journal article·2026-06-19Open access

Encapsulation via the Vibrating Nozzle Technology as a Strategy for the Valorization of Pomegranate Peel Polyphenols

Danijela Šeremet, Predrag Petrović, David Plahutar, Mirna Perkušić, Tamara Matić, Aleksandra Sander, Marko Obranović, Aleksandra Vojvodić Cebin, Božidar Šantek, Draženka Komes

Applied Sciences· Volume 16· Issue 12

Abstract

The aim of the present study was to investigate the bioactive composition of pomegranate peel and to produce a phenolic-rich extract for encapsulation using vibrating nozzle technology. Conventional heat- and advanced microwave-assisted extraction of phenolic compounds were investigated, and the extract with the highest phenolic content was used to perform encapsulation with alginate-based delivery solutions containing fava bean proteins, mucin, carboxymethyl cellulose, nutriose, and collagen hydrolysates. The formulated beads were characterized for their physico-chemical (morphology, size distribution parameters, and FT-IR spectra) and bioactive (encapsulation efficiency and simulated gastrointestinal digestion) properties. The highest yields of punicalin, punicalagin, and ellagic acid (9.40, 44.51, and 3.95 mg g−1 DM, respectively) were achieved by heat-assisted extraction at 80 °C. The addition of fava bean proteins and carboxymethyl cellulose to the alginate-based delivery solutions resulted in the highest encapsulation efficiency of total phenols (83.99 and 83.78%, respectively). However, the beads formulated with fava bean proteins were irregularly shaped, while those with carboxymethyl cellulose were predominantly spherical. All beads showed limited phenolic release under simulated gastric conditions, followed by enhanced release in the intestinal phase. Overall, the obtained results indicate that encapsulation efficiency was governed by the combined effects of rheological parameters, bead morphology, and molecular interactions, highlighting the importance of a multi-parameter design approach in the development of effective delivery systems.
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Research context

OpenAlex topics: Pomegranate: compositions and health benefits · Microencapsulation and Drying Processes · Phytochemicals and Antioxidant Activities

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia

Innovation Centre, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia

Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia

Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia

Faculty of Chemical Engineering and Technology, University of Zagreb, Trg Marka Marulića 19, 10000 Zagreb, Croatia

Funding

European Union

References (49)

Show source-provided references
1. Rațu, R.N., Veleșcu, I.D., Stoica, F., Usturoi, A., Arsenoaia, V.N., Crivei, I.C., Postolache, A.N., Lipșa, F.D., Filipov, F., and Florea, A.M. (2023). Application of Agri-Food By-Products in the Food Industry. Agriculture, 13.10.3390/agriculture13081559 ↗
2. Valorization of Selected Fruit and Vegetable Wastes as Bioactive Compounds: Opportunities and Challenges10.1080/10643389.2019.1694819 ↗
3. García, P., Fredes, C., Cea, I., Lozano-Sánchez, J., Leyva-Jiménez, F.J., Robert, P., Vergara, C., and Jimenez, P. (2021). Recovery of Bioactive Compounds from Pomegranate (Punica granatum L.) Peel Using Pressurized Liquid Extraction. Foods, 10.10.3390/foods10020203 ↗
4. Pomegranate Peel Phytochemistry, Pharmacological Properties, Methods of Extraction, and its Application: A Comprehensive Review10.1021/acsomega.3c02586 ↗
5. Bioactive Compounds from Pomegranate Peels—Biological Properties, Structure–Function Relationships, Health Benefits and Food Applications—A Comprehensive review10.1016/j.jff.2024.106132 ↗
6. Encapsulation in the Food Industry: A Brief Historical Overview to Recent Developments
7. Micro and Nano Encapsulation, Retention and Controlled Release of Flavor and Aroma Compounds: A Critical Review10.1016/j.tifs.2019.02.030 ↗
8. Co-encapsulation Systems for Delivery of Bioactive Ingredients10.1016/j.foodres.2022.111073 ↗
9. Advances in Micro and Nano Encapsulation of Bioactive Compounds Using Biopolymer and Lipid-Based Transporters10.1016/j.tifs.2018.05.018 ↗
10. Encapsulation of Bioactive Ingredients by Extrusion with Vibrating Technology: Advantages and Challenges10.1007/s11947-019-02326-7 ↗
11. Microencapsulation of Purple Tea Polyphenols Using the Vibrating Nozzle Ionotropic Gelation Technology: Metabolomics, Bioactivity, and Application in Milky Tea10.1016/j.lwt.2024.116099 ↗
12. Production of Stable Food-Grade Microencapsulated Astaxanthin by Vibrating Nozzle Technology10.1016/j.foodchem.2016.10.085 ↗
13. Bosio, A., Beccaria, M., and Lavelli, V. (2026). Ultrasound-Assisted Green Extraction and Hydrogel Encapsulation of Polyphenols from Bean Processing Waste. Foods, 15.10.3390/foods15010030 ↗
14. Optimisation of laminar jet flow microencapsulation variables to enhance the anthocyanin retention and antioxidant function of sohiong10.1039/D6FB00019C ↗
15. Helrich, K. (1990). Animal feed—AOAC official method 930.15—Moisture in Animal Feed. Official Methods of Analysis, AOAC International.
16. Colorimetry of Total Phenolics with Phosphotungstic Acid Reagents10.5344/ajev.1965.16.3.144 ↗
17. Use of a Free Radical Method to Evaluate Antioxidant Activity10.1016/S0023-6438(95)80008-5 ↗
18. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay10.1016/S0891-5849(98)00315-3 ↗
19. Antiplasmodial Activity of Punica granatum L. Fruit Rind10.1016/j.jep.2009.06.025 ↗
20. Characterisation of Pomegranate-Husk Polyphenols and Semi-Preparative Fractionation of Punicalagin10.1002/pca.2691 ↗
21. Doran, P.M. (2013). Chapter 7—Fluid Flow. Bioprocess Engineering Principles, Academic Press. [2nd ed.].10.1016/B978-0-12-220851-5.00007-1 ↗
22. A Standardised Static In Vitro Digestion Method Suitable for Food—An International Consensus10.1039/C3FO60702J ↗
23. Standardization of the Conditions for Extraction of Polyphenols from Pomegranate Peel10.1007/s13197-016-2222-z ↗
24. Microwave-Assisted Extraction of Phenolics from Pomegranate Peels: Optimization, Kinetics, and Comparison with Ultrasounds Extraction10.1016/j.cep.2019.01.006 ↗
25. Magangana, T.P., Makunga, N.P., Fawole, O.A., and Opara, U.L. (2020). Processing Factors Affecting the Phytochemical and Nutritional Properties of Pomegranate (Punica granatum L.) Peel Waste: A Review. Molecules, 25.10.3390/molecules25204690 ↗
26. Phillips, G.O., and Williams, P.A. (2021). Handbook of Hydrocolloids, Woodhead Publishing.
27. Rheology of Food Stabilizers Blends10.1016/j.jfoodeng.2003.09.024 ↗
28. Effects of Sodium Carboxymethyl Cellulose on Rheological Properties and Gelation Behaviors of Sodium Alginate Induced by Calcium Ions10.1016/j.lwt.2018.12.081 ↗
29. Habib, A., Sathish, V., Mallik, S., and Khoda, B. (2018). 3D Printability of Alginate-Carboxymethyl Cellulose Hydrogel. Materials, 11.10.3390/ma11030454 ↗
30. Vibration Technology for Microencapsulation: The Restrictive Role of Viscosity10.4172/2155-9821.1000199 ↗
31. Comparison of Different Technologies for Alginate Beads Production
32. Eskens, O., Villani, G., and Amin, S. (2021). Rheological Investigation of Thermoresponsive Alginate-Methylcellulose Gels for Epidermal Growth Factor Formulation. Cosmetics, 8.10.3390/cosmetics8010003 ↗
33. Microencapsulation using vibrating technology10.3109/02652048.2011.586068 ↗
34. Extraction of Pectin from Watermelon and Pomegranate Peels with Different Methods and its Application in Ice Cream as an Emulsifier10.1111/1750-3841.16752 ↗
35. Díaz-Montes, E. (2023). Wall Materials for Encapsulating Bioactive Compounds via Spray-Drying: A review. Polymers, 15.10.3390/polym15122659 ↗
36. Dundar, A.N., Uzuner, K., Parlak, M.E., Sahin, O.I., Saricaoglu, F.T., and Simsek, S. (2022). Enhanced Functionality and Bio-Accessibility of Composite Pomegranate Peel Extract-Enriched “Boba Balls”. Foods, 11.10.3390/foods11233785 ↗
37. Hashem, N.M., Hosny, N.S., El-Desoky, N., Soltan, Y.A., Elolimy, A.A., Sallam, S.M.A., and Abu-Tor, E.M. (2023). Alginate Nanoencapsulated Synbiotic Composite of Pomegranate Peel Phytogenics and Multi-Probiotic Species as a Potential Feed Additive: Physicochemical, Antioxidant, and Antimicrobial Activities. Animals, 15.10.3390/ani13152432 ↗
38. Encapsulated Pomegranate Peel Extract as a Potential Antimicrobial Ingredient from Food Waste10.1002/jsfa.13698 ↗
39. Effect of Guar Gum-Based Carriers on the Physical and Bioactive Properties of Spray-Dried Delivery Systems of Ground Ivy (Glechoma hederacea L.)10.1016/j.foodhyd.2023.109658 ↗
40. A Process for Turning Pomegranate Peels into a Valuable Food Ingredient using Ultrasound-Assisted Extraction and Encapsulation10.1016/j.ifset.2015.08.006 ↗
41. Effect of Different Extraction Methods on Antioxidant Properties and Encapsulation Efficiency of Anthocyanin of Pomegranate Peel10.1002/fsn3.3362 ↗
42. Sanhueza, L., García, P., Giménez, B., Benito, J.M., Matos, M., and Gutiérrez, G. (2022). Encapsulation of Pomegranate Peel Extract (Punica granatum L.) by Double Emulsions: Effect of the Encapsulation Method and Oil Phase. Foods, 11.10.3390/foods11030310 ↗
43. Encapsulation of Pomegranate Peel Extract in Sucrose Matrix by Co-Crystallization10.1016/j.scp.2022.100949 ↗
44. Nano- and Microstructural Evolution of Alginate Beads in Simulated Gastrointestinal Fluids. Impact of M/G Ratio, Molecular Weight and pH10.1016/j.carbpol.2019.115121 ↗
45. Curcumin Encapsulated Zein/Caseinate-Alginate Nanoparticles: Release and Antioxidant Activity under In Vitro Simulated Gastrointestinal Digestion10.1016/j.crfs.2023.100463 ↗
46. Wong, S.K., Lawrencia, D., Supramaniam, J., Goh, B.H., Manickam, S., Wong, T.W., Pang, C.H., and Tang, S.Y. (2021). In Vitro Digestion and Swelling Kinetics of Thymoquinone-Loaded Pickering Emulsions Incorporated in Alginate-Chitosan Hydrogel Beads. Front. Nutr., 8.10.3389/fnut.2021.752207 ↗
47. Comparison of the use of kinetic model plots and DD solver software to evaluate the drug release from griseofulvin tablets10.22270/jddt.v12i2-S.5402 ↗
48. Kinetic modeling on drug release from controlled drug delivery systems
49. Fickian and non-Fickian diffusion in high polymer systems10.1039/df9704900208 ↗