journal article·2025-12-15Open access

Medium-Chain Fatty Acid Products Derived from Agriculture and Food Production Sidestreams Decrease Cattle Greenhouse Gas Emissions In Vitro

Natalie Arias, Kalliroi Simeonidis, Alexis H. Rooks, Madison M. Dycus, Hualu Zhou, Luciano Pinotti, Grazia Pastorelli, Joseph G. Usack, Jeferson M. Lourenco

Applied Sciences· Volume 15· Issue 24

Abstract

Impacts of including medium-chain fatty acid (MCFA) products in cattle diets on dry matter digestibility (DMD), volatile fatty acid (VFA), and ruminal gas production were assessed in vitro. Two MCFAs—caproic acid (C6) and caprylic acid (C8)—were produced by a novel bioprocess using agriculture and food waste and microencapsulated with maltodextrin for fast release (FR) and gum arabic for slow release (SR) in addition to C6 and C8 salts. The MCFAs were tested alone and in combination at 1% of dietary dry matter, resulting in eighteen treatments, including a control without MCFA. No treatment reduced DMD%, CH4%, or CH4 yield compared to the control. All treatments except T3 (C8 FR) decreased (p ≤ 0.05) CO2% compared to the control. Certain combinations of MCFA products reduced (p < 0.001) total gas yield and CO2 yield compared to the control, with T17 (C6 FR, C6 SR, C8 FR, C8 SR) having the strongest effect: a total gas yield reduction of 13.9% and a CO2 yield reduction of 29.8%. There was a treatment effect (p ≤ 0.05) on all VFA molar proportions, excluding valerate (p = 0.24). Overall, the MCFA products affected several ruminal fermentation parameters and substantially reduced CO2 production.
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Research context

OpenAlex topics: Ruminant Nutrition and Digestive Physiology · Fatty Acid Research and Health · Anaerobic Digestion and Biogas Production

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Animal and Dairy Science, University of Georgia, 425 River Road, Athens, GA 30602, USA

Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, Italy

Department of Food Science and Technology, University of Georgia, 100 Cedar Street, Athens, GA 30602, USA

Funding

Georgia Agricultural Commodity Commission for Beef

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