journal article·2025-12-31Open access

Effect of Different Downward Loads and Rotational Speeds on the Removal of Gutta-Percha and Root Canal Sealer Using a Nickel-Titanium Rotary Gutta-Percha Removal System: An Ex Vivo Study

Koki Toyoda, Shunsuke Kimura, Keiichiro Maki, Satoshi Omori, Keiko Hirano, Arata Ebihara, Takashi Okiji

Applied Sciences· Volume 16· Issue 1

Abstract

Effective gutta-percha/sealer removal is essential for predictable nonsurgical endodontic retreatment, but the effects of key operational parameters on nickel–titanium rotary removal instruments remain insufficiently understood. This study aimed to examine how varying downward loads and rotational speeds affect the removal efficiency and torque/force generation of the HyFlex Remover. Sixty transparent straight resin canals filled with gutta-percha and AH Plus sealer were prepared using the HyFlex Remover at two rotational speeds (400 and 800 rpm) and three downward loads (2, 3, and 4 N; n = 10/group). The removal rate, calculated using micro-computed tomography, as well as removal time, maximum force, maximum torque, and cumulative torque were recorded and evaluated using two-way analysis of variance with Bonferroni correction (α = 0.05). The removal rate was consistently higher at 400 rpm than at 800 rpm (p < 0.001), while removal time and cumulative torque were greater at 400 rpm (p < 0.001). Maximum torque differed only between the 800-2 N and 800-4 N groups (p = 0.006). Maximum force increased with higher loads (p < 0.001), and at 3 N and 4 N, it was lower at 400 rpm than at 800 rpm (3 N: p = 0.039, 4 N: p < 0.001). Overall, lower downward loads reduced torque but prolonged working time, whereas higher rotational speeds shortened both working time and torque but decreased the removal rate.
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Research context

OpenAlex topics: Endodontics and Root Canal Treatments · Dental materials and restorations · Dental Erosion and Treatment

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Pulp Biology and Endodontics, Division of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Tokyo 113-8510, Japan

Department of Endodontics, The Nippon Dental University School of Life Dentistry at Tokyo, Tokyo 102-8159, Japan

Funding

Institute of Science Tokyo · Japan Society for the Promotion of Sciences

References (40)

Show source-provided references
1. Non-surgical endodontics: Contemporary biomechanical preparation of the root canal system10.1038/s41415-025-8599-1 ↗
2. Removal of root filling materials10.1111/j.1601-1546.2011.00257.x ↗
3. Critical analysis of research methods and experimental models to study removal of root filling materials10.1111/iej.13650 ↗
4. Assessment of root canal filling removal effectiveness using micro-computed tomography: A systematic review10.1016/j.joen.2016.12.008 ↗
5. Present status and future directions: Canal shaping10.1111/iej.13698 ↗
6. Efficacy of three rotary NiTi instruments in removing gutta-percha from root canals10.1111/j.1365-2591.2007.01335.x ↗
7. Evaluation of apically extruded debris during root canal retreatment with several NiTi systems10.1111/iej.12425 ↗
8. Buckling resistance, torque, and force generation during retreatment with D-RaCe, HyFlex Remover, and Mtwo retreatment files10.5395/rde.2023.48.e10 ↗
9. Comparative evaluation of three different retreatment files for gutta-percha removal under confocal laser scanning microscope: An in vitro study10.22271/oral.2025.v11.i2c.2149 ↗
10. Lee, J., Kim, H.C., Kirkpatrick, T., Jaramillo, D.E., Kwak, S.W., and Jeong, J.W. (2025). Effectiveness of nickel-titanium files for retreatment of molars filled with single-cone hydraulic technique using bioceramic sealers: An in vitro study. Materials, 18.10.20944/preprints202502.0963.v1 ↗
11. Kasuga, Y., Kimura, S., Maki, K., Unno, H., Omori, S., Hirano, K., Ebihara, A., and Okiji, T. (2023). Phase transformation and mechanical properties of heat-treated nickel-titanium rotary endodontic instruments at room and body temperatures. BMC Oral Health, 23.10.1186/s12903-023-03550-6 ↗
12. Abdellatif, D., Iandolo, A., Scorziello, M., Sangiovanni, G., and Pisano, M. (2024). Cyclic fatigue of different Ni-Ti endodontic rotary file alloys: A comprehensive review. Bioengineering, 11.10.3390/bioengineering11050499 ↗
13. Evaluation of apically extruded debris from curved root canal filling removal using 5 nickel-titanium systems10.1016/j.joen.2016.03.012 ↗
14. Microbial causes of endodontic flare-ups10.1046/j.1365-2591.2003.00671.x ↗
15. Alves, F.R., Ribeiro, T.O., Moreno, J.O., and Lopes, H.P. (2014). Comparison of the efficacy of nickel-titanium rotary systems with or without the retreatment instruments in the removal of gutta-percha in the apical third. BMC Oral Health, 14.10.1186/1472-6831-14-102 ↗
16. Maki, K., Ebihara, A., Unno, H., Omori, S., Nakatsukasa, T., Kimura, S., and Okiji, T. (2022). Effect of different downward loads on canal centering ability, vertical force, an torque generation during nickel-titanium rotary instrumentation. Materials, 15.10.3390/ma15082724 ↗
17. Maki, K., Ebihara, A., Luo, Y., Kasuga, Y., Unno, H., Omori, S., Kimura, S., and Okiji, T. (2025). Thermal treatment prevents effects of downward loads on the screw-in force generation and canal-centering ability of nickel-titanium rotary instruments. Materials, 18.10.3390/ma18153610 ↗
18. ‘Screw-in’ tendency of rotary nickel-titanium files due to design geometry10.1111/iej.12363 ↗
19. Liu, M., Xiong, S., Tan, F., and Liu, Y. (2017). Less extrusion debris during the retreatment of curved canals using twisted files with higher rotational speeds: An ex vivo study. BMC Oral Health, 17.10.1186/s12903-017-0340-2 ↗
20. Efficacy of Quantec rotary instruments for gutta-percha removal10.1046/j.1365-2591.2000.00340.x ↗
21. Comparison of removal times of Thermafil plastic obturators using Profiles at different speeds10.1016/j.joen.2006.10.004 ↗
22. Evaluation of the ability of 3 reciprocating instruments to remove obturation material: A micro-computed tomography study10.1016/j.joen.2023.12.004 ↗
23. Dimensional change following setting of root canal sealer materials10.1016/S0109-5641(01)00011-2 ↗
24. Microcomputed tomographic analysis of the efficiency of two retreatment techniques in removing root canal filling materials from mandibular incisors10.1038/s41598-023-29156-0 ↗
25. Jo, H.J., Kwak, S.W., Kim, H.C., Kim, S.K., and Ha, J.H. (2021). Torsional resistance of heat-treated nickel-titanium instruments under different temperature conditions. Materials, 14.10.3390/ma14185295 ↗
26. In-depth metallurgical and microstructural analysis of OneShape and heat treated OneCurve instruments
27. Thu, M., Ebihara, A., Adel, S., and Okiji, T. (2021). Analysis of torque and force induced by rotary nickel-titanium instruments during root canal preparation: A systematic review. Appl. Sci., 11.10.3390/app11073079 ↗
28. Ohri, K., Chen, P.Y.-O., and Peters, O.A. (2024). The biomechanics of nickel titanium instrument fracture in root canal therapy: A narrative review. Materials, 17.10.3390/ma17246147 ↗
29. Stress generation during pecking motion of rotary nickel-titanium instruments with different pecking depth10.1016/j.joen.2017.04.013 ↗
30. Hirano, K., Kimura, S., Maki, K., Omori, S., Ebihara, A., and Okiji, T. (2025). Impact of varying amplitudes of upward and downward motion on the torque/force generation, canal-centering ability, and cyclic fatigue resistance of nickel–titanium reciprocating instrument. Appl. Sci., 15.10.3390/app15010288 ↗
31. Impact of pecking amplitude on cyclic fatigue of new nickel-titanium files10.1002/cre2.811 ↗
32. The role of pecking motion depths in dynamic cyclic fatigue resistance: In vitro study10.1016/j.identj.2024.01.014 ↗
33. The effect of rotational speed and the curvature of root canals on the breakage of rotary endodontic instruments10.1097/00004770-200207000-00014 ↗
34. The effect of three rotational speed settings on torque and apical force with vortex rotary instruments in vitro10.1016/j.joen.2011.01.022 ↗
35. Influence of rotational speed on torque/force generation and shaping ability during root canal instrumentation of extracted teeth with continuous rotation and optimum torque reverse motion10.1111/iej.13485 ↗
36. The effect of rotational speed on force and torque during instrumentation of simulated root canals using ProTaper Next rotary files10.4103/sej.sej_154_23 ↗
37. Heat release, time required, and cleaning ability of MTwo R and ProTaper Universal retreatment systems in the removal of filling material10.1016/j.joen.2010.08.013 ↗
38. A critical appraisal of research methods and experimental models for studies on root canal preparation10.1111/iej.13665 ↗
39. Roentgenographic investigation of frequency and degree of canal curvatures in human permanent teeth10.1097/00004770-200203000-00017 ↗
40. Creation of well-balanced experimental groups for comparative endodontic laboratory studies: A new proposal based on micro-CT and in silico methods10.1111/iej.13288 ↗