Preparation and evaluation of PCLA2575 membranes loaded ornidazole in vitro

The aim of this study was to evaluate the properties of the sustained release and antibacterial activity of the ornidazole-drug-loaded membranes using poly[(ethylene glycol)-caprolactone-lactide] (PCLA2575) as membrane material. Ornidazole-loaded membranes were prepared by solvent casting method with the proportion of 5 wt%, 8 wt%, and 10 wt%, respectively. In vitro drug release properties were determined by ultraviolet spectrophotometric method. The antibacterial activities against Streptococcus mutans and Fusobacterium nucleatum in vitro were observed on solid culture medium. The membrane had the high drug loadings and slow-release performance. Drug release time was shortened with the increase in the content of ornidazole, but all of them can achieve more than 7 days. The membrane had strong inhibitory effect on both S. mutans and F. nucleatum. As drug content increased, the antibacterial activities also increased. The membrane had better inhibitory effect on F. nucleatum than S. mutans. Therefore, the ornidazole drug-loaded membrane is expected to be used for the treatment of periodontal disease because of the obvious effect of periodontal pathogens inhibition and good sustained-release performance.