Antibacterial Activities of Selenium-Modified Zn/Al Layered Double Hydroxide and Its Polycarbazole Hybrid
Source abstract
Abstract
This study investigated the antibacterial activities of Se-Al/Zn layered double hydroxide (LDH) and its polycarbazole (PCz) hybrid against Gram-positive Bacillus subtilis and Gram-negative E. coli cells. Antibacterial performances were evaluated using zone of inhibition assays, viable cell counting, and measurement of metabolic activity based on intracellular ATP levels. The collective results showed that both materials exhibited significant antibacterial activity, with PCz–Se–Al/Zn LDH demonstrating enhanced antibacterial activity compared to Se–Al/Zn LDH. Fluorescent live/dead staining and scanning electron microscopy revealed that treatment with either material resulted in loss of metabolic activity and induction of a non-culturable state in bacterial cells, without observable membrane damage or pronounced morphological changes. Possible antibacterial mechanisms of action associated with LDH and PCz–LDH systems are briefly discussed.
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Research context
OpenAlex topics: Layered Double Hydroxides Synthesis and Applications · Fluoride Effects and Removal · Advanced oxidation water treatment
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Pharmaceutical Sciences, Biomanufacturing Research Institute and Technology Enterprise (BRITE), North Carolina Central University, Durham, NC 27707, USA
Department of Chemistry and Biochemistry, North Carolina Central University, Durham, NC 27707, USA
Funding
NSF
References (42)
Show source-provided references
1. Structural Aspects of Layered Double Hydroxides
2. Chapter 13.1 Layered Double Hydroxides
3. Recent progress in layered double hydroxides as adsorbents for water remediation10.1016/j.clay.2017.04.002 ↗
4. Nanostructured modified layered double hydroxides (LDHs)-based catalysts: A review on synthesis, characterization, and applications in water remediation by advanced oxidation processes10.1016/j.cossms.2021.100965 ↗
5. Recent advances in the synthesis and environmental catalytic applications of layered double hydroxides-based materials for degradation of emerging pollutants through advanced oxidation processes10.1016/j.materresbull.2022.111924 ↗
6. Layered double hydroxide nanostructures and nanocomposites for biomedical applications10.1039/C9TB01312A ↗
7. Layered double hydroxides: Recent progress and promising perspectives toward biomedical applications10.1002/advs.202306035 ↗
8. Study of layered double hydroxides as bactericidal materials against Corynebacterium ammoniagenes, a bacterium responsible for producing bad odors from human urine and skin infections10.1016/j.clay.2019.105194 ↗
9. Da Gama, B.M.V., Selvasembian, R., Giannakoudakis, D.A., Triantafyllidis, K.S., McKay, G., and Meili, L. (2022). Layered Double Hydroxides as Rising-Star Adsorbents for Water Purification: A Brief Discussion. Molecules, 27.10.3390/molecules27154900 ↗
10. Jin, W., Lee, D., Jeon, Y., and Park, D.-H. (2020). Biocompatible Hydrotalcite Nanohybrids for Medical Functions. Minerals, 10.10.3390/min10020172 ↗
11. Layered double hydroxides based composite materials and their applications in food packaging10.1016/j.clay.2023.107216 ↗
12. Preparation, characterization and antimicrobial applications of Zn-Fe LDH against MRSA10.1016/j.msec.2016.05.110 ↗
13. The prospect of layered double hydroxide as bone implants: A study of mechanical properties, cytocompatibility and antibacterial activity10.1016/j.clay.2018.08.020 ↗
14. ZnAl layered double hydroxides impregnated with eucalyptus oil as efficient hybrid materials against multi-resistant bacteria10.1016/j.clay.2017.11.017 ↗
15. Zn2Al-CO3 layered double hydroxide: Adsorption, cytotoxicity and antibacterial performances10.1002/slct.202001264 ↗
16. Direct contact, dissolution and generation of reactive oxygen species: How to optimize the antibacterial effects of layered double hydroxides10.1016/j.colsurfb.2022.112623 ↗
17. Layered double hydroxides: A brief review from fundamentals to application as evolving biomaterials10.1016/j.clay.2017.12.021 ↗
18. Facile synthesis of Cu-LDH with different Cu/Al molar ratios: Application as antibacterial inhibitors10.1007/s11164-020-04268-8 ↗
19. CuFe Layered Double Hydroxide as Self-Cascade Nanoreactor for Efficient Antibacterial Therapy
20. Antimicrobial properties of promising Zn–Fe based layered double hydroxides for the disinfection of real dairy wastewater effluents10.1038/s41598-023-34488-y ↗
21. Chamanmalik, M.I., Antony, A.M., Yelamaggad, C.V., Patil, S.A., and Patil, S.A. (2023). Biogenic Silver Nanoparticles/Mg-Al Layered Double Hydroxides with Peroxidase-like Activity for Mercury Detection and Antibacterial Activity. Molecules, 28.10.3390/molecules28155754 ↗
22. Antifungal and antibacterial investigation of quinary Zr Al Fe Co Ni layered double hydroxide and its Al Fe Co Ni quaternary and Fe Co Ni tertiary roots10.1039/D4RA00508B ↗
23. Divalent metal release and antimicrobial effects of layered double hydroxides10.1016/j.clay.2021.106369 ↗
24. Cardinale, A.M., Alberti, S., Reverberi, A.P., Catauro, M., Ghibaudo, N., and Fortunato, M. (2023). Antibacterial and Photocatalytic Activities of LDH-Based Sorbents of Different Compositions. Microorganisms, 11.10.3390/microorganisms11041045 ↗
25. Effect of molecular dimension on gallery height, release kinetics and antibacterial activity of ZnAl layered double hydroxide (LDH) encapsulated with benzoate and its derivatives10.1016/j.clay.2019.105230 ↗
26. Taylor, M., McGowan, N., Callahan, M.M., Rodriguez, C., Adcock, A.F., Kumar, R., Jenkins, J., Yang, L., and Riaz, U. (Discov. Mater., 2025). Selenium-Polycarbazole Modified LDHs: Dual-Mode Antibacterial Catalysts with Tunable Reactive Oxygen Species Generation, Discov. Mater., in press.10.1007/s43939-025-00526-x ↗
27. Nemček, L., Hagarová, I., and Matúš, P. (2024). Layered Double Hydroxides as Next-Generation Adsorbents for the Removal of Selenium from Water. Appl. Sci., 14.10.3390/app14188513 ↗
28. Sorption-desorption of selenite and selenate on Mg-Al layered double hydroxide in competition with nitrate, sulfate and phosphate10.1016/j.chemosphere.2017.04.071 ↗
29. Cost-effective layered double hydroxides/conductive polymer nanocomposites for electrochemical detection of wastewater pollutants10.1016/j.cjac.2024.100368 ↗
30. Layered double hydroxides (LDHs)-conducting polymers (CPs)-based electroactive materials for supercapacitor application10.1007/s10800-023-01899-8 ↗
31. Interaction mode of polycarbazole–titanium dioxide nanocomposite with DNA: Molecular docking simulation and in-vitro antimicrobial study10.1016/j.jphotobiol.2015.09.001 ↗
32. In situ synthesis and characterization of polycarbazole-copper oxide (PCz-CuO) nanocomposite: Investigating superior antibacterial performance and FabI/FabH docking interactions10.1039/D5RA02065D ↗
33. In-situ polymerization of polycarbazole-zinc oxide nanocomposite: An in silico docking model and in vitro antibacterial biomaterial10.1016/j.eurpolymj.2022.111701 ↗
34. CLSI (2009). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, Approved Standard, Clinical and Laboratory Standards Institute. [8th ed.]. CLSI document M07-A8.
35. Silver(I), Mercury(II), Cadmium(II), and Zinc(II) Target Exposed Enzymic Iron-Sulfur Clusters When They Toxify Escherichia Coli10.1128/AEM.07368-11 ↗
36. Inhibition of the cytochrome bd-terminated NADH oxidase system in Escherichia coli K-12 by divalent metal cations10.1111/j.1574-6968.1995.tb07778.x ↗
37. Zinc oxide nanoparticles: A promising nanomaterial for biomedical applications10.1016/j.drudis.2017.08.006 ↗
38. Dwivedi, S., Wahab, R., Khan, F., Mishra, Y.K., Musarrat, J., and Al-Khedhairy, A.A. (2014). Reactive Oxygen Species Mediated Bacterial Biofilm Inhibition via Zinc Oxide Nanoparticles and Their Statistical Determination. PLoS ONE, 9.10.1371/journal.pone.0111289 ↗
39. Oxidative stress, protein damage and repair in bacteria10.1038/nrmicro.2017.26 ↗
40. Delamination and restacking of hybrid layered double hydroxides assessed by in situ XRD10.1016/j.jcis.2004.01.082 ↗
41. Effect of different forms of anionic nanoclays on cytotoxicity10.1166/jnn.2011.3408 ↗
42. Acid treatment of layered double hydroxides containing carbonate10.1002/ejic.201402372 ↗