Microbiological Purity of Autogenous Dental Augmentative Material After Processing with an Alkaline Ethanol Solution—In Vitro Study
Source abstract
Abstract
Introduction: Teeth intended for use as autogenous augmentative material may carry microbiological contamination, which can compromise the safety of regenerative procedures in the oral cavity. Therefore, effective disinfection protocols are crucial to ensure the microbiological purity of dentin-derived graft materials. Objective: This study aimed to evaluate the effectiveness of a 30% alkaline ethanol solution containing 0.5 M sodium hydroxide in eliminating microorganisms from dentin material intended for autogenous augmentation. Materials and Methods: The study included 44 extracted teeth that were processed using the Smart Dentin Grinder procedure. The presence of microorganisms was analysed using standard microbiological methods before and after treatment with the disinfectant solution. Additionally, the potential association between tooth eruption status and the effectiveness of the disinfection process was evaluated using Fisher’s exact test, with odds ratios calculated using the Haldane–Anscombe correction to address zero cell counts. Results: Processing dentin in a 30% alkaline ethyl alcohol solution containing 0.5 M sodium hydroxide is an effective method for eliminating microorganisms, thereby rendering the material completely microbiologically pure. Conclusions: The dentine processing procedure used appears to ensure the production of autogenous material free from microbiological contamination, indicating its potential safety in clinical applications.
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Research context
OpenAlex topics: Oral microbiology and periodontitis research · Legionella and Acanthamoeba research · Endodontics and Root Canal Treatments
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
SP ZOZ MSWiA w Szczecinie, ul. Jagiellońska 44, 70-382 Szczecin, Poland
Department of Human Nutrition, Faculty of Biotechnology and Food Science, Wrocław University of Environmental and Life Sciences, Józefa Chełmońskiego 37, 51-630 Wrocław, Poland
Department of Microbiology, Faculty of Medicine, Wrocław Medical University, Chałubińskiego 4, 50-367 Wroclaw, Poland
Department of Conservative Dentistry with Endodontics, Wrocław Medical University, Krakowska 26, 50-425 Wrocław, Poland
Faculty of Health Sciences, Angelus Silesius Academy of Applied Sciences in Wałbrzych, Zamkowa 4, 58-300 Wałbrzych, Poland
Institute of Low Temperatures and Structure Research, Polish Academy of Sciences, Okólna 2, 50-422 Wrocław, Poland
Department of Pediatric Dentistry and Preclinical Dentistry, Wrocław Medical University, Krakowska 26, 50-425 Wrocław, Poland
Funding
Wroclaw Medical University
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