Low molecular weight PEI-grafted carboxyl-modified soybean protein as gene carriers with reduced cytotoxicity and greatly improved transfection in vitro
Source abstract
Abstract
SA-SP-PEI were synthesized via acylation reaction between carboxyl-modified soybean protein (SA-SP) and branched polyethylenimine (PEI) with molecular weight of 600, 1200, and 1800 Da, and designed as SA-SP-PEI600, SA-SP-PEI1200, and SA-SP-PEI1800, respectively. SA-SP-PEI could effectively condense plasmid DNA into nanoscale polyplexes and protect them from enzymatic digestion. MTT assay revealed that SA-SP-PEI exhibited reduced cytotoxicity on 293 T and SH-SY5Y cells. SA-SP-PEI1800/DNA complexes hold highest transfection efficiency on 293 T and SH-SY5Y cells with or without 10% serum, which was owing to its better serum stable and improved biocompatibility. Such polycationic soybean proteins have great potentials as gene carriers by further optimization.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: RNA Interference and Gene Delivery · Animal Genetics and Reproduction · Electrospun Nanofibers in Biomedical Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Engineering Research Center for Biomedical Materials, School of Life Science, Anhui Key Laboratory of Modern Biomanufacturing Anhui University, Hefei, Anhui Province, P. R. China
Funding
National Natural Science Foundation of China · Doctor Research Foundation of Anhui University of China · Academic and Technology Introduction Project of Anhui University of China · Nature Science Research Programme of the Education Office of Anhui Province
References (46)
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