journal article·2026-02-01Open access

Biotechnological Transformation of Tropical Bee Pollen Through Enzymatic and Bacterial Processes: Impact on Composition and Antioxidant Activity

Karol M. Romero-Villareal, Isabella Lobo-Farfan, María Alcalá-Orozco, Juan José Carrascal, Brayan J. Anaya, Diego F. Tirado, Diana C. Mantilla-Escalante

Applied Sciences· Volume 16· Issue 3

Abstract

This study evaluated the effects of bacterial fermentation (Lactiplantibacillus plantarum, CHOOZIT®, and YO-MIX®) and enzymatic hydrolysis (Protamex® and Neutrase® at 1% w/w and 5% w/w) on the proximate composition and antioxidant activity of bee pollen from the Colombian tropical dry forest. Both treatments significantly modified the nutritional profile, increasing moisture content (48–71% fermented; 50–68% hydrolyzed) while reducing protein and carbohydrate fractions. Fermentation produced strain-dependent antioxidant effects: L. plantarum maximized ABTS scavenging, while YO-MIX® 1:1 achieved the highest DPPH activity. Enzymatic hydrolysis demonstrated superior and more consistent improvements across all assays: Neutrase® 1% achieved 8.5-fold ABTS enhancement, while Protamex® 1% maximized DPPH scavenging (8-fold). All enzymatic treatments increased total phenolic content by 70–84%. Protamex® 1% emerged as the optimal treatment, achieving the highest DPPH activity (2689 µM Trolox equivalents/g pollen), substantial antioxidant enhancement across all assays, and preserved nutritional stability (201 kcal/100 g). These findings support the use of mild enzymatic hydrolysis for valorizing Colombian tropical bee pollen as a functional food ingredient with enhanced bioavailability.
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Research context

OpenAlex topics: Bee Products Chemical Analysis · Insect and Pesticide Research · Healthcare and Venom Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Biological Engineering Program, Dirección Académica, Universidad Nacional de Colombia, Sede de La Paz, La Paz 202017, Colombia

Escuela de Ciencias Básicas, Tecnología e Ingeniería (ECBTI), Universidad Nacional Abierta y a Distancia (UNAD), Cartagena de Indias 130002, Colombia

Secretaría de Educación Distrital Cartagena, Cartagena de Indias 130001, Colombia

Grupo de Investigación GIA, Fundación Universitaria Tecnológico Comfenalco, Cartagena de Indias 130015, Colombia

Department of Pharmaceutics and Food Science, School of Pharmacy, Complutense University of Madrid, 28040 Madrid, Spain

Grupo de Investigación GIND, Universidad del Sinú Elías Bechara Zainúm, Seccional Cartagena, Cartagena de Indias 130001, Colombia

Funding

Ministry of Science, Technology, and Innovation (MinCiencias)-Mission-oriented postdoctoral fellowships · Orchid program · Universidad Nacional de Colombia

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