DataVXAdvanced BiologyPaper
journal article·2021-06Open access

Electrochemical Impedance Spectroscopy as a Tool for Monitoring Cell Differentiation from Floor Plate Progenitors to Midbrain Neurons in Real Time

Aya Elghajiji, Xin Wang, Stephen D. Weston, Guenther Zeck, Bastian Hengerer, David Tosh, Paulo R. F. Rocha

Advanced Biology· Volume 5· Issue 6
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Source abstract

Abstract

Abstract Here shows that electrical impedance spectroscopy can be used as a non‐invasive and real time tool to probe cell adhesion and differentiation from midbrain floor plate progenitors into midbrain neurons on Au electrodes coated with human laminin. The electrical data and equivalent circuit modeling are consistent with standard microscopy analysis and reveal that within the first 6 hours progenitor cells sediment and attach to the electrode within 40 hours. Between 40 and 120 hours, midbrain progenitor cells differentiate into midbrain neurons, followed by an electrochemically stable maturation phase. The ability to sense and characterize non‐invasively and in real time cell differentiation opens up unprecedented avenues for implantable therapies and differentiation strategies.
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Research context

Biomaterials

OpenAlex topics: Neuroscience and Neural Engineering · Microfluidic and Bio-sensing Technologies · 3D Printing in Biomedical Research

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Affiliations

Centre for Biosensors Bioelectronics and Biodevices (C3Bio) Department of Electronic and Electrical Engineering University of Bath Claverton Down Bath BA2 7AY United Kingdom

Centre for Regenerative Medicine Department of Biology and Biochemistry University of Bath Claverton Down Bath BA2 7AY United Kingdom

Biomedical Electronics and Systems Institute of Electrodynamics Microwave and Circuit Engineering Vienna University of Technology Wien A‐1040 Austria

CNS Diseases Research Boehringer Ingelheim Pharma GmbH & Co. KG Biberach 88397 Germany

Centre for Functional Ecology (CFE) Department of Life Sciences University of Coimbra Coimbra 3000–456 Portugal

Funding

Schlumberger Foundation · European Research Council

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