journal article·2026-05-28Open access

Characterization of Dough Rheological Properties and Bread Quality from Different Triticale Varieties and Fermented Dark Brewers’ Spent Grain

Aliona Ghendov-Mosanu, Iurie Rumeus, Sorina Ropciuc, Olesea Saitan, Viorica Bulgaru, Svetlana Leatamborg, Galina Lupascu, Georgiana Gabriela Codină

Applied Sciences· Volume 16· Issue 11

Abstract

Triticale grains and brewers’ spent grain (BSG) offer promising, sustainable ingredients for bread development, as triticale adapts well to climate change and BSG is a low-cost by-product supporting zero-waste goals. This study evaluated the rheological properties of dough and bread quality obtained from seven triticale cultivars (Ingen 35, Ingen 93, Ingen 40, Ingen 33, Ingen 54, Costel, and Fanica) grown in the Republic of Moldova, with the addition of 5% and 10% fermented dark BSG (BSGF). BSGF incorporation decreased dough stability and protein network strength, as indicated by Mixolab parameters, while the pasting properties varied according to the cultivar. Dynamic rheology showed reductions in storage (G′) and loss (G″) moduli, with tan δ < 1 for all samples. Increasing BSGF levels reduced falling number, Alveograph tenacity, extensibility, baking strength, and Rheofermentometer parameters. In bread, BSGF addition decreased loaf volume and porosity while significantly increasing acidity. Color analysis showed reduced lightness (L*) and increased redness (a*). Texture profile analysis indicated increased hardness and adhesiveness, with stable cohesiveness and reduced resilience. Sensory evaluation revealed improved color and a “hearty” texture at 5% inclusion, whereas 10% resulted in a denser structure and lower acceptability. BSGF significantly influenced the rheological, physicochemical, and sensory properties of triticale bread, highlighting the need for formulation optimization.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Food composition and properties · Polysaccharides Composition and Applications · Wheat and Barley Genetics and Pathology

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Faculty of Food Technology, Technical University of Moldova, 9/9 Studentilor St., MD-2045 Chisinau, Moldova

Bogdan Petriceicu Hașdeu University Center of Cahul, Technical University of Moldova, MD-3909 Chisinau, Moldova

Faculty of Food Engineering, “Stefan cel Mare” University, 720229 Suceava, Romania

Applied Genetics Laboratory, Institute of Genetics, Physiology and Plant Protection, Moldova State University, 20 Padurii St., MD-2002 Chisinau, Moldova

Funding

Ministry of Research, Innovation and Digitization

References (50)

Show source-provided references
1. Andras, B.E., Fițiu, A., Acs, P.B., Horga, V.A., Racz, I., and Duda, M.M. (2024). The Influence of Sowing Rate and Foliar Fertilization on the Yield of Some Triticale Varieties in the Context of Climate Change in Northwest Romania. Agriculture, 14.10.20944/preprints202411.0446.v1 ↗
2. Food and Agriculture Organization of the United Nations (FAO) (2026, February 23). Crops and Livestock Products. Available online: https://www.fao.org/faostat/en/#data/QCL.
3. The influence of conditions on water holding capacity characteristics of triticale varieties planted in the Samarkand region
4. Triticale: Navigating Past Achievements, Current Realities and Future Horizons in Agricultural Innovation
5. Nutritional value in grains of triticale as an alternative in the food industry
6. Triticale, a Grain with Many Uses, Including Medicinal10.15835/hpm.v31i1-2.14741 ↗
7. Effect of bran addition on rheological properties of dough and quality of triticale bread10.1111/jfpp.15093 ↗
8. Pycia, K., Kaszuba, J., Posadzka, Z., and Juszczak, L. (2023). Influence of the Addition of Vital Wheat Gluten on Thermal and Rheological Properties of Triticale Flour. Polymers, 15.10.3390/polym15081870 ↗
9. Tamagno, S., Pittelkow, C.M., Fohner, G., Nelsen, T.S., Hegarty, J.M., Carter, C.E., Vang, T., and Lundy, M.E. (2022). Optimizing water and nitrogen productivity of wheat and triticale across diverse production environments to improve the sustainability of baked products. Front. Plant Sci., 13.10.3389/fpls.2022.952303 ↗
10. Microbial ecology and metabolite dynamics of backslopped triticale sourdough productions and the impact of scale10.1016/j.ijfoodmicro.2023.110445 ↗
11. Current and prospective directions in the use of the grain crops triticale10.52326/jes.utm.2025.32(1).11 ↗
12. Statista Inc (2025). Beer Production Worldwide from 1998 to 2024. Statista, Available online: https://www.statista.com/statistics/270275/worldwide-beer-production/.
13. Potential utilisation methods for brewer’s spent grain: A review10.1093/ijfood/vvae022 ↗
14. Chetrariu, A., and Dabija, A. (2023). Spent Grain: A Functional Ingredient for Food Applications. Foods, 12.10.3390/foods12071533 ↗
15. Farcas, A.C., Chis, M.S., Socaci, S.A., Dulf, F., Podea, P., Salanta, L.C., Paucean, A., and Negrean, O. (2026). Craft Brewers’ Spent Grains as a Secondary Resource: Chemical Profiling of Key Nutritional Components. Agriculture, 16.10.3390/agriculture16070720 ↗
16. In vitro protein digestibility and protein quality assessment of novel brewers’ spent grain protein isolates10.1016/j.ifset.2026.104482 ↗
17. Brewer’s spent grain (BSG) as an ingredient for leavened bread making: Challenges and opportunities10.1016/j.jcs.2025.104223 ↗
18. Quality and Functional Characteristics of Morning Bread Supplemented with Brewer’s Spent Grain Powder10.3746/pnf.2025.259 ↗
19. Valorisation of the brewers’ spent grain through sourdough bread making10.1515/ijfe-2017-0195 ↗
20. Neylon, E., Arendt, E.K., Zannini, E., and Sahin, A.W. (2021). Fermentation as a tool to revitalise brewers’ spent grain and elevate techno-functional properties and nutritional value in high fibre bread. Foods, 10.10.3390/foods10071639 ↗
21. Goh, L.L., and Chi Lik Lee, K. (2021). Biovalorisation of brewer’s spent grain (BSG) and sensory evaluation of BSG bread. ChemRxiv.10.26434/chemrxiv.14491842.v1 ↗
22. Amoriello, T., Mellara, F., Galli, V., Amoriello, M., and Ciccoritti, R. (2020). Technological properties and consumer acceptability of bakery products enriched with brewers’ spent grains. Foods, 9.10.3390/foods9101492 ↗
23. Vriesekoop, F., Haynes, A., van der Heijden, N., Liang, H., Paximada, P., and Zuidberg, A. (2021). Incorporation of fermented brewers spent grain in the production of sourdough bread. Fermentation, 7.10.3390/fermentation7020096 ↗
24. Fibre, protein and mineral fortification of wheat bread through milled and fermented brewer’s spent grain enrichment10.1007/s00217-012-1805-9 ↗
25. Ghendov-Mosanu, A., Ropciuc, S., Dabija, A., Saitan, O., Boestean, O., Paiu, S., Rumeus, I., Leatamborg, S., Lupascu, G., and Codină, G.G. (2025). Effect of Brewers’ Spent Grain Addition to a Fermented Form on Dough Rheological Properties from Different Triticale Flour Cultivars. Foods, 14.10.3390/foods14010041 ↗
26. International Association for Cereal Science and Technology (ICC) (2005). ICC Standard Methods, ICC. 104/1, 136, 105/2, 110/1, 173; ICC121.
27. Codină, G.G., Ursachi, F., Dabija, A., Paiu, S., Rumeus, I., Leatamborg, S., Lupascu, G., Stroe, S.-G., and Ghendov-Mosanu, A. (2025). Physicochemical Properties, Polyphenol and Mineral Composition of Different Triticale Varieties Cultivated in the Republic of Moldova. Molecules, 30.10.3390/molecules30061233 ↗
28. (2007). Wheat Flour. Analysis Methods (Standard No. SR 90:2007).
29. (2007). Bread and Fresh Pastries. Analyses Methods (Standard No. SR 91:2007).
30. (2017). Sensory Analysis—Methodology—General Guidance 2017 (Standard No. ISO 6658:2017).
31. (2007). Sensory Analysis. General Guidance for the Design of Test Rooms (Standard No. ISO 8589:2007).
32. The contribution of fiber components to water absorption of wheat grown in the UK10.1002/cche.10316 ↗
33. Effects of cellulose fiber with different fiber length on rheological properties of wheat dough and quality of baked rolls10.1177/1082013217704122 ↗
34. The effect of different enzymes on the quality of high-fibre enriched brewer’s spent grain breads10.1016/j.foodchem.2008.02.074 ↗
35. Wheat flour non-starch polysaccharides and their effect on dough rheological properties10.1016/j.indcrop.2010.12.003 ↗
36. Influence of cellulosic fibres on pasting properties of starch–fibre blends
37. Zou, X., Wang, X., Li, L., Peng, P., Ma, Q., Hu, X., and Appels, R. (2022). Effects of composition and strength of wheat gluten on starch structure, digestion properties and the underlying mechanism. Foods, 11.10.3390/foods11213432 ↗
38. Ghendov-Mosanu, A., Popa, N., Paiu, S., Boestean, O., Bulgaru, V., Leatamborg, S., Lupascu, G., and Codină, G.G. (2024). Breadmaking Quality Parameters of Different Varieties of Triticale Cultivars. Foods, 13.10.3390/foods13111671 ↗
39. Axentii, M., Stroe, S.-G., and Codină, G.G. (2023). Development and Quality Evaluation of Rigatoni Pasta Enriched with Hemp Seed Meal. Foods, 12.10.3390/foods12091774 ↗
40. Interaction of wheat macromolecules and berry pomace in model dough: Rheology and microstructure10.1016/j.jfoodeng.2017.12.011 ↗
41. Effect of tef [Eragrostis tef (Zucc.) Trotter] grain flour addition on viscoelastic properties and stickiness of wheat dough matrices and bread loaf volume10.1007/s00217-015-2476-0 ↗
42. Verbeke, C., Debonne, E., Versele, S., Van Bockstaele, F., and Eeckhout, M. (2024). Technological evaluation of fiber effects in wheat-based dough and bread. Foods, 13.10.3390/foods13162582 ↗
43. Immobilization of kefir and Lactobacillus casei on brewery spent grains for use in sourdough wheat bread making10.1016/j.foodchem.2007.03.065 ↗
44. Composition and sensory properties of breads supplemented with brewers’ spent grain from three different craft beer styles10.1002/cche.10819 ↗
45. Cheng, J., Zheng, L., Zhao, J., Yu, M., Cao, R., Wang, D., Li, J., and Zhou, L. (2024). Study on the effect of microwaved brewer’s spent grains on the quality and flavor characteristics of bread. Foods, 13.10.3390/foods13030461 ↗
46. Applicability of wheat brewer’s spent grain in steamed bread-making based on physicochemical and visual profiles assessment of doughs and breads
47. Structural recoverability of white breads based on the “cohesiveness” value from texture profile analysis and the “compression force value” from the American Association of Cereal Chemists (74–09)10.1111/jtxs.70028 ↗
48. The effects of different doses of gluten on rheological behaviour of dough and bread quality
49. The eating rate of bread predicted from its sensory texture and physical properties10.1039/D4FO04297B ↗
50. Baiano, A., La Gatta, B., Rutigliano, M., and Fiore, A. (2023). Functional bread produced in a circular economy perspective: The use of brewers’ spent grain. Foods, 12.10.3390/foods12040834 ↗