journal article·2026-02-03Open access

Exploratory Approach Using Laser-Induced Autofluorescence for Upper Aerodigestive Tract Cancer Diagnosis—Three Case Reports

Ruxandra Ioana Stăncălie-Nedelcu, Șerban Vifor Gabriel Berteșteanu, Gloria Simona Berteșteanu, Ionuț Relu Andrei, Adriana Smarandache, Angela Staicu, Tatiana Tozar, Romeo Costin, Raluca Grigore

Applied Sciences· Volume 16· Issue 3

Abstract

Laser-induced autofluorescence (LIAF) spectroscopy is a label-free optical technique sensitive to biochemical and structural tissue properties. Its application in upper aerodigestive tract malignancies is in its early stages. This study evaluates the feasibility of a matrix scan-based LIAF approach for examining differences between normal and malignant tissues. An exploratory case series involving three patients with oropharyngeal malignancies was conducted. Tissue sections from normal and tumor regions were analyzed using LIAF spectroscopy, including intensity and lifetime measurements, implemented through a matrix scanning protocol with fixed excitation, detection sensitivity, and sample thickness. Complementary Fourier-transform infrared (FTIR) spectroscopy was used to qualitatively assess biochemical variations, and spectroscopic findings were correlated with histopathological evaluation. Within individual cases, consistent differences in autofluorescence spectral and lifetime characteristics were observed between benign and malignant tissue regions. FTIR analysis revealed concurrent biochemical variations that qualitatively supported the autofluorescence observations. This exploratory study demonstrates the potential of combining LIAF matrix scan with FTIR spectroscopy to investigate tissue-specific spectral variations in upper aerodigestive tract lesions. The findings are preliminary and motivate further investigation using larger patient groups and clinically relevant acquisition conditions.
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Research context

OpenAlex topics: Photodynamic Therapy Research Studies · Optical Imaging and Spectroscopy Techniques · Oral Health Pathology and Treatment

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department 12-Otorhynolaryngology, Ophtalmology, Faculty of Medicine, “Carol Davila” University of Medicine and Pharmacy, 050474 Bucharest, Romania

Department of ENT, Head and Neck Surgery, Colţea Clinical Hospital, 030167 Bucharest, Romania

Department of ENT, Head and Neck Surgery, “Carol Davila” Emergency Central Military Hospital, 010825 Bucharest, Romania

Laser Department, National Institute for Laser, Plasma & Radiation Physics, 077125 Magurele, Romania

Extreme Light Infrastructure—Nuclear Physics, “Horia Hulubei” National Institute for R&D in Physics and Nuclear Engineering, 30 Reactorului Street, 077125 Magurele, Romania

Funding

Romanian Ministry of Education and Research · National Research Authority · UEFISCDI · Romanian National Nucleu Program LAPLAS VII · PNCDI IV · IFA ELI-RO Program

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