journal article·2025-12-11Open access

Blood Lactate Dynamics Reveal Distance-Specific Anaerobic Demands in 400 m Sprint Training

Rafal Omelko, Dariusz Mroczek, Mateusz Jopek, Andrzej Mastalerz, Krzysztof Mackala

Applied Sciences· Volume 15· Issue 24

Abstract

This study aimed to determine how anaerobic energy systems are activated during 350 m and 500 m running tests to identify which distance may more effectively develop special endurance for the 400 m run. Eleven elite male 400 m sprinters (6 in the 350 m group and 5 in the 500 m group) participated in the study. Plasma lactate levels were measured at several time points: before starting, after warm-up, immediately before the test, and 1, 3, 12, 20, and 40 min post-exercise. Blood lactate concentration ([La−]) was determined using the enzymatic Sentinel test (Italy). A difference in blood lactate concentration between groups was observed, reaching statistical significance at the 3 min post-exercise time point, although no significant changes were observed at other time points. The 350 m trial was associated with higher peak lactate levels, suggesting a greater acute anaerobic demand, while the 500 m trial required a longer recovery period for lactate clearance and return to homeostasis.
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Research context

OpenAlex topics: Sports Performance and Training · Cardiovascular and exercise physiology · Muscle metabolism and nutrition

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Track and Field, Wroclaw University of Health and Sport Sciences, al. Ignacego Jana Paderewskiego 35, 51-612 Wrocław, Poland

Department of Biological and Motor Bases of Sports, Wroclaw University of Health and Sport Sciences, al. Ignacego Jana Paderewskiego 35, 51-612 Wrocław, Poland

Department of Individual and Team Physical Activities, Wroclaw University of Health and Sport Sciences, al. Ignacego Jana Paderewskiego 35, 51-612 Wrocław, Poland

Faculty of Physical Education, Jozef Pilsudski University of Physical Education in Warsaw, Marymoncka 34, 00-968 Warsaw, Poland

Funding

University of Health and Sport Sciences in Wroclaw, Poland

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