Raman Microspectroscopy Imaging Analysis of Extracellular Vesicles Biogenesis by Filamentous Fungus Penicilium chrysogenum
Source abstract
Abstract
Abstract The mechanism of production of extracellular vesicles (EVs) and their molecular contents are of great interest due to their diverse roles in biological systems and are far from being completely understood. Even though cellular cargo releases mediated by EVs have been demonstrated in several cases, their role in secondary metabolite production and release remains elusive. In this study, this aspect is investigated in detail using Raman microspectroscopic imaging. Considerable evidence is provided to suggest that the release of antibiotic penicillin by the filamentous fungus Penicillium chrysogenum involves EVs. Further, the study also reveals morphological modifications of the fungal body during biogenesis, changes in cell composition at the locus of biogenesis, and major molecular contents of the released EVs. The results suggest a possible general role of EVs in the release of antibiotics from the producing organisms.
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Research context
OpenAlex topics: Extracellular vesicles in disease · Bacterial Infections and Vaccines · Antimicrobial Peptides and Activities
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Research Organization for Nano and Life Innovations Waseda University 513 Wasedatsurumaki‐cho Shinjuku‐ku Tokyo 162‐0041 Japan
Department of Advanced Science Engineering Waseda University Japan 3‐4‐1 Okubo Shinjuku‐ku Tokyo 169‐8555 Japan
Computational Bio Big‐Data Open Innovation Laboratory AIST‐Waseda University Japan 3‐4‐1 Okubo Shinjuku‐ku Tokyo 169‐8555 Japan
Department of Life Science and Medical Bioscience Waseda University 2‐2 Wakamatsu‐cho Shinjuku‐ku Tokyo 162‐8480 Japan
Institute for Advanced Research of Biosystem Dynamics Waseda Research Institute for Science and Engineering Graduate School of Advanced Science and Engineering Waseda University 3‐4‐1 Okubo Shinjuku‐ku Tokyo 169‐8555 Japan
References (66)
Show source-provided references
1. 10.3389/fmicb.2018.0150210.3389/fmicb.2018.01502 ↗
2. 10.7150/thno.2057610.7150/thno.20576 ↗
3. 10.1186/s12943-019-0965-710.1186/s12943-019-0965-7 ↗
4. 10.1146/annurev-cellbio-101512-12232610.1146/annurev-cellbio-101512-122326 ↗
5. 10.1084/jem.183.3.116110.1084/jem.183.3.1161 ↗
6. 10.1038/8543810.1038/85438 ↗
7. 10.1038/nature1537610.1038/nature15376 ↗
8. 10.1016/S0021-9258(18)48095-710.1016/S0021-9258(18)48095-7 ↗
9. 10.1038/nrmicro348010.1038/nrmicro3480 ↗
10. 10.1371/journal.pone.005466110.1371/journal.pone.0054661 ↗
11. 10.3389/fmicb.2017.0178310.3389/fmicb.2017.01783 ↗
12. Murphy D. E. · Exp. Mol. Med. · 2019 · 59 · 2037
13. 10.1146/annurev-pharmtox-010814-12463010.1146/annurev-pharmtox-010814-124630 ↗
14. 10.1073/pnas.100884310710.1073/pnas.1008843107 ↗
15. 10.1111/j.1749-6632.1966.tb52382.x10.1111/j.1749-6632.1966.tb52382.x ↗
16. 10.1038/nrmicro352510.1038/nrmicro3525 ↗
17. 10.1007/s12275-012-1589-410.1007/s12275-012-1589-4 ↗
18. 10.1038/ncomms1122010.1038/ncomms11220 ↗
19. 10.1038/s41467-017-00492-w10.1038/s41467-017-00492-w ↗
20. 10.1038/s41579-018-0112-210.1038/s41579-018-0112-2 ↗
21. 10.1128/mBio.00207-1610.1128/mBio.00207-16 ↗
22. 10.1128/EC.00318-0610.1128/EC.00318-06 ↗
23. 10.1016/j.mib.2013.04.00210.1016/j.mib.2013.04.002 ↗
24. 10.1186/s12866-014-0324-110.1186/s12866-014-0324-1 ↗
25. 10.1128/IAI.05769-1110.1128/IAI.05769-11 ↗
26. 10.1271/bbb.10075410.1271/bbb.100754 ↗
27. 10.1128/mBio.00940-1610.1128/mBio.00940-16 ↗
28. 10.1128/JB.01463-1310.1128/JB.01463-13 ↗
29. 10.3402/jev.v5.3257010.3402/jev.v5.32570 ↗
30. 10.1194/jlr.R08464010.1194/jlr.R084640 ↗
31. 10.1016/j.bbalip.2013.04.01110.1016/j.bbalip.2013.04.011 ↗
32. 10.1038/s41556-018-0040-410.1038/s41556-018-0040-4 ↗
33. 10.1016/j.copbio.2011.03.01010.1016/j.copbio.2011.03.010 ↗
34. 10.1038/nature1295910.1038/nature12959 ↗
35. 10.1039/C5NP00111K10.1039/C5NP00111K ↗
36. 10.1038/s42003-020-1100-410.1038/s42003-020-1100-4 ↗
37. 10.1021/acs.analchem.1c0294210.1021/acs.analchem.1c02942 ↗
38. 10.1021/acs.jnatprod.0c0021410.1021/acs.jnatprod.0c00214 ↗
39. 10.1002/jrs.249310.1002/jrs.2493 ↗
40. 10.1006/abio.1994.135110.1006/abio.1994.1351 ↗
41. 10.1177/0284185195036S3990310.1177/0284185195036S39903 ↗
42. 10.1038/srep2778910.1038/srep27789 ↗
43. 10.1128/AEM.01525-1210.1128/AEM.01525-12 ↗
44. 10.3390/jof602006810.3390/jof6020068 ↗
45. 10.1007/s00294-014-0454-x10.1007/s00294-014-0454-x ↗
46. 10.1111/j.1365-2672.2006.02982.x10.1111/j.1365-2672.2006.02982.x ↗
47. 10.33073/pjm-2014-05410.33073/pjm-2014-054 ↗
48. 10.1128/AAC.31.11.167510.1128/AAC.31.11.1675 ↗
49. 10.1038/nbt.149810.1038/nbt.1498 ↗
50. 10.1002/j.1460-2075.1991.tb07971.x10.1002/j.1460-2075.1991.tb07971.x ↗
51. 10.1371/journal.pone.003549010.1371/journal.pone.0035490 ↗
52. 10.1016/j.fgb.2004.10.01010.1016/j.fgb.2004.10.010 ↗
53. 10.5604/17331331.121561610.5604/17331331.1215616 ↗
54. 10.1128/EC.00318-0610.1128/EC.00318-06 ↗
55. 10.1111/j.1751-7915.2009.00123.x10.1111/j.1751-7915.2009.00123.x ↗
56. 10.1117/1.JBO.19.1.01101610.1117/1.JBO.19.1.011016 ↗
57. 10.1038/4456510.1038/44565 ↗
58. 10.1002/cem.118004010310.1002/cem.1180040103 ↗
59. 10.1038/nprot.2015.00810.1038/nprot.2015.008 ↗
60. 10.1021/acs.analchem.5b0407510.1021/acs.analchem.5b04075 ↗
61. 10.1007/978-1-62703-245-2_1610.1007/978-1-62703-245-2_16 ↗
62. 10.1002/jrs.139710.1002/jrs.1397 ↗
63. 10.1007/s00216-010-3566-210.1007/s00216-010-3566-2 ↗
64. 10.1186/1746-4811-10-1410.1186/1746-4811-10-14 ↗
65. 10.1073/pnas.110752410810.1073/pnas.1107524108 ↗
66. 10.1002/jbio.20120004210.1002/jbio.201200042 ↗