Selectivity of Antibody-Targeted Anthracycline Antibiotics on T Lymphocytes
Source abstract
Abstract
Targeted polymeric prodrugs based on N-(2-hydroxypropyl) methacrylamide were tested on human peripheral blood lymphocytes or mouse splenocytes triggered in vitro to proliferation by T cell specific (Con A) or B cell specific (S. aureus Cowan 1) mitogens. Only a selective inhibition of 3 H- thymidine incorporation by T lymphocytes was observed after in vitro incuba tion with prodrugs prepared by covalent attachment of daunomycin or adriamycin and antibody (anti-CD3; anti Thy 1.2) to biodegradable oligopeptide side chains of soluble synthetic copolymer HPMA. The in vitro results were confirmed in vivo by inhibition of antibody response to thymus dependent (ARS-BGG) or thymus independent (TNP-E. coli) antigens in normal Balb/c mice and in athymic nu/nu Balb/c mice. After administration of biocompatible, antibody targeted prodrugs only the antibody response against ARS-BGG was considerably reduced.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Monoclonal and Polyclonal Antibodies Research · Glycosylation and Glycoproteins Research · HER2/EGFR in Cancer Research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Institute of Microbiology Czechoslovak Academy of Sciences 142 20 Prague 4, Czechoslovakia
Institute of Macromolecular Chemistry Czechoslovak Academy of Sciences 162 06 Prague 6, Czechoslovakia
References (38)
Show source-provided references
1. Chabner, B.A. · Semin. Oncol. · 1977 · 4 · 165
2. 10.1016/0014-2964(77)90114-110.1016/0014-2964(77)90114-1 ↗
3. 10.1038/256495a010.1038/256495a0 ↗
4. 10.1111/j.1600-065X.1982.tb00393.x10.1111/j.1600-065X.1982.tb00393.x ↗
5. Goldberg, E. P. · 1983
6. Kopecek, J. · 1984
7. 10.1016/0006-2944(85)90135-810.1016/0006-2944(85)90135-8 ↗
8. 10.1177/08839115880030010310.1177/088391158800300103 ↗
9. Kopecek, J. 1982. In IUPAC Macromolecules. H. Benoit and P. Rempp, eds. Oxford: Pergamon Press, p. 305.
10. Kopecek, J., P. Rejmanova, J. Strohalm, K. Ulbrich, B. Rihova, V. Chytry, R. Duncan and J.B. Lloyd. 1985. Pat. Appl., PV 97-85.
11. Kopecek, J. and R. Duncan. 1987. In Polymers in Controlled Drug Delivery . L. Illum and S. S. Davis, eds. Bristol: Wright , p. 152.
12. 10.1016/0168-3659(85)90052-510.1016/0168-3659(85)90052-5 ↗
13. 10.1016/S0378-4347(00)80839-710.1016/S0378-4347(00)80839-7 ↗
14. 10.1016/0090-1229(88)90010-410.1016/0090-1229(88)90010-4 ↗
15. 10.1016/0168-3659(89)90030-810.1016/0168-3659(89)90030-8 ↗
16. 10.1177/08839115860010010310.1177/088391158600100103 ↗
17. 10.1016/0142-9612(84)90048-610.1016/0142-9612(84)90048-6 ↗
18. Flanagan, P.A. · Biochim. Biophys. Acta · 1990
19. Flanagan, P.A. · Brit. J. Cancer · 1989 · 60 · 485
20. 10.1002/macp.1990.02191041210.1002/macp.1990.021910412 ↗
21. 10.1038/bjc.1987.3310.1038/bjc.1987.33 ↗
22. 10.1117/12.96018710.1117/12.960187 ↗
23. 10.1038/bjc.1988.3110.1038/bjc.1988.31 ↗
24. 10.1002/apmc.1978.05070011010.1002/apmc.1978.050700110 ↗
25. 10.1002/macp.1977.02178080310.1002/macp.1977.021780803 ↗
26. 10.1016/0142-9612(89)90075-610.1016/0142-9612(89)90075-6 ↗
27. 10.1002/macp.1977.02178080410.1002/macp.1977.021780804 ↗
28. 10.1093/infdis/89.2.10910.1093/infdis/89.2.109 ↗
29. Tlaskalová, H. · Folia Biol. · 1970 · 16 · 102
30. 10.1007/BF0288408510.1007/BF02884085 ↗
31. 10.1111/j.1600-065X.1987.tb01174.x10.1111/j.1600-065X.1987.tb01174.x ↗
32. 10.1016/0168-3659(87)90085-X10.1016/0168-3659(87)90085-X ↗
33. Klein, J. · 1982
34. 10.1016/S0378-4347(00)80856-710.1016/S0378-4347(00)80856-7 ↗
35. Holub, M. · 1990
36. 10.1016/0168-3659(89)90020-510.1016/0168-3659(89)90020-5 ↗
37. Kopecek, J. · Proc. SPIE · 1990 · 998
38. Ríhová, B. · Immunopharmacology · 1990