Optimization of Pulse Repetition Frequency for Laser Inhibition of Microcystis aeruginosa: Insights into Physiological, Biochemical, and Ultrastructural Responses
Source abstract
Abstract
This study elucidates the critical role of pulse repetition frequency (PRF) in optimizing laser inhibition of Microcystis aeruginosa. Using a 355 nm laser (20 ns pulse width, 5 W average power) at 20–65 kHz, 50 kHz is identified as the optimal parameter, achieving 70.6% growth suppression by day 6 (p < 0.001) and reducing cell viability to 28.0 ± 1.6% by day 5 (p < 0.001). Photosynthetic analysis reveals severe PSII dysfunction with Fᵥ/Fₘ of 0.028, representing 91% inhibition (p < 0.001). Biochemical assays demonstrate peak reactive oxygen species generation at 1.59 (p < 0.001) and progressive lipid peroxidation with MDA of 45 nmol/L protein. Transmission electron microscopy and Evans Blue staining corroborate the complete thylakoid disintegration in abundant cells after laser treatment at 50 kHz. These findings establish PRF-dependent photothermal–photomechanical synergy as a deterministic mechanism for efficient, chemical-free algal control.
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Research context
OpenAlex topics: Biocrusts and Microbial Ecology · Algal biology and biofuel production · Light effects on plants
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
State Key Laboratory of Quantum Optics Technologies and Devices, Institute of Laser Spectroscopy, College of Physics and Electronic Engineering, Shanxi University, Taiyuan 030006, China
Funding
Key Research and Development Program of Shanxi Province · Xinzhou Key Research and Development Project
References (53)
Show source-provided references
1. Marine harmful algal blooms (HABs) in the United States: History, current status and future trends10.1016/j.hal.2021.101975 ↗
2. Harmful algal blooms in inland waters10.1038/s43017-024-00578-2 ↗
3. Harmful algal blooms in China: History, recent expansion, current status, and future prospects10.1016/j.hal.2023.102499 ↗
4. Occurrence of antibiotics in waters, removal by microalgae-based systems, and their toxicological effects: A review10.1016/j.scitotenv.2021.151891 ↗
5. Harmful Cyanobacterial Blooms: Biological Traits, Mechanisms, Risks, and Control Strategies10.1146/annurev-environ-112320-081653 ↗
6. A critical review of operational strategies for the management of harmful algal blooms (HABs) in inland reservoirs10.1016/j.jenvman.2022.117141 ↗
7. Huang, H., Wu, G., Sheng, C., Wu, J., Li, D., and Wang, H. (2020). Improved Cyanobacteria Removal from Harmful Algae Blooms by Two-Cycle, Low-Frequency, Low-Density, and Short-Duration Ultrasonic Radiation. Water, 12.10.3390/w12092431 ↗
8. Antibiotics degradation by advanced oxidation process (AOPs): Recent advances in ecotoxicity and antibiotic-resistance genes induction of degradation products10.1016/j.chemosphere.2022.136977 ↗
9. Naturally occurring lasso peptides as algicidal agents against Microcystis aeruginosa10.1016/j.jclepro.2022.135136 ↗
10. A comprehensive review on the photocatalytic inactivation of Microcystis aeruginosa: Performance, development, and mechanisms10.1016/j.chemosphere.2022.137239 ↗
11. Lee, H., Xu, V., Diao, J., Zhao, R., Chen, M., Moon, T.S., Liu, H., Parker, K.M., Jun, Y.-S., and Tang, Y.J. (2024). The use of a benign fast-growing cyanobacterial species to control microcystin synthesis from Microsystis aeruginosa. Front. Microbiol., 15.10.3389/fmicb.2024.1461119 ↗
12. Wu, S., Tong, J., Chen, J., Chen, M., Wang, L., Li, S., Hu, Z., and Chen, H. (2024). Characterization of a novel algicidal bacteria Arenibacter sp. strain 6A1 and its application to eliminate harmful algal blooms. Front. Mar. Sci., 10.10.3389/fmars.2023.1287998 ↗
13. Goodrich, S., Canfield, K.N., and Mulvaney, K. (2024). Expert insights on managing harmful algal blooms. Front. Freshw. Sci., 2.10.3389/ffwsc.2024.1452344 ↗
14. UV-C Irradiation for Harmful Algal Blooms Control: A Literature Review on Effectiveness, Mechanisms, Influencing Factors and Facilities10.1016/j.scitotenv.2020.137986 ↗
15. Microalgae-Mediated Synthesis of Functionalized Gold Nanoparticles with High Photothermal Stability10.1021/acssuschemeng.5c07786 ↗
16. Photodynamic Control of Harmful Algal Blooms by an Ultra-Efficient and Degradable AIEgen-Based Photosensitizer10.1016/j.cej.2020.127890 ↗
17. A Novel Cell Disruption Technique to Enhance Lipid Extraction from Microalgae10.1016/j.biortech.2014.04.056 ↗
18. Inactivation of Microalgae in Ballast Water with Pulse Intense Light Treatment10.1016/j.marpolbul.2014.09.006 ↗
19. Rycroft, T., Fernando, B., and Mayo, M.L. (2025). Using an Ultraviolet-Enabled Boat to Reduce Microcystin and Suppress Cyanobacterial Growth in Harmful Algal Bloom-Impacted Surface Waters. Appl. Sci., 15.10.3390/app15126765 ↗
20. Hao, A., Sun, Z., Shi, X., Xia, D., Liu, X., and Iseri, Y. (2025). Allelopathic Suppression of Cyanobacterial Blooms by the Aquatic Plant Vallisneria Natans Enhanced by Red and Blue LED Light Supplementation. Water, 17.10.3390/w17010131 ↗
21. Z-Scheme Membrane CdZnS/TiO2 Heterojunction Photocatalyst for Efficient Photocatalytic Removal of Microcystis aeruginosa under Simulated Sunlight: Adjustable Suspended Depth and Flexible Assembly10.1016/j.jmst.2024.08.021 ↗
22. Comparative Analysis of Pulsed and Continuous Wave Modes in High-Intensity Laser Light Therapy: Implications for Deep Tissue Treatment10.1002/jbio.202400164 ↗
23. Examination of Axonal Injury and Regeneration in Micropatterned Neuronal Culture Using Pulsed Laser Microbeam Dissection10.1039/b927153h ↗
24. Impact of Shock-Induced Cavitation Bubble Collapse on the Damage of Cell Membranes with Different Lipid Peroxidation Levels10.1021/acs.jpcb.1c02483 ↗
25. Fiegler-Rudol, J., Kępa, M., Skaba, D., and Wiench, R. (2025). Er:YAG Laser Energy Optimization for Reducing Single-Species Microbial Growth on Agar Surfaces In Vitro. Pathogens, 14.10.3390/pathogens14121287 ↗
26. Plasma Mediated Ablation of Biological Tissues with Nanosecond-to-Femtosecond Laser Pulses: Relative Role of Linear and Nonlinear Absorption10.1109/2944.577302 ↗
27. Pulsed Laser Ablation-Induced Green Synthesis of TiO2 Nanoparticles and Application of Novel Small Angle X-Ray Scattering Technique for Nanoparticle Size and Size Distribution Analysis10.1186/s11671-016-1608-1 ↗
28. Effect of Laser and Environmental Parameters on Reducing Microbial Contamination of Stainless Steel Surfaces with Nd:YAG Laser Irradiation10.1111/j.1365-2672.2005.02665.x ↗
29. Effects of High Light and Temperature on Microcystis aeruginosa Cell Growth and β-Cyclocitral Emission10.1016/j.ecoenv.2020.110313 ↗
30. Effect of UV-B Radiation on Amino Acids Profile, Antioxidant Enzymes and Lipid Peroxidation of Some Cyanobacteria and Green Algae10.1080/09553002.2020.1793025 ↗
31. Efficient Inactivation of Microcystis aeruginosa by Carbon Microtube-Supported Magnetic Photocatalysts under Visible Light10.1039/D6RA00677A ↗
32. Light Modulates the Effect of Antibiotic Norfloxacin on Photosynthetic Processes of Microcystis aeruginosa10.1016/j.aquatox.2021.105826 ↗
33. Applicability of the MTT Assay for Measuring Viability of Cyanobacteria and Algae, Specifically for Microcystis aeruginosa (Chroococcales, Cyanobacteria)10.2216/07-11.1 ↗
34. The Freshwater Algae Culture Collection at the Institute of Hydrobiology (FACHB): Algal Resources for Fundamental and Applied Research10.1127/1864-1318/2014/0163 ↗
35. Variations in Growth, Photosynthesis, Oxidative Stress and Microcystin Production in Microcystis aeruginosa Caused by Acute Exposure to Benzalkonium Chloride and Benzalkonium Bromide10.1016/j.psep.2023.12.058 ↗
36. Detection of Reactive Oxygen Species (ROS) in Cyanobacteria Using the Oxidant-Sensing Probe 2’,7’-Dichlorodihydrofluorescein Diacetate (DCFH-DA)10.21769/BioProtoc.2545 ↗
37. Wang, X., Zhu, S., Tao, S., Zhang, S., Wang, R., and Zhang, L. (2025). Isolation of a Novel Streptomyces Sp. TH05 with Potent Cyanocidal Effects on Microcystis aeruginosa. Toxins, 17.10.3390/toxins17070354 ↗
38. Zhang, D., Sun, J.-Z., Fu, M.-H., and Li, C.-J. (2024). Photosynthetic Performance and Antioxidant Activity of Gracilariopsis lemaneiformis Are Sensitive to Phosphorus Deficiency in Elevated Temperatures. Front. Mar. Sci., 11.10.3389/fmars.2024.1432937 ↗
39. Gao, K., Cheng, Y., Geng, R., Xiao, P., Zhang, H., Wu, Z., Cai, F., and Li, R. (2024). Revealing the Diversity of Thin Filamentous Cyanobacteria, with the Discovery of a Novel Species, Pegethrix qiandaoensis Sp. Nov. (Oculatellaceae, Oculatellales), in a Freshwater Lake in China. Diversity, 16.10.3390/d16030161 ↗
40. Chen, X.-D., Liu, Y., Yang, L.-M., Hu, X.-Y., and Jia, A.-Q. (2020). Hydrogen Sulfide Signaling Protects Chlamydomonas reinhardtii Against Allelopathic Damage from Cyanobacterial Toxin Microcystin-LR. Front. Plant Sci., 11.10.3389/fpls.2020.01105 ↗
41. Patterns of Cell Death in Freshwater Colonial Cyanobacteria during the Late Summer Bloom10.2216/06-69.1 ↗
42. Transient Photothermal Inactivation of Escherichia Coli Stained with Visible Dyes by Using a Nanosecond Pulsed Laser10.1038/s41598-020-74714-5 ↗
43. Endale, H.T., Tesfaye, W., and Mengstie, T.A. (2023). ROS induced lipid peroxidation and their role in ferroptosis. Front. Cell Dev. Biol., 11.10.3389/fcell.2023.1226044 ↗
44. Juan, C.A., Perez de la Lastra, J.M., Plou, F.J., and Perez-Lebena, E. (2021). The Chemistry of Reactive Oxygen Species (ROS) Revisited: Outlining Their Role in Biological Macromolecules (DNA, Lipids and Proteins) and Induced Pathologies. Int. J. Mol. Sci., 22.10.3390/ijms22094642 ↗
45. Hu, C., and Rzymski, P. (2019). Programmed Cell Death-Like and Accompanying Release of Microcystin in Freshwater Bloom-Forming Cyanobacterium M.: From Identification to Ecological Relevance. Toxins, 11.10.3390/toxins11120706 ↗
46. UV activated peroxymonosulfate synergistically removes Microcystis aeruginosa and Chlorella vulgaris10.1016/j.jece.2025.115379 ↗
47. Femtosecond laser-induced ultrafast growth of volcanic-shaped graphene micropillars10.1016/j.surfin.2024.104359 ↗
48. Assessment of Skin Lesions Produced by Focused, Tunable, Mid-infrared Chalcogenide Laser Radiation10.1002/lsm.22935 ↗
49. Chavan, P., Yadav, R., Sharma, P., and Jaiswal, A.K. (2023). Laser Light as an Emerging Method for Sustainable Food Processing, Packaging, and Testing. Foods, 12.10.3390/foods12162983 ↗
50. Laser-Based Molecular Delivery and Its Applications in Plant Science10.1186/s13007-022-00908-9 ↗
51. Fan, Q., Hu, W., and Ohta, A. (2017). Localized Single-Cell Lysis and Manipulation Using Optothermally-Induced Bubbles. Micromachines, 8.10.3390/mi8040121 ↗
52. Mechanisms of Nanoparticle-Mediated Photomechanical Cell Damage10.1364/BOE.3.000435 ↗
53. Zhou, R., Li, K., Cao, Y., Shi, W., Yang, Y., Tan, L., Hu, R., and Jin, Y. (2024). Experimental Study of Laser-Induced Cavitation Bubbles near Wall: Plasma Shielding Observation. Water, 16.10.3390/w16101324 ↗