0362-410.1007/s10545-006-0362-4 ↗
33. 10.1007/s00438-023-02036-y10.1007/s00438-023-02036-y ↗
34. 10.1167/iovs.16-2094110.1167/iovs.16-20941 ↗
35. 10.1155/2021/472871410.1155/2021/4728714 ↗
36. 10.1097/CM9.000000000000303210.1097/CM9.0000000000003032 ↗
37. 10.1136/jmedgenet-2020-10722010.1136/jmedgenet-2020-107220 ↗
38. 10.3389/fncel.2020.0012410.3389/fncel.2020.00124 ↗
39. 10.1111/cpr.1341210.1111/cpr.13412 ↗
40. Yu J. · J. South. Med. Uni. · 2021 · 41 · 1087
41. 10.1073/pnas.172144311510.1073/pnas.1721443115 ↗