Immobilization of vascular endothelial growth factor onto semi-resorbable membrane for enhancing angiogenesis in guided bone regeneration
Source abstract
Abstract
This study incorporated Vascular Endothelial Growth Factor (VEGF) and polydopamine (PDA) into a Semi-resorbable membrane (SRM) to control the release profile of VEGF, thereby enhancing timely and sufficient vascularization in guided bone regeneration. Dopamine (DA) was optimized and selected using Scanning Electron Microscope (SEM), Water Contact Angle (WCA), and Fourier Transform Infrared Spectroscopy (FTIR). After PDA coating, VEGF-immobilized SRMs were prepared at different concentrations (0.1, 0.3, 0.5, and 1 µg). Then, the released profile of VEGF 1 µg was evaluated with the Bradford Assay at specific time points up to 1 week. The degradation rate (%) was assessed hydrolytically and enzymatically up to 180 days. Cell Proliferation of fibroblasts and Human umbilical vein endothelial cells (HUVECs) was examined with PrestoBlue Assay, at 1-, 3-, 7-, and 10-day intervals. From SEM, WCA, and FTIR, polydopamine 1 mg/ml for 1 h. was selected for coating and VEGF immobilization. The released profile of VEGF showed a sharp increase up to 6 h., then stable and slightly decreased on Day 2, and slowly released and stable until Day 7. The VEGF-immobilized SRM was slowly degraded by SBF (18.04% ± 2.13%) and lysozyme (24.44% ± 2.93%) in 180 days. In the cell proliferation assay, all experimental groups showed increased proliferation of fibroblasts and HUVECs at all concentrations. The maximum amount of VEGF (0.5 μg/ml) gained the best result, but no statistical significance between 0.5 and 0.3 μg/ml on Day 10. VEGF-immobilized SRMs exhibited biocompatibility with fibroblasts and HUVECs. They effectively released VEGF during the first week, corresponding to the early phase of bone healing, demonstrating potential as bioactive membranes for angiogenesis.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Periodontal Regeneration and Treatments · Polymer Surface Interaction Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Oral and Maxillofacial Surgery Section, Faculty of Dentistry, Prince of Songkla University, Hat yai, Songkhla, Thailand
Institute of Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat yai, Songkhla, Thailand
Funding
prince of songkla university · Faculty of Dentistry, Prince of Songkla University
References (48)
Show source-provided references
1. Novosel EC Kleinhans C Kluger PJ. Vascularization is the key challenge in tissue engineering. Adv Drug Deliv Rev 2011; 63: 300–311. https://doi.org/10.1016/j.addr.2011.03.00410.1016/j.addr.2011.03.004 ↗
2. Certelli A Valente P Uccelli A et al Robust angiogenesis and arteriogenesis in the skin of diabetic mice by transient delivery of engineered VEGF and PDGF-BB proteins in fibrin hydrogels. Front Bioeng Biotechnol 2021; 9: 688467. https://doi.org/10.3389/fbioe.2021.68846710.3389/fbioe.2021.688467 ↗
3. Kneser U Polykandriotis E Ohnolz J et al Engineering of vascularized transplantable bone tissues: induction of axial vascularization in an osteoconductive matrix using an arteriovenous loop. Tissue Eng 2006; 12: 1721–1731. https://doi.org/10.1089/ten.2006.12.172110.1089/ten.2006.12.1721 ↗
4. Silva AS Santos LF Mendes MC et al Multi-layer pre-vascularized magnetic cell sheets for bone regeneration. Biomaterials 2020; 231: 119664. https://doi.org/10.1016/j.biomaterials.2019.11966410.1016/j.biomaterials.2019.119664 ↗
5. 10.1016/j.jmbbm.2021.10484110.1016/j.jmbbm.2021.104841 ↗
6. Fishero BA Kohli N Das A et al Current concepts of bone tissue engineering for craniofacial bone defect repair. Craniomaxillofac Trauma Reconstr 2015; 8: 23–30. https://doi.org/10.1055/s-0034-139372410.1055/s-0034-1393724 ↗
7. Turri A Elgali I Vazirisani F et al Guided bone regeneration is promoted by the molecular events in the membrane compartment. Biomaterials 2016; 84: 167–183. https://doi.org/10.1016/j.biomaterials.2016.01.03410.1016/j.biomaterials.2016.01.034 ↗
8. Lee YJ Lee JH Cho HJ et al Electrospun fibers immobilized with bone-forming peptide-1 derived from BMP7 for guided bone regeneration. Biomaterials 2013; 34: 5059–5069. https://doi.org/10.1016/j.biomaterials.2013.03.05110.1016/j.biomaterials.2013.03.051 ↗
9. Masson-Meyers DS Tabatabaei F Steinhaus L et al Development of fibroblast/endothelial cell-seeded collagen scaffolds for in vitro prevascularization. J Biomed Mater Res B Appl Biomater 2023; 111: 633–645. https://doi.org/10.1002/jbm.b.3518210.1002/jbm.b.35182 ↗
10. 10.1038/nm0603-66910.1038/nm0603-669 ↗
11. Schipani E Maes C Carmeliet G et al Regulation of osteogenesis-angiogenesis coupling by HIFs and VEGF. J Bone Miner Res 2009; 24: 1347–1353. https://doi.org/10.1359/jbmr.09060210.1359/jbmr.090602 ↗
12. Stegen S van Gastel N Carmeliet G. Bringing new life to damaged bone: the importance of angiogenesis in bone repair and regeneration. Bone 2015; 70: 19–27. https://doi.org/10.1016/j.bone.2014.09.01710.1016/j.bone.2014.09.017 ↗
13. Stefanini MO Wu FT Mac Gabhann F et al A compartment model of VEGF distribution in blood healthy and diseased tissues. BMC Syst Biol 2008; 2: 77. https://doi.org/10.1186/1752-0509-2-7710.1186/1752-0509-2-77 ↗
14. Yamakawa S Hayashida K. Advances in surgical applications of growth factors for wound healing. Burns Trauma 2019; 7: 10. https://doi.org/10.1186/s41038-019-0148-110.1186/s41038-019-0148-1 ↗
15. Simons M Ware JA. Therapeutic angiogenesis in cardiovascular disease. Nat Rev Drug Discov 2003; 2: 863–871. https://doi.org/10.1038/nrd122610.1038/nrd1226 ↗
16. Ozawa CR Banfi A Glazer NL et al Microenvironmental VEGF concentration not total dose determines a threshold between normal and aberrant angiogenesis. J Clin Investig 2004; 113: 516–527. https://doi.org/10.1172/jci1842010.1172/JCI18420 ↗
17. Grosso A Lunger A Burger MG et al VEGF dose controls the coupling of angiogenesis and osteogenesis in engineered bone. NPJ Regen Med 2023; 8: 15. https://doi.org/10.1038/s41536-023-00288-110.1038/s41536-023-00288-1 ↗
18. 10.1126/science.114724110.1126/science.1147241 ↗
19. 10.1039/C5MH00089K10.1039/C5MH00089K ↗
20. Bettinger CJ Bruggeman JP Misra A et al Biocompatibility of biodegradable semiconducting melanin films for nerve tissue engineering. Biomaterials 2009; 30: 3050–3057. https://doi.org/10.1016/j.biomaterials.2009.02.01810.1016/j.biomaterials.2009.02.018 ↗
21. Ku SH Ryu J Hong SK et al General functionalization route for cell adhesion on non-wetting surfaces. Biomaterials 2010; 31: 2535–2541. https://doi.org/10.1016/j.biomaterials.2009.12.02010.1016/j.biomaterials.2009.12.020 ↗
22. Ren X Zhao M Lash B et al Growth factor engineering strategies for regenerative medicine applications. Front Bioeng Biotechnol 2019; 7: 469. https://doi.org/10.3389/fbioe.2019.0046910.3389/fbioe.2019.00469 ↗
23. Ozcan C Hasirci N. Plasma modification of PMMA films: surface free energy and cell-attachment studies. J Biomater Sci Polym Ed 2007; 18: 759–773. https://doi.org/10.1163/15685620778103412410.1163/156856207781034124 ↗
24. Ryu J Ku SH Lee H et al Mussel-inspired polydopamine coating as a universal route to hydroxyapatite crystallization. Adv Funct Mater 2010; 20: 2132–2139. https://doi.org/10.1002/adfm.20090234710.1002/adfm.200902347 ↗
25. Godoy-Gallardo M Portolés-Gil N López-Periago AM et al Immobilization of BMP-2 and VEGF within multilayered polydopamine-coated scaffolds and the resulting osteogenic and angiogenic synergy of Co-cultured human mesenchymal stem cells and human endothelial progenitor cells. Int J Mol Sci 2020; 21(17): 6418. https://doi.org/10.3390/ijms2117641810.3390/ijms21176418 ↗
26. Wang Z Chen L Wang Y et al Improved cell adhesion and osteogenesis of op-HA/PLGA composite by poly(dopamine)-assisted immobilization of collagen mimetic peptide and osteogenic growth peptide. ACS Appl Mater Interfaces 2016; 8: 26559–26569. https://doi.org/10.1021/acsami.6b0873310.1021/acsami.6b08733 ↗
27. Ma L Cheng S Ji X et al Immobilizing magnesium ions on 3D printed porous tantalum scaffolds with polydopamine for improved vascularization and osteogenesis. Mater Sci Eng C Mater Biol Appl 2020; 117: 111303. https://doi.org/10.1016/j.msec.2020.11130310.1016/j.msec.2020.111303 ↗
28. Liu G Ma M Yang H et al Chitosan/polydopamine/octacalcium phosphate composite microcarrier simulates natural bone components to induce osteogenic differentiation of stem cells. Biomater Adv 2023; 154: 213642. https://doi.org/10.1016/j.bioadv.2023.21364210.1016/j.bioadv.2023.213642 ↗
29. Tan L Tang W Liu T et al Biocompatible hollow polydopamine nanoparticles loaded ionic liquid enhanced tumor microwave thermal ablation in vivo. ACS Appl Mater Interfaces 2016; 8: 11237–11245. https://doi.org/10.1021/acsami.5b1232910.1021/acsami.5b12329 ↗
30. Pripatnanont P Chankum C Meesane J et al Physical and biological performances of a semi-resorbable barrier membrane based on silk fibroin-glycerol-fish collagen material for guided bone regeneration. J Biomater Appl 2021; 36: 930–942. https://doi.org/10.1177/0885328221102578110.1177/08853282211025781 ↗
31. Bucher T Clodt JI Grabowski A et al Colour-value based method for polydopamine coating-stability characterization on polyethersulfone membranes. Membranes 2017; 7: 20171216. https://doi.org/10.3390/membranes704007010.3390/membranes7040070 ↗
32. 10.3390/polym1412245110.3390/polym14122451 ↗
33. Hwang TI Kim JI Joshi MK et al Simultaneous regeneration of calcium lactate and cellulose into PCL nanofiber for biomedical application. Carbohydr Polym 2019; 212: 21–29. https://doi.org/10.1016/j.carbpol.2019.01.08510.1016/j.carbpol.2019.01.085 ↗
34. Lino AB McCarthy AD Fernández JM. Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: in vitro and in vivo studies. Ann Biomed Eng 2019; 47: 902–912. https://doi.org/10.1007/s10439-018-02183-z10.1007/s10439-018-02183-z ↗
35. Pripatnanont P Praserttham P Suttapreyasri S et al Bone regeneration potential of biphasic nanocalcium phosphate with high hydroxyapatite/tricalcium phosphate ratios in rabbit calvarial defects. Int J Oral Maxillofac Implants 2016; 31: 294–303. https://doi.org/10.11607/jomi.453110.11607/jomi.4531 ↗
36. Aguirre A Planell JA Engel E. Dynamics of bone marrow-derived endothelial progenitor cell/mesenchymal stem cell interaction in co-culture and its implications in angiogenesis. Biochem Biophys Res Commun 2010; 400: 284–291. https://doi.org/10.1016/j.bbrc.2010.08.07310.1016/j.bbrc.2010.08.073 ↗
37. 10.2306/scienceasia1513-1874.2019.45.15910.2306/scienceasia1513-1874.2019.45.159 ↗
38. Hong S Na YS Choi S et al Non-covalent self-assembly and covalent polymerization co-contribute to polydopamine Formation. Adv Funct Mater 2012; 22: 4711–4717. https://doi.org/10.1002/adfm.20120115610.1002/adfm.201201156 ↗
39. Kang SM Hwang NS Yeom J et al One-step multipurpose surface functionalization by adhesive catecholamine. Adv Funct Mater 2012; 22: 2949–2955. https://doi.org/10.1002/adfm.20120017710.1002/adfm.201200177 ↗
40. 10.1021/acsami.7b1986510.1021/acsami.7b19865 ↗
41. Gkogkos G Panariello L Grammenou E et al Synthesis of PEG-PPG-PEG templated polydopamine nanoparticles under intensified conditions: Kinetics investigation continuous process design and demonstration for photothermal application. Chem Eng J 2023; 467: 143350. https://doi.org/10.1016/j.cej.2023.14335010.1016/j.cej.2023.143350 ↗
42. Street J Winter D Wang JH et al Is human fracture hematoma inherently angiogenic? Clin Orthop Relat Res 2000; 378: 224–237. https://doi.org/10.1097/00003086-200009000-0003310.1097/00003086-200009000-00033 ↗
43. 10.1182/blood.V87.8.3336.bloodjournal878333610.1182/blood.V87.8.3336.bloodjournal8783336 ↗
44. Street J Bao M deGuzman L et al Vascular endothelial growth factor stimulates bone repair by promoting angiogenesis and bone turnover. Proc Natl Acad Sci USA 2002; 99: 9656–9661. https://doi.org/10.1073/pnas.15232409910.1073/pnas.152324099 ↗
45. Tarkka T Sipola A Jämsä T et al Adenoviral VEGF-A gene transfer induces angiogenesis and promotes bone formation in healing osseous tissues. J Gene Med 2003; 5: 560–566. https://doi.org/10.1002/jgm.39210.1002/jgm.392 ↗
46. Kempen DH Lu L Heijink A et al Effect of local sequential VEGF and BMP-2 delivery on ectopic and orthotopic bone regeneration. Biomaterials 2009; 30: 2816–2825. https://doi.org/10.1016/j.biomaterials.2009.01.03110.1016/j.biomaterials.2009.01.031 ↗
47. Pufe T Wildemann B Petersen W et al Quantitative measurement of the splice variants 120 and 164 of the angiogenic peptide vascular endothelial growth factor in the time flow of fracture healing: a study in the rat. Cell Tissue Res 2002; 309: 387–392. https://doi.org/10.1007/s00441-002-0605-010.1007/s00441-002-0605-0 ↗
48. Uchida S Sakai A Kudo H et al Vascular endothelial growth factor is expressed along with its receptors during the healing process of bone and bone marrow after drill-hole injury in rats. Bone 2003; 32: 491–501. https://doi.org/10.1016/s8756-3282(03)00053-x10.1016/S8756-3282(03)00053-X ↗