Surface Modification of Nanoporous Poly(ϵ-caprolactone) Membrane with Poly(ethylene glycol) to Prevent Biofouling: Part I. Effects of Plasma Power and Treatment Time

Abstract Biofouling, a result of protein adsorption and cell adhesion on a surface, is detrimental to membrane performance. The objective of this study is to modify the polycaprolactone (PCL) membrane surface with poly(ethylene glycol) (PEG) to prevent fibroblast adhesion. To achieve this goal, oxygen plasma and PEG(400)-monoacrylate were used to graft the PEG onto the membrane surface through covalent bonding. Various plasma treatment conditions were investigated to optimize the PEG-grafting quality and to achieve minimum fibroblast adhesion. After the treatment, the water contact angle decreased significantly. Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra indicated that PEG was successfully grafted onto the PCL membrane with the appearance of the PEG characteristic peaks. X-ray photoelectron spectroscopy (XPS) revealed that different plasma powers and treatment times changed the surface composition of membranes. To evaluate the applicability of this new strategy for the prevention of biofouling, NIH 3T3 fibroblast was used as a model biofoulant. Cell adhesion and morphology studies indicate that either lower plasma power or shorter treatment time is able to improve resistance to the cell adhesion. This simple and efficient method can be applied to inhibit biofouling on the membrane surface. Keywords: biofoulingoxygen plasmapoly(ethylene glycol) (PEG)polycaprolactone (PCL) membranesurface modification We would like to thank the National Science Foundation-sponsored Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices (NSEC-CANPBD) at the Ohio State University for the financial support of this work.