journal article·2010-09

Chitosan Membrane with Surface-bonded Growth Factor in Guided Tissue Regeneration Applications

Chih-Hao Chang, Ching-Ting Tsao, Ken-Yu Chang, Jaw-Ling Wang, Tai-Horng Young, Jin-Lin Han, Kuo-Huang Hsieh

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Abstract

The potential of surface covalently bonded rhBMP-2 biodegradable chitosan membrane was examined for guided tissue regeneration (GTR) applications. A chitosan surface-bonded rhBMP-2 membrane was produced via amide bond formation between chitosan and rhBMP-2 using EDC/NHS as the catalyst. The chitosan surface-bonded rhBMP-2 membrane retained more than 70% of the initial rhBMP-2 after 4 weeks of incubation, whereas the chitosan surface-adsorbed rhBMP-2 membrane retained only 30%. The surface-bonded rhBMP-2 did not denature, but expressed sustained biological activity, such as osteoblast cell adhesion, proliferation, and differentiation. X-ray images and histology of an in vivo segmental bone defect rabbit model showed that the chitosan surface-bonded rhBMP-2 membrane induced new bone formation. The chitosan surface-bonded rhBMP-2 membrane has the potential as a bioactive material for GTR.
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Research context

OpenAlex topics: Bone Tissue Engineering Materials · Electrospun Nanofibers in Biomedical Applications · Polymer Surface Interaction Studies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Biomedical Engineering, College of Engineering and College of Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Da-an Dist., Taipei City 10617, Taiwan, Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, No. 1, Jen-ai Road Zhong-zheng Dist., Taipei City 10051, Taiwan

Institute of Polymer Science and Engineering, National Taiwan University No.1, Sec. 4, Roosevelt Road, Da-an Dist., Taipei City 10617, Taiwan

Department of Chemical Engineering, National Taiwan University, No.1 Sec. 4, Roosevelt Road, Da-an Dist., Taipei City 10617, Taiwan

Institute of Biomedical Engineering, College of Engineering and College of Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Da-an Dist., Taipei City 10617, Taiwan

Department of Chemical and Materials Engineering National Ilan University, No.1, Sec. 1, Shennong Road, Ilan City Ilan County 26047, Taiwan

Institute of Polymer Science and Engineering, National Taiwan University No.1, Sec. 4, Roosevelt Road, Da-an Dist., Taipei City 10617, Taiwan, Department of Chemical Engineering, National Taiwan University, No.1 Sec. 4, Roosevelt Road, Da-an Dist., Taipei City 10617, Taiwan,

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