Behavior of human cells in contact with a poly( d , l -lactic acid) porous matrix after calcification using phosphatidylserine

As part of a strategy aimed at improving bioresorbable scaffolds for the engineering of bony tissues, a route to deposit calcium phosphate onto surfaces of poly(dl-lactic acid)–based porous matrices was investigated. Porosity was generated using the NaCl-leaching technique. Calcification was achieved after deposition of phosphatidylserine, a nucleating agent of natural origin, onto pore surfaces, followed by incubation of the phospholipid-coated matrix in a pH 6.5 aqueous medium consisted of 3.5 mmol CaCl ₂ and 2.6 mmol KH ₂ PO ₄ for 3 days. Calcified matrices were noncytotoxic according to the ISO10993-5 standard test and exhibited low inflammatory potential. To compare responses of human cells of different types, human osteogenic bone marrow cells from the femoral head, human chondrocytes from femoral cartilage collected after hip surgery, and human vascular endothelial cells isolated from an umbilical cord were allowed to grow in the presence of the calcified matrices in vitro. Articular chondrocytes adhered to and grew on the calcified matrices up to colony formation. In contrast, the other two types of cells attached and proliferated for approximately 3 days and then detached. These different cell behaviors are discussed with respect to the nature of the cells and to the release of calcium ions from the coating.