journal article·2026-01-07Open access

Multifaceted Characterization of Olive-Associated Endophytic Fungi with Potential Applications in Growth Promotion and Disease Management

Tasos-Nektarios Spantidos, Dimitra Douka, Panagiotis Katinakis, Anastasia Venieraki

Applied Sciences· Volume 16· Issue 2

Abstract

The olive tree hosts diverse endophytic fungi that may contribute to plant protection and growth. In this study, a preliminary screening of olive-associated fungal endophytes was conducted. A total of 67 fungal endophytes were isolated from the leaves and roots of the Greek cultivars Amfissa and Kalamon and identified using morphological and molecular approaches; 28 representative strains were selected for functional evaluation. Dual culture assays revealed substantial antagonistic activity against major phytopathogens, with growth inhibition ranging from 19.05% to 100%. Notably, strains F.KALl.8 and F.AMFr.15 showed the strongest suppression across pathogens. Interaction phenotyping revealed all major interaction types (A, B, C) and subtype C1/C2, with several strains producing pigmentation zone lines or hyphal ridges at contact sites. The assessment of plant growth-related effects using Arabidopsis thaliana as a model system showed that three strains (F.AMFr.15, F.KALr.4, F.KALr.38A) significantly increased seedling biomass (up to ~16% above the control), whereas nine strains caused severe growth reduction and disease symptoms. Beneficial strains also altered root architecture, inhibiting primary root elongation while inducing extensive lateral root formation. Collectively, these findings highlight the functional diversity of olive-associated fungal endophytes and identify promising candidate strains, particularly F.AMFr.15 (identified as Clonostachys sp.), for further host-specific validation as potential biological control and plant growth-promoting agents.
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Research context

OpenAlex topics: Plant-Microbe Interactions and Immunity · Plant Pathogens and Fungal Diseases · Microbial Natural Products and Biosynthesis

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Laboratory of General and Agricultural Microbiology, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece

Laboratory of Plant Pathology, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece

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