Incorporation of chitosan nanoparticles into silk fibroin-based porous scaffolds: Chondrogenic differentiation of stem cells

Mitra Naeimi; Mohammad Rafienia; Mohammadhossein Fathi; Mohsen Janmaleki; Shahin Bonakdar; Mehdi Ebrahimian-Hosseinabadi

doi:10.1080/00914037.2015.1099103

journal article·2016-03-03

Incorporation of chitosan nanoparticles into silk fibroin-based porous scaffolds: Chondrogenic differentiation of stem cells

Mitra Naeimi, Mohammad Rafienia, Mohammadhossein Fathi, Mohsen Janmaleki, Shahin Bonakdar, Mehdi Ebrahimian-Hosseinabadi

International Journal of Polymeric Materials and Polymeric Biomaterials· Volume 65· Issue 4

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Abstract

A silk fibroin-chondroitin sulfate-sodium alginate (SF-CHS-SA) porous scaffold containing chitosan nanoparticles (NPs) was investigated. The proliferation of adipose-derived stem cells (ASCs) was studied by SEM, fluorescent microscopy, alcian blue staining, dimethylmethylene blue assay, and real-time polymerization chain reaction. The results showed that incorporation of NPs into the scaffold improved compressive modulus (5.6 ± 0.15 MPa). The amount of glycosaminoglycan expression of the ASCs was reached to 8.9 ± 0.3 µg/mL. The gene expressions of aggrecan, collagen II, and SOX9 of the ASCs were significantly improved. This study revealed that the prepared scaffold can be used as a substrate for cartilage tissue engineering.

Source: openalex_inverted_index · Quality: baseline

Research context

Polymers Nanomaterials Biomaterials

OpenAlex topics: Silk-based biomaterials and applications · Osteoarthritis Treatment and Mechanisms · Electrospun Nanofibers in Biomedical Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

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