Biological Activity of Extracts and Kombucha Ferments Obtained from Melliferous Plant Flowers (Tilia L. and Calluna vulgaris L.): Antioxidant, Cytotoxic, Anti-Inflammatory and Antibacterial Properties
Source abstract
Abstract
Flowers of melliferous plants, such as Tilia L. and Calluna vulgaris L., are rich in highly active polyphenols and flavonoids. The aim of this study was to evaluate the biological properties of extracts and kombucha ferments from Tilia L. and Calluna vulgaris L. flowers. The phytochemical profile was analyzed by LC-MS. Antioxidant potential was assessed using ABTS and DPPH assays, as well as by measuring intracellular ROS levels in skin cells. Cytotoxicity was determined using Alamar Blue assay in in vitro models. Anti-inflammatory activity was assessed based on IL-6, TNF-α, and COX-2 levels, and antibacterial properties were assessed against Gram-positive and Gram-negative bacteria. ABTS and DPPH assays showed the greatest antioxidant potential for Calluna vulgaris L. 7-day ferments, achieving 90.41 and 63.24% reductions in these radicals, respectively. Resazurin tests showed that in most cases, sample concentrations not exceeding 250 µg/mL did not cause a cytotoxic effect. In anti-inflammatory tests, the compound most strongly inhibited IL-6, reducing the level of this cytokine from 3.55-fold (for the positive control with LPS) to only 2.53-fold. The results demonstrated antimicrobial activity, with a zone of inhibition for S. aureus of 16 mm. Overall, the results indicate that Tilia L. and Calluna vulgaris L. flower extracts and kombucha ferments represent promising natural sources of bioactive compounds with antioxidant, antibacterial, and anti-inflammatory properties.
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Research context
OpenAlex topics: Tea Polyphenols and Effects · Tannin, Tannase and Anticancer Activities · Medicinal plant effects and applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Technology of Cosmetic and Pharmaceutical Products, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, Poland
Department of Analytical Chemistry, Medical University of Lublin, Aleje Raclawickie 1, 20-059 Lublin, Poland
Department of Neurosurgery and Paediatric Neurosurgery, Medical University of Lublin, 20-090 Lublin, Poland
References (91)
Show source-provided references
1. Hoang, H.T., Moon, J.Y., and Lee, Y.C. (2021). Natural Antioxidants from Plant Extracts in Skincare Cosmetics: Recent Applications, Challenges and Perspectives. Cosmetics, 8.10.3390/cosmetics8040106 ↗
2. An Overview of Cosmeceutically Relevant Plant Extracts and Strategies for Extraction of Plant-Based Bioactive Compounds10.1016/j.fbp.2018.09.002 ↗
3. Matos, M.S., Romero-Díez, R., Álvarez, A., Bronze, M.R., Rodríguez-Rojo, S., Mato, R.B., Cocero, M.J., and Matias, A.A. (2019). Polyphenol-Rich Extracts Obtained from Winemaking Waste Streams as Natural Ingredients with Cosmeceutical Potential. Antioxidants, 8.10.3390/antiox8090355 ↗
4. Michalak, M. (2022). Plant-Derived Antioxidants: Significance in Skin Health and the Ageing Process. Int. J. Mol. Sci., 23.10.3390/ijms23020585 ↗
5. Majchrzak, W., Motyl, I., and Śmigielski, K. (2022). Biological and Cosmetical Importance of Fermented Raw Materials: An Overview. Molecules, 27.10.20944/preprints202207.0245.v1 ↗
6. Ziemlewska, A., Nizioł-Łukaszewska, Z., Zagórska-Dziok, M., Mokrzyńska, A., Krupski, W., Wójciak, M., and Sowa, I. (2025). Anti-Aging, Anti-Inflammatory, and Cytoprotective Properties of Lactobacillus- and Kombucha-Fermented C. Pepo L. Peel and Pulp Extracts with Prototype Skin Toner Development. Molecules, 30.10.3390/molecules30204082 ↗
7. Understanding Kombucha Tea Fermentation: A Review10.1111/1750-3841.14068 ↗
8. Influence of Starter Cultures on the Antioxidant Activity of Kombucha Beverage10.1016/j.foodchem.2011.02.048 ↗
9. Mineral and Water Soluble Vitamin Content in the Kombucha Drink10.1046/j.1365-2621.2000.00342.x ↗
10. Ziemlewska, A., Zagórska-Dziok, M., Mokrzyńska, A., Nizioł-Łukaszewska, Z., Szczepanek, D., Sowa, I., and Wójciak, M. (2024). Comparison of Anti-Inflammatory and Antibacterial Properties of Raphanus Sativus L. Leaf and Root Kombucha-Fermented Extracts. Int. J. Mol. Sci., 25.10.3390/ijms25115622 ↗
11. Nizioł-Łukaszewska, Z., Ziemlewska, A., Zagórska-Dziok, M., Mokrzyńska, A., Wójciak, M., and Sowa, I. (2025). Apiaceae Bioferments Obtained by Fermentation with Kombucha as an Important Source of Active Substances for Skin Care. Molecules, 30.10.3390/molecules30050983 ↗
12. Zagórska-Dziok, M., Ziemlewska, A., Mokrzyńska, A., Nizioł-Łukaszewska, Z., Sowa, I., Szczepanek, D., and Wójciak, M. (2023). Comparative Study of Cytotoxicity and Antioxidant, Anti-Aging and Antibacterial Properties of Unfermented and Fermented Extract of Cornus mas L. Int. J. Mol. Sci., 24.10.3390/ijms241713232 ↗
13. Ziemlewska, A., Zagórska-Dziok, M., Nowak, A., Muzykiewicz-Szymańska, A., Wójciak, M., Sowa, I., Szczepanek, D., and Nizioł-Łukaszewska, Z. (2025). Enhancing the Cosmetic Potential of Aloe Vera Gel by Kombucha-Mediated Fermentation: Phytochemical Analysis and Evaluation of Antioxidant, Anti-Aging and Moisturizing Properties. Molecules, 30.10.3390/molecules30153192 ↗
14. Ražná, K., Žiarovská, J., Ivanišová, E., Urbanová, L., Harenčár, L., Kováčik, A., Kučka, M., and Hrubík, P. (2021). Flowers Characteristics of Selected Species of Lime-Tree (Tilia spp.) in Terms of MiRNA-Based Markers Activity, Mannose Expression and Biological Compounds Content. Forests, 12.10.3390/f12121748 ↗
15. Varbanova, E., Georgieva, D., and Stefanova, V. (2024). Quantification of the Total and Extractable Content of Micro- and Trace Elements in Linden Blossom and Infusions—The Impact of Urban Pollution on Health Risk for Samples from Plovdiv, Bulgaria. Environments, 11.10.3390/environments11120274 ↗
16. Polak, B., Jaglińska, K., Boćkowska, A., Świątek, Ł., Salwa, K., Boguszewska, A., Józefczyk, A., and Jóźwiak, G. (2026). Elderberry and Linden Flowers Ethanol–Water Extracts: Extraction Type Effect, Analysis and Biological Activity Determination. Molecules, 31.10.3390/molecules31050764 ↗
17. Antibacterial and Antioxidant Activity of Flavonoid, Glycoside and Alkaloid Extracts of Tilia Cordata10.53730/ijhs.v6nS3.6664 ↗
18. Intraspecific Variability in the Content of Phenolic Compounds, Essential Oil and Mucilage of Small-Leaved Lime (Tilia cordata Mill.) from Poland10.1016/j.indcrop.2015.10.002 ↗
19. Chepel, V., Lisun, V., and Skrypnik, L. (2020). Changes in the Content of Some Groups of Phenolic Compounds and Biological Activity of Extracts of Various Parts of Heather (Calluna vulgaris (L.) Hull) at Different Growth Stages. Plants, 9.10.3390/plants9080926 ↗
20. Kaunaite, V., Vilkickyte, G., and Raudone, L. (2022). Phytochemical Diversity and Antioxidant Potential of Wild Heather (Calluna vulgaris L.) Aboveground Parts. Plants, 11.10.3390/plants11172207 ↗
21. Bioassay-Guided Isolation of Kaempferol-3-O-β-d-Galactoside with Anti-Inflammatory and Antinociceptive Activity from the Aerial Part of Calluna vulgaris L10.1016/j.jep.2007.06.017 ↗
22. Supercritical Fluid Extraction of Heather (Calluna vulgaris) and Evaluation of Anti-Hepatitis C Virus Activity of the Extracts10.1016/j.virusres.2014.12.022 ↗
23. Phytochemistry of Heather (Calluna vulgaris (L.) Hull) and Its Altitudinal Alteration10.1007/s11101-009-9153-5 ↗
24. Cucu, A.A., Baci, G.M., Cucu, A.B., Dezsi, Ş., Lujerdean, C., Hegeduş, I.C., Bobiş, O., Moise, A.R., and Dezmirean, D.S. (2022). Calluna Vulgaris as a Valuable Source of Bioactive Compounds: Exploring Its Phytochemical Profile, Biological Activities and Apitherapeutic Potential. Plants, 11.10.3390/plants11151993 ↗
25. Wang, B., Rutherfurd-Markwick, K., Zhang, X.X., and Mutukumira, A.N. (2022). Kombucha: Production and Microbiological Research. Foods, 11.10.3390/foods11213456 ↗
26. Characteristics and Biochemical Composition of Kombucha-Fermented Tea10.26444/monz/118887 ↗
27. Kombucha: A Review of Substrates, Regulations, Composition, and Biological Properties10.1111/1750-3841.16029 ↗
28. Sawicki, J., Wójciak, W., Zuk, M., Dresler, S., Sowa, I., Skalska-Kamí Nska, A., and Wójciak, M. (2026). Impact of Preservation Techniques on Polyphenols in Aronia melanocarpa Pomace and Their Recovery by Optimized Accelerated Solvent Extraction. Appl. Sci., 16.10.3390/app16094116 ↗
29. Factors Influencing the Antioxidant Activity Determined by the ABTS. + Radical Cation Assay10.3109/10715769709097799 ↗
30. Use of a Free Radical Method to Evaluate Antioxidant Activity10.1016/S0023-6438(95)80008-5 ↗
31. A New Fluorometric Assay for Cytotoxicity Measurements in Vitro
32. Microtitre Plate-Based Antibacterial Assay Incorporating Resazurin as an Indicator of Cell Growth, and Its Application in the in Vitro Antibacterial Screening of Phytochemicals10.1016/j.ymeth.2007.01.006 ↗
33. Kowalska-Krochmal, B., and Dudek-Wicher, R. (2021). The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical Relevance. Pathogens, 10.10.3390/pathogens10020165 ↗
34. Wołosiak, R., Drużyńska, B., Derewiaka, D., Piecyk, M., Majewska, E., Ciecierska, M., Worobiej, E., and Pakosz, P. (2022). Verification of the Conditions for Determination of Antioxidant Activity by ABTS and DPPH Assays—A Practical Approach. Molecules, 27.10.3390/molecules27010050 ↗
35. de Haan, L.R., Reiniers, M.J., Reeskamp, L.F., Belkouz, A., Ao, L., Cheng, S., Ding, B., van Golen, R.F., and Heger, M. (2022). Experimental Conditions That Influence the Utility of 2′7′-Dichlorodihydrofluorescein Diacetate (DCFH2-DA) as a Fluorogenic Biosensor for Mitochondrial Redox Status. Antioxidants, 11.10.3390/antiox11081424 ↗
36. Multiple Applications of Alamar Blue as an Indicator of Metabolic Function and Cellular Health in Cell Viability Bioassays10.3390/s120912347 ↗
37. Investigation of the Alamar Blue (Resazurin) Fluorescent Dye for the Assessment of Mammalian Cell Cytotoxicity10.1046/j.1432-1327.2000.01606.x ↗
38. Alfaro, R.A., and Davis, D.D. (2007). Diclofenac. xPharm Compr. Pharmacol. Ref., 1–7.10.1016/B978-008055232-3.61588-0 ↗
39. Anantachoke, N., Duangrat, R., Sutthiphatkul, T., Ochaikul, D., and Mangmool, S. (2023). Kombucha Beverages Produced from Fruits, Vegetables, and Plants: A Review on Their Pharmacological Activities and Health Benefits. Foods, 12.10.3390/foods12091818 ↗
40. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay10.1016/S0891-5849(98)00315-3 ↗
41. DPPH Antioxidant Assay Revisited10.1016/j.foodchem.2008.08.008 ↗
42. Nguyen, V., Taine, E.G., Meng, D., Cui, T., and Tan, W. (2024). Chlorogenic Acid: A Systematic Review on the Biological Functions, Mechanistic Actions, and Therapeutic Potentials. Nutrients, 16.10.3390/nu16070924 ↗
43. Bee Bread from Anatolia: Its Chemical Composition, Phenolic and Aromatic Profiles, and Antioxidant Properties10.1080/00218839.2024.2352319 ↗
44. Effect of Fermentation on the Antioxidant Activity in Plant-Based Foods10.1016/j.foodchem.2014.03.112 ↗
45. Jakubczyk, K., Kałduńska, J., Kochman, J., and Janda, K. (2020). Chemical Profile and Antioxidant Activity of the Kombucha Beverage Derived from White, Green, Black and Red Tea. Antioxidants, 9.10.3390/antiox9050447 ↗
46. Pal Singh, B., Kelebek, H., Prangdimurti, E., Yıkmış, S., Althawab, S.A., Aljobair, M.O., Ahmed, M., Alkan, G., Türkol, M., and Karrar, E. (2026). Evaluation of the Antidiabetic Potential and Bioaccessibility of Propolis-Enriched Aronia Kombucha: An in Vitro Study. Front. Nutr., 13.10.3389/fnut.2026.1819568 ↗
47. In Vitro Studies on the Mechanisms Involved in Chemoprevention Using Calluna Vulgaris on Vascular Endothelial Cells Exposed to UVB10.1016/j.jphotobiol.2014.04.018 ↗
48. Zeb, A. (2020). Concept, Mechanism, and Applications of Phenolic Antioxidants in Foods. J. Food Biochem., 44.10.1111/jfbc.13394 ↗
49. Chlorogenic Acids, Caffeine Content and Antioxidant Properties of Green Coffee Extracts: Influence of Green Coffee Bean Preparation10.1007/s00217-016-2643-y ↗
50. Wianowska, D., and Olszowy-Tomczyk, M. (2023). A Concise Profile of Gallic Acid—From Its Natural Sources through Biological Properties and Chemical Methods of Determination. Molecules, 28.10.3390/molecules28031186 ↗
51. Yang, F., Chen, C., Ni, D., Yang, Y., Tian, J., Li, Y., Chen, S., Ye, X., and Wang, L. (2023). Effects of Fermentation on Bioactivity and the Composition of Polyphenols Contained in Polyphenol-Rich Foods: A Review. Foods, 12.10.3390/foods12173315 ↗
52. Ziemlewska, A., Nizioł-łukaszewska, Z., Zagórska-Dziok, M., Bujak, T., Wójciak, M., and Sowa, I. (2022). Evaluation of Cosmetic and Dermatological Properties of Kombucha-Fermented Berry Leaf Extracts Considered to Be By-Products. Molecules, 27.10.3390/molecules27072345 ↗
53. Polyphenol Stability and Bioavailability in Cell Culture Medium: Challenges, Limitations and Future Directions10.1016/j.ijbiomac.2024.135232 ↗
54. Hydrogen Peroxide Produced from Selective Phenolic Acids in Cell Culture Underlies Caco-2 Changes in Cell Proliferation Parameters10.1021/acs.jafc.2c08830 ↗
55. Działo, M., Mierziak, J., Korzun, U., Preisner, M., Szopa, J., and Kulma, A. (2016). The Potential of Plant Phenolics in Prevention and Therapy of Skin Disorders. Int. J. Mol. Sci., 17.10.3390/ijms17020160 ↗
56. UHPLC-DAD-MS/MS Analysis of Extracts from Linden Flowers (Tiliae flos): Differences in the Chemical Composition between Five Tilia Species Growing in Europe10.1016/j.indcrop.2020.112691 ↗
57. The Influence of Procyanidins Isolated from Small-Leaved Lime Flowers (Tilia cordata Mill.) on Human Neutrophils10.1016/j.fitote.2018.02.018 ↗
58. THP-1 Cell Line: An in Vitro Cell Model for Immune Modulation Approach10.1016/j.intimp.2014.08.002 ↗
59. Oxidative Stress and Inflammation: What Polyphenols Can Do for Us?10.1155/2016/7432797 ↗
60. Comprehensive Review of Tilia L.: Phytochemical Profiles, Edible Value, Therapeutic Potentials, and Ecological Significance10.26599/FMH.2025.9420035 ↗
61. Starchenko, G., Hrytsyk, A., Raal, A., and Koshovyi, O. (2020). Phytochemical Profile and Pharmacological Activities of Water and Hydroethanolic Dry Extracts of Calluna vulgaris (L.) Hull. Herb. Plants, 9.10.3390/plants9060751 ↗
62. Anti-Inflammatory Effects of Chlorogenic Acid in Lipopolysaccharide-Stimulated RAW 264.7 Cells10.1007/s00011-013-0674-4 ↗
63. Chlorogenic Acid Inhibits Lipopolysaccharide-Induced Cyclooxygenase-2 Expression in RAW264.7 Cells through Suppressing NF-KappaB and JNK/AP-1 Activation10.1016/j.intimp.2009.04.011 ↗
64. Tilia Species (Linden) Exert Anti-Cancer Effects on MIA PaCa-2 Cells through the Modulation of Oxidative Stress and Inflammation10.1038/s41598-025-86457-2 ↗
65. Villanueva-Bermejo, D., Vázquez, E., Villalva, M., Santoyo, S., Fornari, T., Reglero, G., and García-Risco, M.R. (2019). Simultaneous Supercritical Fluid Extraction of Heather (Calluna vulgaris L.) and Marigold (Calendula officinalis L.) and Anti-Inflammatory Activity of the Extracts. Appl. Sci., 9.10.3390/app9112245 ↗
66. A Review on Kombucha Tea-Microbiology, Composition, Fermentation, Beneficial Effects, Toxicity, and Tea Fungus10.1111/1541-4337.12073 ↗
67. Lee, H.J., and Kim, M. (2022). Skin Barrier Function and the Microbiome. Int. J. Mol. Sci., 23.10.3390/ijms232113071 ↗
68. Künili, İ.E., Akdeniz, V., Akpınar, A., Budak, Ş.Ö., Curiel, J.A., Guzel, M., Karagözlü, C., Berkel Kasikci, M., Caruana, G.P.M., and Starowicz, M. (2025). Bioactive Compounds in Fermented Foods: A Systematic Narrative Review. Front. Nutr., 12.10.3389/fnut.2025.1625816 ↗
69. Plant-Derived Fermented Products: An Interesting Concept for Human Health10.1016/j.cdnut.2024.102162 ↗
70. Michalak, M. (2023). Plant Extracts as Skin Care and Therapeutic Agents. Int. J. Mol. Sci., 24.10.3390/ijms242015444 ↗
71. Main Benefits and Applicability of Plant Extracts in Skin Care Products10.3390/cosmetics2020048 ↗
72. The Skin Microbiome in Inflammatory Skin Diseases10.1007/s13671-020-00297-z ↗
73. Huq, M.A. (2025). Extracellular Synthesis of Bioactive Silver Nanoparticles Using Brevibacillus sp. MAHUQ-41 and Their Potential Application Against Drug-Resistant Bacterial Pathogens Listeria monocytogenes and Yersinia enterocolitica. J. Funct. Biomater., 16.10.3390/jfb16070241 ↗
74. Yaqoob, A.A., Ahmad, H., Parveen, T., Ahmad, A., Oves, M., Ismail, I.M.I., Qari, H.A., Umar, K., and Mohamad Ibrahim, M.N. (2020). Recent Advances in Metal Decorated Nanomaterials and Their Various Biological Applications: A Review. Front. Chem., 8.10.3389/fchem.2020.00341 ↗
75. In Vitro Activity of Heather [Calluna vulgaris (L.) Hull] Extracts on Selected Urinary Tract Pathogens10.17305/bjbms.2014.40 ↗
76. Tilia cordata: A Potent Inhibitor of Growth and Biofilm Formation of Bacterial Clinical Isolates
77. Bouarab-Chibane, L., Forquet, V., Lantéri, P., Clément, Y., Léonard-Akkari, L., Oulahal, N., Degraeve, P., and Bordes, C. (2019). Antibacterial Properties of Polyphenols: Characterization and QSAR (Quantitative Structure-Activity Relationship) Models. Front. Microbiol., 10.10.3389/fmicb.2019.00829 ↗
78. Synergy Research: Approaching a New Generation of Phytopharmaceuticals10.1016/j.fitote.2010.11.016 ↗
79. Antibacterial Activity and Mechanism of Action of Chlorogenic Acid10.1111/j.1750-3841.2011.02213.x ↗
80. Mita, S.R., Muhtar, N.I., Kusuma, S.A.F., Sriwidodo, S., and Hendrawan, R.P. (2025). Catechins as Antimicrobial Agents and Their Contribution to Cosmetics. Cosmetics, 12.10.3390/cosmetics12010011 ↗
81. Chen, H., Wang, W., Yu, S., Wang, H., Tian, Z., and Zhu, S. (2022). Procyanidins and Their Therapeutic Potential against Oral Diseases. Molecules, 27.10.3390/molecules27092932 ↗
82. Chen, K., Peng, C., Chi, F., Yu, C., Yang, Q., and Li, Z. (2022). Antibacterial and Antibiofilm Activities of Chlorogenic Acid Against Yersinia enterocolitica. Front. Microbiol., 13.10.3389/fmicb.2022.885092 ↗
83. Jan, R., Khan, M., Asaf, S., Asif, S., and Kim, K.M. (2022). Bioactivity and Therapeutic Potential of Kaempferol and Quercetin: New Insights for Plant and Human Health. Plants, 11.10.3390/plants11192623 ↗
84. Nguyen, T.L.A., and Bhattacharya, D. (2022). Antimicrobial Activity of Quercetin: An Approach to Its Mechanistic Principle. Molecules, 27.10.3390/molecules27082494 ↗
85. Characterization of Quercetin Binding Site on DNA Gyrase10.1016/S0006-291X(03)01006-4 ↗
86. Lobiuc, A., Pavăl, N.E., Mangalagiu, I.I., Gheorghiță, R., Teliban, G.C., Amăriucăi-Mantu, D., and Stoleru, V. (2023). Future Antimicrobials: Natural and Functionalized Phenolics. Molecules, 28.10.3390/molecules28031114 ↗
87. Ottová, M., Zvonařová, K., Otta, J., Knížková, K., Cihlářová, P., Michailidu, J., Maťátková, O., and Jarošová Kolouchová, I. (2026, May 09). Antimicrobial and Antibiofilm Activity of Polyphenol-Rich Extracts from Vitis Vinifera By-Products Against Foodborne Pathogens. Available online: https://papers.ssrn.com/sol3/papers.cfm?abstract_id=6539058.10.2139/ssrn.6539058 ↗
88. Chandran, A., Wyka, J., Klein, G.R., Stefanska, B., and Kolniak-Ostek, J. (2026). Shaping the Bioactive Properties of Kombucha Drinks by Using Raw Materials Alternative to Tea. Molecules, 31.10.3390/molecules31071170 ↗
89. Alharbi, N.A. (2026). Polyphenol Metabolites in Fermented Foods: Biotransformation, Bioavailability, and Functional Roles. Front. Nutr., 13.10.3389/fnut.2026.1767453 ↗
90. Antibiotic Resistance Mechanisms and Global Resistance Patterns of Pseudomonas aeruginosa in Microbial Keratitis10.1007/s10096-026-05484-7 ↗
91. Elfadadny, A., Ragab, R.F., AlHarbi, M., Badshah, F., Ibáñez-Arancibia, E., Farag, A., Hendawy, A.O., De los Ríos-Escalante, P.R., Aboubakr, M., and Zakai, S.A. (2024). Antimicrobial Resistance of Pseudomonas Aeruginosa: Navigating Clinical Impacts, Current Resistance Trends, and Innovations in Breaking Therapies. Front. Microbiol., 15.10.3389/fmicb.2024.1374466 ↗