journal article·2026-01-07Open access

Testing a Farm Animal Model for Experimental Kidney Graft Transplantation: Gut Microbiota, Mycobiome and Metabolic Profiles as Indicators of Model Stability and Suitability

Sona Gancarcikova, Vlasta Demeckova, Stanislav Lauko, Maria Rynikova, Vanda Hajduckova, Pavel Gomulec, David Adandedjan, Eva Petrovova, Rastislav Kalanin, Stefan Hulik, Igor Gala, Jozef Brezina, Jaroslav Novotny, Gabriela Conkova Skybova, Jana Katuchova

Applied Sciences· Volume 16· Issue 2

Abstract

The aim of this pilot study was to comprehensively evaluate the gut microbiota, mycobiome, and metabolomic profile of six 4-month-old crossbred pigs (A–F) originating from the same litter and from a specific breeding facility intended for preclinical transplantation experiments, in order to assess their physiological uniformity and identify potential health-related risks prior to inclusion in a kidney transplantation study. The results demonstrated an overall high degree of microbial and metabolic uniformity among the animals, confirming the stability and suitability of the selected breeding source for experimental purposes. At the same time, several individual differences of potential clinical relevance were observed. Animals A, E, and F exhibited signs of microbial and metabolic imbalance, including reduced diversity, increased oxidative activity, and the presence of potentially pathogenic taxa (Porphyromonadaceae bacterium DJF B175, Aspergillus). In contrast, animals B, C, and D showed a balanced metabolic and microbial profile without pathological deviations. The obtained results highlight the importance of preoperative assessment of the gut bacteriome, mycobiome, and metabolome when selecting animals for transplantation experiments. Such a selective screening approach may contribute to the early identification of physiological deviations, reduction of interindividual variability, and increased reliability and translational potential of preclinical studies.
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Research context

OpenAlex topics: Gut microbiota and health · Clostridium difficile and Clostridium perfringens research · Dialysis and Renal Disease Management

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia

Department of Animal Physiology, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Safarik University, 040 01 Kosice, Slovakia

Clinic of Swine, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia

Department of Surgery, Medical Faculty, Pavol Jozef Safarik University, 040 11 Kosice, Slovakia

L. Pasteur University Hospital, 040 11 Kosice, Slovakia

Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia

Department of Transplantation, L. Pasteur University Hospital, 040 11 Kosice, Slovakia

Funding

Slovak Research and Development Agency · Internal Scientific Grant System (VVGS) of Pavol Jozef Safarik University in Kosice

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