journal article·2026-04-21Open access

Effects of Different Sowing Dates on Nutrient and Microbiological Quality of Maize (Zea mays L.)

Piotr Szulc, Katarzyna Ambroży-Deręgowska, Marek Selwet, Roman Wąsala, Karolina Kolańska, Krzysztof Górecki

Applied Sciences· Volume 16· Issue 8

Abstract

The field experiment was conducted in 2016–2018 at the Department of Agronomy of the Poznań University of Life Sciences on the fields of the Research and Education Centre in Gorzyń, Złotniki branch. It was a single-factor experiment with six sowing dates of an ultra-early maize variety: A1—12 April, A2—26 April, A3—10 May, A4—24 May, A5—7 June, and A6—21 June. Seeds of the maize variety ‘Pyroxenia’ were used in the experiment. This variety is characterized by extremely early maturity (FAO 130), rapid initial development and elongation growth. Delaying the maize sowing date from A1 to A2 resulted in a 16.5% reduction in starch content in the silage dry matter, and a 14.6% increase in the ADF (Acid Detergent Fiber) fiber fraction. The difference in milk production per hectare between maize sown on date A1 and date A6 was 14,189.51 kg/ha, representing 97.1%. Delaying the maize sowing date led to an increase in the abundance of Clostridium spp. in silages, which are responsible for increased losses of dry matter, including starch. No butyric acid was detected in the silages as a final product of butyric fermentation. The low abundance of bacteria from the family Enterobacteriaceae in the silages indicated that they were well prepared. Silages prepared from maize sown at later dates were characterized by a higher abundance of undesirable mold fungi, which are responsible for dry matter losses, including starch. The coefficient of determination showed that 38.54% of the variation in silage starch content was explained by variation in mold abundance in the silage. According to the Flieg–Zimmer scale, all silages received a very good rating, regardless of maize sowing date.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Crop Yield and Soil Fertility · Agriculture, Plant Science, Crop Management · Agricultural economics and policies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Agronomy, Poznań University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland

Department of Mathematical and Statistical Methods, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, Poland

Department of Soil Science and Microbiology, Poznań University of Life Sciences, Szydłowska 50, 60-656 Poznań, Poland

Department of Entomology and Environmental Protection, Poznań University of Life Sciences, Dąbrowskiego 159, 60-594 Poznań, Poland

Lubusz Agricultural Advisory Centre, Kalsk 91, 66-100 Sulechów, Poland

References (60)

Show source-provided references
1. Williams, K., Subramani, M., Lofton, L.W., Penney, M., Todd, A., and Ozbay, G. (2024). Tools and Techniques to Accelerate Crop Breeding. Plants, 13.10.3390/plants13111520 ↗
2. Niedbała, G., Tratwal, A., Piekutowska, M., Wojciechowski, T., and Uglis, J. (2022). A Framework for Financing Post-Registration Variety Testing System: A Case Study from Poland. Agronomy, 12.10.3390/agronomy12020325 ↗
3. Ren, Y., Li, Q., Du, X., Zhang, Y., Wang, H., Shi, G., and Wei, M. (2023). Analysis of Corn Yield Predication Potential at Various Growth Phases Using a Process-Based Model and Deep Learning. Plants, 12.10.3390/plants12030446 ↗
4. Abiotic Stress Responses in Maize: A Review10.1007/s11738-021-03296-0 ↗
5. The Potential Scenarios of the Impacts of Climate Change on Egyptian Resources and Agricultural Plant Production10.4236/ojapps.2016.64027 ↗
6. Szulc, P., Ambroży-Deręgowska, K., Waligóra, H., Mejza, I., Grześ, S., Zielewicz, W., and Wróbel, B. (2021). Dry Matter Yield of Maize (Zea mays L.) as an Indicator of Mineral Fertilizer Efficiency. Plants, 10.10.3390/plants10030535 ↗
7. Szulc, P., Bocianowski, J., Nowosad, K., Zielewicz, W., and Kobus-Cisowska, J. (2021). SPAD Leaf Greenness Index: Green Mass Yield Indicator of Maize (Zea mays L.), Genetic and Agriculture Practice Relationship. Plants, 10.10.3390/plants10050830 ↗
8. Ergosterol and Fusarium Mycotoxins Content in Two Maize Cultivars Under Different Forms of Nitrogen Fertilizers10.1111/jph.12842 ↗
9. The Role of Agrotechnical Factors in Shaping the Health of Maize Plants (Zea mays L.)10.15244/pjoes/122447 ↗
10. Plant Growth and Photosynthetic Characteristics of Soybean Seedlings Under Different LED Lighting Quality Conditions10.1007/s00344-020-10131-2 ↗
11. Mikrobiota Kiszonek
12. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae10.1099/ijsem.0.004107 ↗
13. Fungi and mycotoxins in silage: An overview10.1111/jam.12178 ↗
14. Preparaty bakteryjne modyfikujące procesy fermentacji a jakość kiszonek—Przegląd wyników badań
15. Estimating Silage Energy Value and Milk Yield to Rank Corn Hybrids10.1016/S0377-8401(03)00210-4 ↗
16. The identification of earliness classes in sweet corn cultivars with the sums of effective temperatures
17. Mocek, A., Drzymała, S., and Maszner, P. (1997). Geneza, Analiza i Klasyfikacja Gleb, AR Poznań.
18. Elandt, R. (1964). Statystyka Matematyczna w Zastosowaniu do Doświadczalnictwa Rolniczego, PWN.
19. Review on Crucial Parameters of Silage Quality
20. Muck, R.E., and Kung, L. (2007). Silage Production. Forages, Blackwell. [6th ed.].
21. Silage review: Interpretation of chemical, microbial, and organoleptic components of silage10.3168/jds.2017-13909 ↗
22. Ocena jakości i wartości pokarmowej kiszonek z runi łąkowej wybranych gospodarstw Polski południowowschodniej
23. Jiajie, Z., Xiaochen, D., Junfeng, H., Mingjiu, W., and Gentu, G. (2025). Analysis of the formation mechanism and regulation pathway of oat silage off-flavor based on microbial-metabolite interaction network. BMC Microbiol., 25.10.1186/s12866-025-04166-2 ↗
24. Buxton, D.R., Muck, R.E., and Harrison, J.H. (2003). Typical Populations of Bacterial and Fungal Groups on Plants Prior to Ensiling. Silage Science and Technology, American Society of Agronomy, Crop Science Society of America, Soil Science Society of America.10.2134/agronmonogr42 ↗
25. Buxton, D.R., Muck, R.E., and Harrison, J.H. (2003). Microbiology of Ensiling. Silage Science and Technology, American Society of Agronomy, Crop Science Society of America, Soil Science Society of America.10.2134/agronmonogr42 ↗
26. New Insights into the Structure and Function of Phyllosphere Microbiota through High-Throughput Molecular Approaches10.1111/1574-6968.12225 ↗
27. Wróbel, B., Nowak, J., Fabiszewska, A., Paszkiewicz-Jasińska, A., and Przystupa, W. (2023). Dry Matter Losses in Silages Resulting from Epiphytic Microbiota Activity—A Comprehensive Study. Agronomy, 13.10.3390/agronomy13020450 ↗
28. Acetic Acid Increases Stability of Silage Under Aerobic Conditions10.1128/AEM.69.1.562-567.2003 ↗
29. Effects of Different Lactic Acid Bacteria Groups and Fibrolytic Enzymes as Additives on Silage Quality: A Meta-Analysis10.1016/j.biteb.2021.100654 ↗
30. Biochemistry of Ensiling10.2134/agronmonogr42.c3 ↗
31. Farm Silage Facilities and Their Management for the Prevention of Anaerobic Bacteria Spore Contamination in Raw Milk10.3390/dairy2030040 ↗
32. Identification of the Major Yeasts Isolated from High Moisture Corn and Corn Silages in the United States Using Genetic and Biochemical Methods10.3168/jds.2016-11450 ↗
33. Silage Review: Factors Affecting Dry Matter and Quality Losses in Silages10.3168/jds.2017-13837 ↗
34. Effects of Hybrid and Maturity on the Conservation and Nutritive Value of Snaplage10.1016/j.anifeedsci.2021.114899 ↗
35. Influence of Microbial Additive on Microbial Populations, Ensiling Characteristics, and Spoilage Loss of Delayed Sealing Silage of Napier Grass10.5713/ajas.19.0471 ↗
36. Guo, L., Lu, Y., Li, P., Chen, L., Gou, W., and Zhang, C. (2021). Effects of Delayed Harvest and Additives on Fermentation Quality and Bacterial Community of Corn Stalk Silage. Front. Microbiol., 12.10.3389/fmicb.2021.687481 ↗
37. Yang, F., Cai, Y., Guo, X.S., and Zhong, J. (2022). New Microbial Inoculants for Enhancing Fermentation Quality of Silage, Frontiers Media SA.10.3389/978-2-88974-874-7 ↗
38. Drouin, P., Tremblay, J., and Chaucheyras-Durand, F. (2019). Dynamic Succession of Microbiota During Ensiling of Whole-Plant Corn Following Inoculation with Lactobacillus buchneri and Lactobacillus hilgardii Alone or in Combination. Microorganisms, 7.10.3390/microorganisms7120595 ↗
39. Effect of Dry Matter Content on the Microbial Community and on the Effectiveness of a Microbial Inoculant to Improve the Aerobic Stability of Corn Silage10.3168/jds.2021-21515 ↗
40. Time of Day for Harvest Affects the Fermentation Parameters, Bacterial Community, and Metabolic Characteristics of Sorghum-Sudangrass Hybrid Silage
41. Influence of Inoculation with Lactobacillus on Fermentation, Production of 1,2-Propanediol and 1-Propanol as well as Maize Silage Aerobic Stability10.1515/biol-2020-0038 ↗
42. Effects of Hybrid, Kernel Maturity, and Storage Period on the Bacterial Community in High-Moisture and Rehydrated Corn Grain Silages10.1016/j.syapm.2020.126131 ↗
43. Silage Fermentation
44. McDonald, P., Henderson, A.R., and Heron, S.J.E. (1991). The Biochemistry of Silage, Chalcombe Publications. [2nd ed.].
45. Microbial Community Dynamics During Alfalfa Silage with or without Clostridial Fermentation10.1038/s41598-020-74958-1 ↗
46. Chemical Composition and Microflora of Silage from Maize Ensilage with Bacterial and Chemical Additives
47. Huffman, J., Drouin, P., Duniere, L., and LaPointe, G. (2023). Fermentation and microbial community of maize silage inoculated with Lentilactobacillus buchneri NCIMB 40788 and contaminated with Bacillus and Clostridium spore formers. Fermentation, 9.10.3390/fermentation9090837 ↗
48. Yin, H., Zhao, M., Pan, G., Zhang, H., Yang, R., Sun, J., Yu, Z., Bai, C., and Xue, Y. (2023). Effects of Bacillus subtilis or Lentilactobacillus buchneri on aerobic stability, and the microbial community in aerobic exposure of whole plant corn silage. Front. Microbiol., 14.10.3389/fmicb.2023.1177031 ↗
49. Effects of Lactobacillus plantarum and Fibrolytic Enzyme on the Fermentation Quality and In Vitro Digestibility of Total Mixed Rations Silage Including Rape Straw10.1016/S2095-3119(15)61233-3 ↗
50. Silage Review: Mycotoxins in Silage: Occurrence, Effects, Prevention, and Mitigation10.3168/jds.2017-13788 ↗
51. Hygienic Quality of Silages
52. Recent Advances in Silage Microbiology10.23986/afsci.6718 ↗
53. Engineering Aspects of Ensiling10.1016/S1369-703X(02)00130-4 ↗
54. Fungi Isolated from Contaminated Baled Grass Silage on Farms in the Irish Midlands10.1016/j.femsle.2005.04.037 ↗
55. Wilkinson, J.M. (1999, January 5–7). Silage and Health. Proceedings of the 12th International Silage Production in Relation to Animal Performance, Animal Health, Meat and Milk Quality, Uppsala, Sweden.10.1002/1522-7189(199911/12)7:6<221::AID-NT76>3.0.CO;2-H ↗
56. Silage microbiology and its control through additives10.1590/S1516-35982010001300021 ↗
57. Kiszonka z Kukurydzy—Energia z Całej Rośliny
58. Wojceszak, D., Przybył, J., Czajkowski, Ł., Majka, J., and Pawłowski, A. (2022). Effects of harvest maturity on teh chemical and energetic properties of corn stover biomass combustion. Materials, 15.10.3390/ma15082831 ↗
59. Mycotoxins Occurrence and Fungal Populations in Different Types of Silages for Dairy Cows in Spain10.1016/j.funbio.2019.08.006 ↗
60. Occurrence, Prevention and Remediation of Toxigenic Fungi and Mycotoxins in Silage: A Review10.1002/jsfa.7565 ↗