DataVXAdvanced BiologyPaper
journal article·2023-08Open access

Serial Block Face‐Scanning Electron Microscopy as a Burgeoning Technology

Andrea G. Marshall, Kit Neikirk, Dominique C. Stephens, Larry Vang, Zer Vue, Heather K. Beasley, Amber Crabtree, Estevão Scudese, Edgar Garza Lopez, Bryanna Shao, Evan Krystofiak, Sharifa Rutledge, Jaimaine Davis, Sandra A. Murray, Steven M. Damo, Prasanna Katti, Antentor Hinton

Advanced Biology· Volume 7· Issue 8
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Source abstract

Abstract

Abstract Serial block face scanning electron microscopy (SBF‐SEM), also referred to as serial block‐face electron microscopy, is an advanced ultrastructural imaging technique that enables three‐dimensional visualization that provides largerx‐ and y‐axis ranges than other volumetric EM techniques. While SEM is first introduced in the 1930s, SBF‐SEM is developed as a novel method to resolve the 3D architecture of neuronal networks across large volumes with nanometer resolution by Denk and Horstmann in 2004. Here, the authors provide an accessible overview of the advantages and challenges associated with SBF‐SEM. Beyond this, the applications of SBF‐SEM in biochemical domains as well as potential future clinical applications are briefly reviewed. Finally, the alternative forms of artificial intelligence‐based segmentation which may contribute to devising a feasible workflow involving SBF‐SEM, are also considered.
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Research context

Nanomaterials

OpenAlex topics: Advanced Electron Microscopy Techniques and Applications · Electron and X-Ray Spectroscopy Techniques · Anodic Oxide Films and Nanostructures

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Molecular Physiology and Biophysics Vanderbilt University Nashville TN 37232 USA

Laboratory of Biosciences of Human Motricity (LABIMH) of the Federal University of State of Rio de Janeiro (UNIRIO) Rio de Janeiro Brazil

Sport Sciences and Exercise Laboratory (LaCEE) Catholic University of Petrópolis (UCP) Catholic 25685‐100 Brazil

Department of Internal Medicine University of Iowa Iowa City IA 52242 USA

Department of Cell and Developmental Biology Vanderbilt University Nashville TN 37232 USA

Department of Chemistry University of Alabama in Huntsville Huntsville AL 35899 USA

Department of Biochemistry, Cancer Biology, Neuroscience, Pharmacology Meharry Medical College Nashville TN 37232 USA

Department of Cell Biology University of Pittsburgh Pittsburgh PA 15261 USA

Department of Life and Physical Sciences Fisk University Nashville TN 37208 USA

Center for Structural Biology Vanderbilt University Nashville TN 37232 USA

National Heart, Lung and Blood Institute National Institutes of Health 9000 Rockville Pike Bethesda MD 20892 USA

Funding

National Institutes of Health · Chan Zuckerberg Initiative · Silicon Valley Community Foundation · National Science Foundation · Burroughs Wellcome Fund

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