Silver Nanoparticle-Infused Pullulan Films for the Inhibition of Foodborne Bacteria
Source abstract
Abstract
The aim of this research was to examine the antibacterial activity of commercially available silver nanoparticles against foodborne bacteria and to evaluate the properties of pullulan films incorporating these nanoparticles, including their antibacterial activity and selected physical properties. First, the antibacterial activity of silver nanoparticles against foodborne bacteria was investigated. The following parameters were assessed to evaluate the antibacterial activity of silver nanoparticles: minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), percentage antibacterial activity, bacterial survival based on time–kill curves, leakage of DNA and intracellular proteins using spectrophotometric measurements, and changes in bacterial cell morphology using scanning and transmission electron microscopy (SEM and TEM). Pullulan films with silver nanoparticle content ranging from 2 to 32 µg/cm2 were obtained. The films were evaluated for antibacterial activity and physical properties, including macroscopic and microstructural (SEM) observations, thickness, light barrier, and color. Silver nanoparticles at a concentration of 25 µg/mL achieved 100% inhibition of the test bacteria, with destruction of bacterial cells after 3 or 6 h of incubation, depending on the silver nanoparticle concentration. Incorporation of silver nanoparticles into pullulan films, even in lower amounts, resulted in an antibacterial effect. All films had a compact and uniform microstructure and were shiny and flexible. Analysis of variance showed a significant (p < 0.05) effect of the addition of silver nanoparticles on the thickness, transparency, and color of the films. The obtained pullulan films containing silver nanoparticles were characterized by strong inhibitory activity against foodborne bacteria, had a brown color of varying intensity, a uniform microstructure, a smooth surface, and were barriers to UV radiation and visible light.
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Research context
OpenAlex topics: Polysaccharides Composition and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Food Biotechnology and Microbiology, Institute of Food Sciences, Warsaw University of Life Sciences—SGGW (WULS—SGGW), Nowoursynowska Str., 159c, 02-776 Warsaw, Poland
Funding
Regional Operational Programme of the Mazowieckie Voivodeship · Polish Ministry of Science and Higher Education
References (84)
Show source-provided references
1. Methods of Gold and Silver Nanoparticles Preparation
2. Zhang, X.F., Liu, Z.G., Shen, W., and Gurunathan, S. (2016). Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches. Int. J. Mol. Sci., 17.10.3390/ijms17091534 ↗
3. Kaiser, K.G., Delattre, V., Frost, G.W., Buck, G.W., Phu, J.V., Fernandez, T.G., and Pavel, I.E. (2023). Nanosilver: An old antibacterial agent with great promise in the fight against antibiotic resistance. Antibiotics, 12.10.3390/antibiotics12081264 ↗
4. Bactericidal effect of silver nanoparticles against multidrug-resistant bacteria10.1007/s11274-009-0211-3 ↗
5. Antimicrobial effects of silver nanoparticles10.1016/j.nano.2006.12.001 ↗
6. Multiple parameters for the comprehensive evaluation of the susceptibility of Escherichia coli to the silver ion10.1023/A:1009253223055 ↗
7. Synthesis and Antibacterial Properties of Silver Nanoparticles10.1166/jnn.2005.034 ↗
8. Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli10.1128/AEM.02218-06 ↗
9. Nanosilver: A nanoproduct in medical application10.1016/j.toxlet.2007.10.004 ↗
10. Silver nanoparticles as a safe preservative for use in cosmetics10.1016/j.nano.2009.12.002 ↗
11. Recent advances in antimicrobial treatments of textiles10.1177/0040517507082332 ↗
12. Synthesis and food packaging application of silver nano-particles: A review10.1016/j.hybadv.2024.100230 ↗
13. Kraśniewska, K., Galus, S., and Gniewosz, M. (2020). Biopolymers-Based Materials Containing Silver Nanoparticles as Active Packaging for Food Applications–A Review. Int. J. Mol. Sci., 21.10.3390/ijms21030698 ↗
14. Antibacterial activity of silver bionanocoposites synthesized by chemical reduction rout10.1186/1752-153X-6-101 ↗
15. Gherasim, O., Puiu, R.A., Bîrcă, A.C., Burdușel, A.-C., and Grumezescu, A.M. (2020). An Updated Review on Silver Nanoparticles in Biomedicine. Nanomaterials, 10.10.3390/nano10112318 ↗
16. Trotta, F., Da Silva, S., Massironi, A., Mirpoor, S.F., Lignou, S., Ghawi, S.K., and Charalampopoulos, D. (2023). Silver Bionanocomposites as Active Food Packaging: Recent Advances & Future Trends Tackling the Food Waste Crisis. Polymers, 15.10.3390/polym15214243 ↗
17. Thermo-mechanical, rheological, structural and antimicrobial properties of bionanocomposite films based on fish skin gelatin and silver-copper nanoparticles10.1016/j.foodhyd.2016.08.009 ↗
18. Chitosan nanocomposite films based on Ag-NP and Au-NP biosynthesis by Bacillus subtilis as packaging materials10.1016/j.ijbiomac.2014.05.047 ↗
19. Wang, H., Shi, T., Ma, J., Meng, S., Wei, Z., Sun, Y., Wang, H., and Zhou, M. (2025). Chitosan-based nanocomposite films incorporated with AgNPs/porphyrinic MOFs for killing pathogenic bacteria. Int. J Biol. Macromol., 295.10.1016/j.ijbiomac.2025.139584 ↗
20. Ortega, F., Minnaard, J., Arce, V.B., and García, M.A. (2025). Biodegradable starch-based nanocomposite films with laser-synthesized silver nanoparticles: A materials approach for packaging. Int. J. Biol. Macromol., 318.10.1016/j.ijbiomac.2025.144904 ↗
21. Investigation of water vapour permeation and anti-bacterial properties of nano silver loaded cellulose acetate film
22. Development of cellulose-based bactericidal nanocomposites containing silver nanoparticles and their use as active food packaging10.1016/j.jfoodeng.2011.10.030 ↗
23. Maloufi, M., Djelad, A., Mokhtar, A., Reguig, K., Hasnaoui, M.A., Kebir-Medjhouda, Z.A., Ghamni, M., and Sassi, M. (2025). Fabrication and characterization of cellulose-based packaging film with polyethylene glycol and silver nanoparticles for enhanced antimicrobial efficacy. Int. J Biol. Macromol., 308.10.1016/j.ijbiomac.2025.142381 ↗
24. Comparative studies on pullulan synthesis, melanin synthesis and morphology of white mutant Aureobasidium pullulans B-1 and parent strain A.p.-310.1016/j.carbpol.2007.09.009 ↗
25. European Parliament and the Council (EC) (2012). Regulation (EU) No 231/2012 of 9 March 2012 laying down specifications for food additives listed in Annexes II and III to Regulation (EC) No 1333/2008 of the European Parliament and of the Council Regulation (EU) No 231/2012 of the European Parliament and of the Council. Off. J. Eur. Union, L81, 1–295.
26. European Parliament and the Council (EC) (2008). Regulation (EC) No 1333/2008 of 16 December 2008 on food additives. Off. J. Eur. Union, L354, 16–33.
27. Food and Drug Administration (2025, July 20). GRAS Notice No. GRN 000099, Available online: https://www.hfpappexternal.fda.gov/scripts/fdcc/index.cfm?set=grasnotices&id=99.
28. EFSA Panel on Food Additives and Flavourings (FAF), Castle, L., Andreassen, M., Aquilina, G., Bastos, M.L., Boon, P., and Fallico, B. (2002). Re-evaluation of pullulan (E 1204) as a food additive and new application for its extension of use. EFSA J., 23, e9267.
29. Pullulan—Biopolymer with potential for use as food packaging10.1515/ijfe-2019-0030 ↗
30. Advances in pullulan utilization for sustainable applications in food packaging and preservation: A mini-review10.1016/j.tifs.2022.05.001 ↗
31. An alternative hard capsule prepared with the high molecular weight pullulan and gellan: Processing, characterization, and in vitro drug release10.1016/j.carbpol.2020.116172 ↗
32. Physicochemical properties and applications of pullulan edible films and coatings in fruit preservation10.1002/jsfa.883 ↗
33. Luís, Â., Ramos, A., and Domingues, F. (2020). Pullulan films containing rockrose essential oil for potential food packaging applications. Antibiotics, 9.10.3390/antibiotics9100681 ↗
34. Fabrication, structure, and properties of pullulan-based active films incorporated with ultrasound-assisted cinnamon essential oil nanoemulsions10.1016/j.fpsl.2020.100547 ↗
35. The use of pullulan coating enriched with plant extracts from Satureja hortensis L. to maintain pepper and apple quality and safety10.1016/j.postharvbio.2013.12.010 ↗
36. Antimicrobial and antioxidant properties of pullulan film containing sweet basil extract and an evaluation of coating effectiveness in the prolongation of the shelf life of apples stored in refrigeration conditions10.1016/j.ifset.2014.03.006 ↗
37. The antimicrobial activity of pullulan film incorporated with meadowsweet flower extracts (Filipendula ulmariae flos) on postharvest quality of apples10.1016/j.foodcont.2013.09.049 ↗
38. Sakacin A-containing pullulan film: An active packaging system to control epidemic clones of Listeria monocytogenes in ready-to-eat foods10.1111/j.1745-4565.2010.00213.x ↗
39. Synthesis and characterization of pullulan-mediated silver nanoparticles and its antimicrobial activities10.1016/j.carbpol.2013.04.048 ↗
40. Burduniuc, O., Bostanaru, A.-C., Mares, M., Biliuta, G., and Coseri, S. (2021). Synthesis, Characterization, and Antifungal Activity of Silver Nanoparticles Embedded in Pullulan Matrices. Materials, 14.10.3390/ma14227041 ↗
41. Green and Facile Synthesis of Pullulan-Stabilized Silver and Gold Nanoparticles for the Inhibition of Quorum Sensing10.1021/acsabm.1c00964 ↗
42. Study on physical and mechanical properties of the biopolymer/silver based active nanocomposite films with antimicrobial activity10.1016/j.carbpol.2019.115159 ↗
43. Antifungal activity of transparent nanocomposite thin films of pullulan and silver against Aspergillus niger10.1016/j.colsurfb.2012.09.045 ↗
44. Wypij, M., Rai, M., Zemljič, L.F., Bračič, M., Hribernik, S., and Golińska, P. (2023). Pullulan-based films impregnated with silver nanoparticles from the Fusarium culmorum strains JTW1 for potential applications in food industry and medicine. Front. Bioeng. Biotechnol., 11.10.3389/fbioe.2023.1241739 ↗
45. Stability of antimicrobial activity of pullulan edible films incorporated with nanoparticles and essential oils and their impact on Turkey deli meat quality10.21608/jfds.2013.72104 ↗
46. Incorporation of essential oils and nanoparticles in pullulan films to control foodborne patho-gens on meat and poultry products10.1111/1750-3841.12400 ↗
47. Developing fisetin-AgNPs incorporated in reinforced chitosan/pullulan composite-film and its application of postharvest storage in litchi fruit10.1016/j.foodchem.2022.135122 ↗
48. Recent advances of silver nanoparticle-based polymer nanocomposites for biomedical application10.1039/D4RA08220F ↗
49. Unravelling the effects of silver nanoparticles on textiles: A comprehensive toxicological and quantitative analysis10.1186/s44301-025-00006-4 ↗
50. Advancements in copper and silver sintering as interconnect materials in electronics applications: Review10.1007/s10854-025-15835-3 ↗
51. Silva-Angulo, A.B., Zanini, S.F., Rosenthal, A., Rodrigo, D., Klein, G., and Martinez, A. (2015). Comparative study of the effects of citral on the growth and injury of Listeria innocua and Listeria monocytogenes cells. PLoS ONE, 10.10.1371/journal.pone.0114026 ↗
52. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances10.1038/nprot.2007.521 ↗
53. Pharmacodynamics of antimicrobial drugs10.1016/j.idc.2004.04.012 ↗
54. In vitro antibacterial activities of tigecycline and comparative agents by time-kill kinetic studies in fresh Mueller-Hinton broth10.1016/j.diagmicrobio.2007.05.013 ↗
55. Wei, C., Cui, P., and Liu, X. (2023). Antibacterial activity and mechanism of madecassic acid against Staphylococcus aureus. Molecules, 28.10.3390/molecules28041895 ↗
56. Antimicrobial activity of essential oils on the native microflora of organic Swiss chard10.1016/S0023-6438(03)00088-4 ↗
57. Casting antimicrobial packaging films and measuring their physical properties and antimicrobial activity10.1177/875608799701300405 ↗
58. Singh, P., Pandift, P., Mokkapati, V.R.S.S., Garnæs, J., and Mijakovic, I.A. (2020). Sustainable approach for the green synthesis of silver nanoparticles from Solibacillus isronensis sp. and their application in biofilm inhibition. Molecules, 25.10.3390/molecules25122783 ↗
59. Enhanced therapeutic efficacy of biogenic nanosilver-conjugated thymol: In vitro and in vivo evaluation against emerging multi-drug resistant microbes10.1016/j.jddst.2023.104741 ↗
60. Polyvinyl alcohol/silver nanoparticles (PVA/AgNps) as a model for testing the biological activity of hybrid materials with included silver nanoparticles10.1016/j.msec.2012.05.016 ↗
61. In situ synthesis and long-term stabilization of nanosilver in poly(vinyl acetate-co-butyl acrylate-co-neodecanoate) matrix for antibacterial applications10.1016/j.matchemphys.2020.123476 ↗
62. Multifunctional nanocomposites integrated green synthesized amphiphilic chitosan/thyme extract/nanosilver for antimicrobial and anti-biofilm applications10.1016/j.reactfunctpolym.2023.105791 ↗
63. Controlled synthesis of Ag nanoparticles with different morphologies and their antibacterial properties10.1016/j.msec.2012.09.005 ↗
64. Preparation of nanosilver/polymer composites and evaluation of their antimicrobial and antitumor effect10.1039/D4RA08108K ↗
65. Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria10.1016/j.jcis.2004.02.012 ↗
66. Royal jelly: A novel eco-friendly biotemplate for the sustainable green synthesis of antibacterial silver, iron, copper, and zinc nanoparticles10.1016/j.lwt.2025.117797 ↗
67. Singh, A., Gautam, P.K., Verma, A., Singh, V., Shivapriya, P.M., Shivalkar, S., Sahoo, A.K., and Samanta, S.K. (2020). Green synthesis of metallic nanoparticles as effective alternatives to treat antibiotics resistant bacterial infections: A review. Biotechnol. Rep., 25.10.1016/j.btre.2020.e00427 ↗
68. A green and facile approach to durable antimicrobial coating of cotton with silver nanoparticles, whey protein, and natural tannin10.1016/j.porgcoat.2018.03.007 ↗
69. Antibacterial mechanism of silver nanoparticles in Pseudomonas aeruginosa: Proteomics approach10.1039/C7MT00328E ↗
70. Silver nanoparticles as an antimicrobial agent: A case study on Staphylococcus aureus and Escherichia coli as models for Gram-positive and Gram-positive bacteria10.2323/jgam.2016.07.004 ↗
71. Gevorgyan, S., Schubert, R., Falke, S., Lorenzen, K., Trchounian, K., and Betzel, C. (2022). Structural characterization and antibacterial activity of silver nanoparticles synthesized using a low-molecular-weight Royal Jelly extract. Sci. Rep., 12.10.1038/s41598-022-17929-y ↗
72. Polymer/nanosilver composite coatings for antibacterial applications10.1016/j.colsurfa.2012.12.029 ↗
73. Zhou, B., Yang, Y., Yu, L., Song, G., Ge, J., and Du, R. (2025). Characterization of nanosilver antibacterial bacterial cellulose composite membranes coated with montmorillonite and their potential application in food packaging. Int. J. Biol. Macromol., 289.10.1016/j.ijbiomac.2024.138685 ↗
74. Nanosilver-loaded and polysaccharide-based biomaterial for antibacterial activity—In combination with photobiomodulation for effective wound healing applications10.1016/j.matlet.2024.136293 ↗
75. Electrodeposition of chitosan/gelatin/nanosilver: A new method for constructing biopolymer/nanoparticle composite films with conductivity and antibacterial activity10.1016/j.msec.2015.04.031 ↗
76. Pullulan nanocomposites: Effect of nanoparticles and essential oil reinforcement on its performance and food packaging applications10.1016/j.foohum.2023.08.006 ↗
77. Electrospun pullulan/poly(vinyl alcohol)/silver hybrid nanofibers: Preparation and property characterization for antibacterial activity10.1016/j.colsurfa.2013.07.001 ↗
78. Fabrication of porous chitosan films impregnated with silver nanoparticles: A facile approach for superior antibacterial application10.1016/j.colsurfb.2009.10.044 ↗
79. Synthesis, in vitro characterization and antibacterial efficacy of moxifloxacin-loaded chitosan-pullulan-silver-nanocomposite films10.1016/j.jddst.2019.101366 ↗
80. Transparency of polymeric food packaging materials10.1016/j.foodres.2022.111792 ↗
81. The Role of Packaging and Presentation Format in Consumers’ Preferences for Food: An Application of Projective Techniques10.1111/joss.12162 ↗
82. Implications of Light Energy on Food Quality and Packaging Selection10.1016/B978-0-12-394598-3.00002-2 ↗
83. Recent progress on UV-light barrier food packaging films—A systematic review10.1016/j.ifset.2023.103550 ↗
84. Gore, A.H., and Prajapat, A.L. (2022). Biopolymer Nanocomposites for Sustainable UV Protective Packaging. Front. Mater., 9.10.3389/fmats.2022.855727 ↗