journal article·2025-11-25Open access

The Bactericidal Effect of Calcium Hydroxide and Triple Antibiotic Paste During Regenerative Endodontic Procedures

Narcisa Mandras, Nicolò Pancini, Janira Roana, Marta Gai, Damiano Pasqualini, Vittorio Fenoglio, Valeria Allizond, Giuliana Banche, Nicola Scotti, Mario Alovisi

Applied Sciences· Volume 15· Issue 23

Abstract

This study investigates the bactericidal efficacy and penetration ability inside dentinal tubules of calcium hydroxide (CH) and a modified tri-antibiotic paste (ciprofloxacin, metronidazole, clarithromycin) (TAP) during regenerative endodontic procedures (REPs). The blood clot serving as a biological scaffold was introduced into the root canal to assess its influence on bacterial regrowth. Forty-four human extracted teeth were infected with E. faecalis and divided in four experimental groups (N = 10) with positive and negative controls (N = 4). Samples were treated with either CH or TAP as intracanal dressing. Bacterial viability and depth of penetration were evaluated using confocal laser scanning microscopy (CLSM) after fluorescent vital staining. The same analysis was performed with or without blood clot exposure and the data were analyzed by one-way ANOVA and a post hoc Bonferroni test (p < 0.05). TAP demonstrated significantly stronger bactericidal activity than CH (p = 0.008). However, its efficacy significantly decreased in the presence of blood clot (p = 0.032). CH showed a moderate antibacterial effect, with its efficacy reduced in the presence of blood. Blood clot exposure consistently reduced the bactericidal efficacy in both groups (p = 0.01) and the dye penetration in CH group (p = 0.041). In conclusion, TAP demonstrated superior antibacterial performance compared to CH and blood clot exposure seemed to decrease antimicrobial efficacy and depth of disinfection during REPs.
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Research context

OpenAlex topics: Endodontics and Root Canal Treatments · Dental Erosion and Treatment · Legionella and Acanthamoeba research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Public Health and Pediatrics, University of Turin, 10126 Turin, Italy

Department of Surgical Sciences, Dental School, Endodontics, University of Turin, 10126 Turin, Italy

Department of Molecular Biotechnology and Health Science, MBC—Molecular Biotechnology Center, University of Turin, 10126 Turin, Italy

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