The PD‐1 Interactome (Adv. Biology 9/2021)

Programmed Death-1 In article 2100758, Boussiotis, Patsoukis, Wang, and Bardhan show that Venus-based biomolecular fluorescence complementation detects Programmed Death-1 (PD-1) dimerization by confocal microscopy. HEK-293 cells are transfected with PD-1-VenusYFP-N-terminus and PD-1-VenusYFP-C-terminus together with SHP-2 and either kinase active (top) or kinase inactive (bottom) Fyn. YFP signal (yellow) from VenusYFP structural complementation, indicating PD-1 dimerization at the plasma membrane, is generated only in the presence of kinase active Fyn (red: Rhodamine Phalloidin; blue: DAPI nuclear staining).